What does it mean when two bands appear the same in two restriction cut DNAs?
Homework Answers
In gel-electrophoresis, the fragments of DNA or RNA migrate under the influence of electric field. The separation of these fragments is accomplished by exploiting the mobilities with which different sized molecules are able to pass through the gel. Larger molecules (higher mol wt.) move slower than the smaller molecules (lower mol wt.). Therefore, if the mol wt of the fragments of two restriction cut DNAs are the same then their bands will appear the same on the gel. If these fragments are run on the same gel then you will only see one band corresponding to the mol wt of the fragments of the DNAs i.e. two distinct bands for two different DNA fragments would not be visible.
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What does it mean when two bands appear the same in two restriction
cut DNAs?
A restriction map lists the locations of DNA sequences that are cut by a particular restriction...
A restriction map lists the
locations of DNA sequences that are cut by a particular restriction
enzyme for a piece of DNA, such as a chromosome or a plasmid.
Restriction maps are important when generating a construct for
experimental use. Digesting the DNA sequence with the restriction
enzymes will result in fragmented DNA of predictable sizes, based
on the restriction map, that allow a researcher to analyze if his
or her construct was generated correctly when visualized using gel
electrophoresis....
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. Isoschizomers are restriction enzymes that recognize the same sequence, but may not cut in the same position. You want to cut at the site G/GCGCC, but do not have SspDI. What isoschizomer could you use to cut in the same position? (Hint: Use the Enzyme Finder tool at New England Biolabs website) (1 point)
You cut the pBad-mTagBFP2 plasmid with the BamHI restriction enzyme only. There is only one BamHI...
You cut the pBad-mTagBFP2 plasmid with the BamHI restriction enzyme only. There is only one BamHI RE site. How many bands do you expect to see after you run the product on a gel? Would you expect the same result if you cut a linear PCR product that also only has one cut site? Explain your reasoning
1.) If we mix two restriction fragments cut with the same restriction enzyme in the presence of DNA ligase, ATP and appropriate buffer, we will obtain... A) protein B) carbohydrate C) recombinant DNA...
1.) If we mix two restriction fragments cut with the same restriction enzyme in the presence of DNA ligase, ATP and appropriate buffer, we will obtain... A) protein B) carbohydrate C) recombinant DNA or plasmid D) pAMP
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this...
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this plasmid into two fragments. A. True B. False 2.In general, restriction enzymes that recognize four nucleotides have higher probability to produce more DNA fragments than those enzymes that recognize six nucleotides. A. true B. false 3. Which of the following sequences are palindromes? A. 5' TGGCCA 3' B. 5' GAAAAG 3' C. 5' CGATGG 3' D. 5' GACGAC 3' 4. Below are the possible...
Use the diagram below for questions 9-10. The black line indicates a locus, arrows indicate restriction sites and the red line indicates a probe to the region indicated. What does the arrow with the...
Use the diagram below for questions 9-10. The black line indicates a locus, arrows indicate restriction sites and the red line indicates a probe to the region indicated. What does the arrow with the star/asterisk above it mean? Choose all possible answers. All of these answers are correct. None of these answers are correct. That restriction site is a SNP. That restriction site is a RFLP. That site will not vary between homologs within an individual. That site will not...
24. What do restriction enzymes do? A Randomly cut DNA B. Produce protein C. Cut DNA...
24. What do restriction enzymes do? A Randomly cut DNA B. Produce protein C. Cut DNA at a specific nucleotide sequence D. Copy DNA 25. Which of the following is an example of a differentiated cell? A. Embryonic Stem Cell B. Hematopoeitic Stem Cell C. Adult Stem Cell D. Liver Cell 26. An example of a small, circular DNA molecule used as a vector to transfer foreign DNA to a host cell is a A. plasmid B prion C. liposome...
What does Foot mean when she claims that the word good means the same thing when...
What does Foot mean when she claims that the word good means the same thing when applied to features of humans as when applied to features of plants? Do you think she is right?
Two experiments were performed in order to confirm the Beta-glucuronidase gene from E. coli was present...
Two experiments were performed in order to confirm the
Beta-glucuronidase gene from E. coli was present in the pET28a
plasmid. (Lane1) PCR was performed to amplify the
Beta-glucuronidase gene and if the gene was present then a single
band would appear at ~7,300 bp. However, the band appeared to drag
down the gel but stopped ~7,300 bp. What would cause the PCR
product to smear down the gel and does this mean that the
amplification of the PCR product was...
If your DNA was not cut to completion during the restriction analysis of your transformants, what...
If your DNA was not cut to completion during the restriction analysis of your transformants, what would you expect your gel to look like? Would you still be able to distinguish positive from negative clones?
A restriction map lists the
locations of DNA sequences that are cut by a particular restriction
enzyme for a piece of DNA, such as a chromosome or a plasmid.
Restriction maps are important when generating a construct for
experimental use. Digesting the DNA sequence with the restriction
enzymes will result in fragmented DNA of predictable sizes, based
on the restriction map, that allow a researcher to analyze if his
or her construct was generated correctly when visualized using gel
electrophoresis....
Use the diagram below for questions 9-10. The black line indicates a locus, arrows indicate restriction sites and the red line indicates a probe to the region indicated. What does the arrow with the star/asterisk above it mean? Choose all possible answers. All of these answers are correct. None of these answers are correct. That restriction site is a SNP. That restriction site is a RFLP. That site will not vary between homologs within an individual. That site will not...
24. What do restriction enzymes do? A Randomly cut DNA B. Produce protein C. Cut DNA at a specific nucleotide sequence D. Copy DNA 25. Which of the following is an example of a differentiated cell? A. Embryonic Stem Cell B. Hematopoeitic Stem Cell C. Adult Stem Cell D. Liver Cell 26. An example of a small, circular DNA molecule used as a vector to transfer foreign DNA to a host cell is a A. plasmid B prion C. liposome...
Two experiments were performed in order to confirm the
Beta-glucuronidase gene from E. coli was present in the pET28a
plasmid. (Lane1) PCR was performed to amplify the
Beta-glucuronidase gene and if the gene was present then a single
band would appear at ~7,300 bp. However, the band appeared to drag
down the gel but stopped ~7,300 bp. What would cause the PCR
product to smear down the gel and does this mean that the
amplification of the PCR product was...
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