4. What are three differences in the DnaA found in an E. coli cell right before...
4. What are three differences in the DnaA found in an E. coli cell right before initiation of DNA replication verses the DnaA found in an E. coli daughter cell right after binary fission?
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4. What are three differences in the DnaA found in an E. coli
cell right before...
10. The amount of DNA in one E. coli cell is found to be 5 fg...
10. The amount of DNA in one E. coli cell is found to be 5 fg (femtogram, 10-15 g). Given that the molecular weight of one base pair of DNA is 600 Dalton and 1 Dalton equals 1.66 x 10-24 g, how many base pairs of DNA are there in one E. coli cell? A. 1.8 x 106 B. 2 x 106 C. 5 x 106 D. 7.2 x 106 E. None of the above
Wullugard to GFP and bla, what are the genotypes of E. coli before and after transformation?...
Wullugard to GFP and bla, what are the genotypes of E. coli before and after transformation? 2. What was the purpose of transferring the +DNA and DNA tube from ice to hot water to ice? 3. Why were the tubes incubated at room temperature for 10 minutes before spreading their contents onto the agar plates? Why not just inoculate the plates right after the heat shock?
Fill in the Blank 1. DNA replication in E. coli begins at a 245 base pair...
Fill in the Blank 1. DNA replication in E. coli begins at a 245 base pair sequence called • The initiation of DNA replication at this site begins with the binding of the protein, to multiple 9 base pair repeats regions. The association of these proteins generate superhelical tension which unwinds the 13 bp repeat region called the . The next step of initiation involves the loading of the protein (a helicase) onto the 13 bp repeat region by another...
Question 8 4 pts Which statement best describes the overall function/purpose of interphase? Hint: Cell Cycle...
Question 8 4 pts Which statement best describes the overall function/purpose of interphase? Hint: Cell Cycle and Interphase video • Textbook chapter 10 section 10.2 To divide a parent cell into two identical daughter cells. To double the number of chromosomes. To duplicate all of the cellular contents. To convert a haploid nucleus into a diploid nucleus. To condense and package the DNA into tightly wound chromosomes. Which statement does NOT correctly describe the bacterial chromosome? Hint: • Binary Fission...
The bacterium E. Coli is a Gram-negative rod that is 1mM x 2mM. Each E. Coli cell contains...
The bacterium E. Coli is a Gram-negative rod that is 1mM x 2mM. Each E. Coli cell contains a chromosome of 4 x 106bp of double-stranded DNA. Since 1 kilobase pair (kbp) of double-stranded DNA is 0.340mM in length, what is the length of the E. Coli chromosome? (1 kbp = 1000 base pairs, and 1mM = 10-6meters). A single colony-forming unit (CFU) of E. coli contains 109 cells. If you were to join all the E. coli chromosomes contained in that colony together...
4. You have prepared a batch of competent E coli cells and found its transformation efficiency...
4. You have prepared a batch of competent E coli cells and found its transformation efficiency is 106 per μg plasmid DNA. A professional scientists prepared competent E. coli cells having a transformation efficiency of about 108 cells/ g plasmid DNA Calculate % score of your E. coli transformation efficiency (Τ.Ε.).
For the next group of questions consider a diploid cell from a eukaryotic organism with a...
For the next group of questions consider a diploid cell from a eukaryotic organism with a total of ten chromosomes. After one round of the cell cycle is complete you observe a total of four daughter cells. During this cell division occurred and the resulting daughter cells are. mitosis; haploid with ten total chromosomes each mitosis; diploid with five total chromosomes each meiosis; diploid with ten total chromosomes each meiosis; haploid with five total chromosomes each Before the cell divided,...
Question 2 4 pts Match the phase of the cell cycle with the correct function. Confirming...
Question 2 4 pts Match the phase of the cell cycle with the correct function. Confirming that [Choose DNA replication occured properly DNA replication occurs [Choose [Choose DNA separated into two identical (daughter) cells Initial cell growth occurs Mitosis 10:19 | TE canvas.cwu.edu Question 2 4 pts Match the phase of the cell cycle with the correct function. G1 Confirming that DNA replication occured properly S phase DNA replication occurs Mitosis DNA separated into two identical (daughter) cells Initial cell...
