Nicotiana tabacum cells are cultured to high density for production of polysaccharide gum. The reactor used...
Nicotiana tabacum cells are cultured to high density
for production of polysaccharide gum. The reactor used is a stirred
tank that initially contains 100 litres of medium. The maximum
specific growth rate of the culture is 0.18 day-1 and the yield of
biomass from substrate is 0.5 g g-1. The concentration of
growth-limiting substrate in the medium is 3% (w/v). The reactor is
inoculated with 1.5 g L-1 of cells and operated in batch until
the
substrate is virtually exhausted; medium flow is then started at a
rate of 4 L day-1. The biomass concentration is 16.4 g
L−1 after 13.3 days of batch operation. Fed-batch
operation is carried out for 40 days under quasi-steady-state
conditions after batch operation is done. Determine the final mass
of cells after 40 days of fed-batch culture.
from my understanding the answer is 4.4kg, no idea how to formulate the equation to get to that answer
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Nicotiana tabacum cells are cultured to high density
for production of polysaccharide gum. The reactor used...
help me answer quetion 1 please 11 42% & 17:19 4/ Homework 2- ECH160,pdf Quarter 2,...
help me answer quetion 1 please
11 42% & 17:19 4/ Homework 2- ECH160,pdf Quarter 2, 2018-2019 ECH 160Homework 2 Instructor: N.T Linh, Dept. OR&P, HUMG Assigned on 24 May, 2019 Due on 27 May, 2019 Problem 1 A mouse-mouse hybridoma cell line is used to produce monoclonal antibody. Growth in batch culture is monitored with the following results. Time (day) Cell concentration (cells/ml) x 10 0.0 0.45 0.2 0.52 0.65 0.5 0.81 1.5 1.22 1.77 2.13 3.0 3.55 4.02...
3. The bacterium Corynebacterium glutamicum has been aerobically cultured in a batch fermenter vessel on glucose...
3. The bacterium Corynebacterium glutamicum has been aerobically cultured in a batch fermenter vessel on glucose at pH 7.0 and 20 °C to produce Glutamic acid for the manufacture of Mono Sodium Glutamate, a flavour compound for the food industry. The time course data for a typical batch culture of this bacterium in a fermenter vessel are given below: t (h) Cell dry weight (g l-1) Glucose concentration (g l-1) Glutamic acid concentration (g l-1) 0 1.22 100 0 8 ...
A CHO mammalian cell culture is set up by a chemostat stirred tank bioreactor to produce...
A CHO mammalian cell culture is set up by a chemostat stirred tank bioreactor to produce HBsAg. Glucose is the growth rate limited substrate and cell growth follows the Monod Model. The feeding flow rate is 20L/hr with the glucose concentration 0.25M, and the glucose concentration in the outlet is 5g/L. It has been found out the maximal cell growth rate is 0.2 hr-1 and the saturation constant, or half-velocity constant of substrate is 2.0 g/L. The cell yield coefficient...
o) A two stage chemostate system is used for production of se 3 ry metabolite. The volume of each reactor is 2 m'...
o) A two stage chemostate system is used for production of se 3 ry metabolite. The volume of each reactor is 2 m'; the flow rate of feed is 200 ocurs in the both ractors but the scecond reactor is used for L/h. Mycelial growth for product synthesis. The concentration of substrate in the feed is 20 g/1. Kinetic and yield parameters for the organisms are: Yxs = 0.5 kg/kg Ks 1.0 kg/m Hm 0.12/h ms = 0.025 kg/kg-h p...
You are looking to design the first couple of units in a fermentation suite. The cells and produc...
You are looking to design the first couple of units in a fermentation suite. The cells and product are particularly delicate. The cells need a substrate, S, that is extremely expensive. However we can make substrate S from a cheap reactant, A, and a slightly expensive reactant, B. At the end of the exam is a diagram of the whole system. Species A is continously fed into a reactor (Caf 1 mol/ vo 2 L/ s) initilly charged with species...
Please summarise the following content:(no word limit) EXPERIMENTAL PROCEDURES Animals All animals including pregnant ICR mice,...
