what is the co factor of restriction endonulease EcoRV that activiates water for nuceophilic attack?
what is the co factor of restriction endonulease EcoRV that activiates water for nuceophilic attack?
Homework Answers
Magnesium ion (Mg2+).
The magnesium ion holds a water molecule in a position from which the water molecule can attack the phosphoryl group and, in conjunction with the aspartate residues, helps polarize the water molecule toward deprotonation.Additional studies have revealed that a second magnesium ion must be present in an adjacent site for EcoRV endonuclease to cleave its substrate.
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what is the co factor of restriction endonulease EcoRV that
activiates water for nuceophilic attack?
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the...
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3' 3' CCGG 5 5' AAGCTT 3 3' TTCGAA 5 5' RGGWCCY 3 3' YCCWGGR 5 Cleavage 5G AATTC 3' 3' CTTAA G 5'...
Need Part C answered only. The table shows where different restriction endonucleases (restriction enzymes) cleave DNA....
Need Part C answered only.
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3' 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3 3' CCGG 5 5' AAGCTT 3' 3' TTCGAA 5 5'RGGWCCY 3 3' YCCWGGR 5 Cleavage 5'G AATTC 3'...
4 - The restriction enzyme EcoRV is not active if it has not bound to its...
4 - The restriction enzyme EcoRV is not active if it has not bound to its target site. Describe how the enzyme is activated upon binding to its correct target site, and describe the biological importance of this activation mechanism.
ng Learning The following table shows where different restriction endonu abbreviation R represents the purines (adenine...
ng Learning The following table shows where different restriction endonu abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine cleases (restriction enzymes) cleave DNA. The Cleavage 5' G EcoRI 5 GAATTC 3 AATTC 3 5' 3 CTTAAG 5' EcoRV 5 GATATC 3 3' CTATAG 5 3' CTTAA 5 GAT ATC 3 Hae? Hindlll PpuMI5' RGGWCCY 3 3' CTA TAG 5 ?'CC GG5. 5 A...
Question 2: EcoRI, EcoRII, and EcoRV are all restriction enzymes found in E.coli. How does E.coli...
Question 2: EcoRI, EcoRII, and EcoRV are all restriction enzymes found in E.coli. How does E.coli prevent these enzymes from digesting its own DNA? Question 3: When performing a restriction digest it is necessary to incubate the enzymes and the DNA together to give the enzymes time to work. Given that we're using enzymes isolated from E.coli, a human symbiont, what temperature would you incubate the enzymes at, and why? Question 4: Why do DNA fragments migrate across the gel...
Which of the following restriction endonucleases produces 3' overhangs? (position where the enzyme cuts is indicated...
Which of the following restriction endonucleases produces 3' overhangs? (position where the enzyme cuts is indicated by an arrow) A. EcoRI (5'-G↓AATTC-3') B. HindIII (5'-A↓AGCTT-3') C. EcoRV (5'-GAT↓ATC-3') D. PstI (5'-CTGCA↓G-3')
(CO E) A zombie computer is one that can result in a denial of service attack...
(CO E) A zombie computer is one that can result in a denial of service attack and a botnet attack. a botnet attack. no attack because there is no such thing as a zombie computer. an acceptance of all services.
What is calorie restriction? How is calorie restriction important? What can calorie restriction prevent? How can...
What is calorie restriction? How is calorie restriction important? What can calorie restriction prevent? How can calorie restriction affect overall health?
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this...
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this plasmid into two fragments. A. True B. False 2.In general, restriction enzymes that recognize four nucleotides have higher probability to produce more DNA fragments than those enzymes that recognize six nucleotides. A. true B. false 3. Which of the following sequences are palindromes? A. 5' TGGCCA 3' B. 5' GAAAAG 3' C. 5' CGATGG 3' D. 5' GACGAC 3' 4. Below are the possible...
10) I was asked to perform double digestion of a given plasmid (map below) with restriction...
10) I was asked to perform double digestion of a given plasmid (map below) with restriction enzymes EcoRI and HindITI so that I can perform ligation of gene of interest into another vector cut by same enzymes. While setting up digestion reaction I was talking to my friend and mistakenly added EcoRV instead of EcoRI. Genere Please draw bands on gel (hypothetical sizes) L UC C LEDNA Ladder UC = Uncut Plasmid C = Cut Plasmid TIITTIIIII 1000
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3' 3' CCGG 5 5' AAGCTT 3 3' TTCGAA 5 5' RGGWCCY 3 3' YCCWGGR 5 Cleavage 5G AATTC 3' 3' CTTAA G 5'...
Need Part C answered only.
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3' 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3 3' CCGG 5 5' AAGCTT 3' 3' TTCGAA 5 5'RGGWCCY 3 3' YCCWGGR 5 Cleavage 5'G AATTC 3'...
ng Learning The following table shows where different restriction endonu abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine cleases (restriction enzymes) cleave DNA. The Cleavage 5' G EcoRI 5 GAATTC 3 AATTC 3 5' 3 CTTAAG 5' EcoRV 5 GATATC 3 3' CTATAG 5 3' CTTAA 5 GAT ATC 3 Hae? Hindlll PpuMI5' RGGWCCY 3 3' CTA TAG 5 ?'CC GG5. 5 A...
Question 2: EcoRI, EcoRII, and EcoRV are all restriction enzymes found in E.coli. How does E.coli prevent these enzymes from digesting its own DNA? Question 3: When performing a restriction digest it is necessary to incubate the enzymes and the DNA together to give the enzymes time to work. Given that we're using enzymes isolated from E.coli, a human symbiont, what temperature would you incubate the enzymes at, and why? Question 4: Why do DNA fragments migrate across the gel...
10) I was asked to perform double digestion of a given plasmid (map below) with restriction enzymes EcoRI and HindITI so that I can perform ligation of gene of interest into another vector cut by same enzymes. While setting up digestion reaction I was talking to my friend and mistakenly added EcoRV instead of EcoRI. Genere Please draw bands on gel (hypothetical sizes) L UC C LEDNA Ladder UC = Uncut Plasmid C = Cut Plasmid TIITTIIIII 1000
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