What is the effect of each of the 4 types of inhibitors on the initial rate...
What are the different types of enzyme inhibitors, explain with reaction schemes involved
Inhibitors of enzymes have no effect on kinetic constants O All statements O do not directly interact with enzymes O can decrease rates of enzyme-catalyzed reactions
The concentration of substrate X is low. What happens to the rate of the enzyme- catalyzed reaction if the concentration of X is doubled? 15. What effect does an increase in the enzyme concentration have on the rate of an enzyme-catalyzed reaction? 16.
Match each statement with the condition that it describes. Note that "rate" refers to initial velocity V0 where steady state conditions are assumed. [Etotal] refers to the total enzyme concentration and [Efree] refers to the concentration of free enzyme The Michaelis-Menten equation models the hyperbolic relationship between [S] and the initial reaction rate V for an enzyme-catalyzed, single-substrate reaction ES ES EP. The model can be more readily understood when comparing three conditions: [S] << Km, [S] = Km, and...
For an enzyme catalyzed reaction, the initial rate R. was determined at each initial concentration of substrate [S]. The following data were obtained [S], u M/1 Ro, u M/1 2.5 9.8 20.2 31.7 41.2 50.2 60.1 74.3 (a) Use these data to determine Km and Rmax by means of a Line- weaver-Burke-analysis. (b) Analyze the data using the Eadie-Hofstee procedure to determine Km and Rmax. (c) Analyze the data using the Hanes-Wolff procedure to determine Km and Rmax
Enzyme Kinetics Problem The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are given below: 5 27 23 65 1. Estimate V and K from a Michaelis-Menten graph of V versus [S] 2. Use a Lineweaver-Burk plot to analyze the same data. a. Determine V and Ka from the Lineweaver-Burk BONUS: If the total enzyme concentration was I nmol/L, what is K? Enzyme Kinetics Problem The initial rate for an enzyme-catalyzed reaction...
The initial rate, V, of an enzyme catalyzed reaction varies with substrate concentration as follows: 106 x Initial rate, Ms SJ, M 0.020 0.585 0.004 0.495 0.002 0.392 0.001 0.312 0.250 0.00066 Determine Vmax and Km for this reaction
Chapter 7 1. What is the main source of energy for most living organisms? 2. What are the laws that gover the life and death of cells called? 3. Why is an organism's ability to exchange energy with its environment referred to as an open system? 4. What is entropy? 5. What is the second law of thermodynamics? Does the conversion of energy require the use of energy? 6. Define: a. Catabolism b. Anabolism c. Metabolism d. Exergonic e. Endergonic...
Under what circumstances does an enzyme catalyzed reaction rate resemble a non-enzyme catalyzed reaction? At very low concentrations of substrate (Km is greater than S) the Michaelis-Menton equation can be simplified to? At very high concentrations of substrate, the Michaelis-Menton equation can be simplified to? How do you determine the initial rate of reaction
Which of the following statements are true about competitive enzyme inhibitors? a)They cause irreversible covalent modification of the target enzyme. b)They lead to a decrease in apparent Km. c)All of these d)They have significant affinity for the enzyme-substrate complex. e)Their effect on reaction rate can be overcome at high substrate concentrations. f)They lead to a decrease in apparent Vmax. g)None of these