Question 1 1 pts A plant-derived protein known as colchicine can be used to poison cells...
Question 1 1 pts A plant-derived protein known as colchicine can be used to poison cells by blocking the formation of the spindle. Which of the following would result if colchicine is added to a sample of cells in G2? The chromosomes would segregate but in a disorderly pattern. The chromosomes would still line up along across the middle of the cell, but the chromatids would not end up segregating The cells would immediately die. Each resultant daughter cell would...
There are some significant differences in how transcription is regulated in prokaryotes like E. coli versus...
There are some significant differences in how transcription is regulated in prokaryotes like E. coli versus eukaryotes. Which of the following statements concerning gene regulation is TRUE only in eukaryotes? (CHOOSE ALL THAT APPLY AND NO INCORRECT ANSWERS) The promoter of a gene can act in a position and distance-independent manner. The genes involved in one process are usually situated next to each other in the genome and are transcribed in one mRNA. The default state of gene transcription is...
Wullugard to GFP and bla, what are the genotypes of E. coli before and after transformation? 2. What was the purpose of transferring the +DNA and DNA tube from ice to hot water to ice? 3. Why were the tubes incubated at room temperature for 10 minutes before spreading their contents onto the agar plates? Why not just inoculate the plates right after the heat shock?
Fill in the Blank 1. DNA replication in E. coli begins at a 245 base pair sequence called • The initiation of DNA replication at this site begins with the binding of the protein, to multiple 9 base pair repeats regions. The association of these proteins generate superhelical tension which unwinds the 13 bp repeat region called the . The next step of initiation involves the loading of the protein (a helicase) onto the 13 bp repeat region by another...
Question 8 4 pts Which statement best describes the overall function/purpose of interphase? Hint: Cell Cycle and Interphase video • Textbook chapter 10 section 10.2 To divide a parent cell into two identical daughter cells. To double the number of chromosomes. To duplicate all of the cellular contents. To convert a haploid nucleus into a diploid nucleus. To condense and package the DNA into tightly wound chromosomes. Which statement does NOT correctly describe the bacterial chromosome? Hint: • Binary Fission...
4. You have prepared a batch of competent E coli cells and found its transformation efficiency is 106 per μg plasmid DNA. A professional scientists prepared competent E. coli cells having a transformation efficiency of about 108 cells/ g plasmid DNA Calculate % score of your E. coli transformation efficiency (Τ.Ε.).
For the next group of questions consider a diploid cell from a eukaryotic organism with a total of ten chromosomes. After one round of the cell cycle is complete you observe a total of four daughter cells. During this cell division occurred and the resulting daughter cells are. mitosis; haploid with ten total chromosomes each mitosis; diploid with five total chromosomes each meiosis; diploid with ten total chromosomes each meiosis; haploid with five total chromosomes each Before the cell divided,...
Question 2 4 pts Match the phase of the cell cycle with the correct function. Confirming that [Choose DNA replication occured properly DNA replication occurs [Choose [Choose DNA separated into two identical (daughter) cells Initial cell growth occurs Mitosis 10:19 | TE canvas.cwu.edu Question 2 4 pts Match the phase of the cell cycle with the correct function. G1 Confirming that DNA replication occured properly S phase DNA replication occurs Mitosis DNA separated into two identical (daughter) cells Initial cell...
Question 1 1 pts A plant-derived protein known as colchicine can be used to poison cells by blocking the formation of the spindle. Which of the following would result if colchicine is added to a sample of cells in G2? The chromosomes would segregate but in a disorderly pattern. The chromosomes would still line up along across the middle of the cell, but the chromatids would not end up segregating The cells would immediately die. Each resultant daughter cell would...
There are some significant differences in how transcription is regulated in prokaryotes like E. coli versus eukaryotes. Which of the following statements concerning gene regulation is TRUE only in eukaryotes? (CHOOSE ALL THAT APPLY AND NO INCORRECT ANSWERS) The promoter of a gene can act in a position and distance-independent manner. The genes involved in one process are usually situated next to each other in the genome and are transcribed in one mRNA. The default state of gene transcription is...
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