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(4 pts) You are designing new PCR primers for MLST and need to identify a gene to U r these primers. Describe two parameters of the gene that are important to conside your design
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Answer #1

First parameter is the GC content of the strand. The more the GC content the more is the the temperature required for denaturation of the two strands.

Second we must make sure that there are not secondary structures in the the gene or else faulty or no amplification will occur

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