Please summarise the following content:(no word
limit)
EXPERIMENTAL PROCEDURES Animals All animals including pregnant ICR mice, neonatal Wistar rats, and NOD-SCID mice (8 weeks old, male) were purchased from CLEA Japan (Tokyo). All exper- imental procedures and protocols were approved by the Animal Care and Use Committee of Keio University and conformed to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Cells Mouse ESCs were obtained from the Laboratory of Pluripotent Cell Studies, RIKEN...
reply ma as soon as possible 120 points total Due: October 23, 2019 Budget your time...
reply ma as soon as possible
120 points total Due: October 23, 2019 Budget your time carefully TRUE OR FALSE (45 points - 3 points cach): Mark whether the statements as written are "true" or "false". If the statement is false, make minor changes to the statement so that the statement is true. The primary adverse health effect resulting from the disposal of mercury wastes into Minamata Bay was dysentery The flow regime in horizontal velocity grit chambers approaches continuous...
The Jounal of Immunol nes of the National Academy of Sciences USA 89. 6550-6554. 01992, by permis...
explain figure 2 please on the third page
The Jounal of Immunol nes of the National Academy of Sciences USA 89. 6550-6554. 01992, by permision of Proc. Natl. Acad. Sci. USA Vol. 89, pp. 6550-63554, July 1992 Immunolog A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells RANDOLPH J. NOELLE时, MEENAKSHI ROY.. DAVID M. SHEPHERD., IVAN STAMENKOVICt JEFFREY A. LEDBETTER, AND ALEJANDRO ARUFFo Departmend Microbiology. Dartmouth Medical School One...
please help me. answer only first page. thank you! p. If you accidentally forgot to inoculate...
please help me. answer only first page. thank you!
p. If you accidentally forgot to inoculate your organism in an OF-glucose tube with oil, and only had one yellow “no oil” tube, what type of carbohydrate catabolism would describe that organism (fermenter, oxidative, neither)? Explain your answer. q. What are two other results from the OF-glucose tubes other than their type of carbohydrate catabolism? r. How can organisms that don't use starch grow on a starch agar plate? Carbohydrate Catabolism...
LAR STUDY GUM Exercises 76, 77, 78, 79,719, 7.11.8.19.2.0.7.0 1. Define niche A ecological envi B...
LAR STUDY GUM Exercises 76, 77, 78, 79,719, 7.11.8.19.2.0.7.0 1. Define niche A ecological envi B by-product of microbial metabolic activities C icorial growth D host-preste relationship E None of the above 2.Microbes muy compete ayust the environment tempera requirements in order to compete for nutrients A True B. False , c.) to the prima A B The pathogenesis of disease can move son of issues by extracellule enzymes or physical blocking of capillaries due to unlimited microbial greneth True...
help me answer quetion 1 please
11 42% & 17:19 4/ Homework 2- ECH160,pdf Quarter 2, 2018-2019 ECH 160Homework 2 Instructor: N.T Linh, Dept. OR&P, HUMG Assigned on 24 May, 2019 Due on 27 May, 2019 Problem 1 A mouse-mouse hybridoma cell line is used to produce monoclonal antibody. Growth in batch culture is monitored with the following results. Time (day) Cell concentration (cells/ml) x 10 0.0 0.45 0.2 0.52 0.65 0.5 0.81 1.5 1.22 1.77 2.13 3.0 3.55 4.02...
o) A two stage chemostate system is used for production of se 3 ry metabolite. The volume of each reactor is 2 m'; the flow rate of feed is 200 ocurs in the both ractors but the scecond reactor is used for L/h. Mycelial growth for product synthesis. The concentration of substrate in the feed is 20 g/1. Kinetic and yield parameters for the organisms are: Yxs = 0.5 kg/kg Ks 1.0 kg/m Hm 0.12/h ms = 0.025 kg/kg-h p...
You are looking to design the first couple of units in a fermentation suite. The cells and product are particularly delicate. The cells need a substrate, S, that is extremely expensive. However we can make substrate S from a cheap reactant, A, and a slightly expensive reactant, B. At the end of the exam is a diagram of the whole system. Species A is continously fed into a reactor (Caf 1 mol/ vo 2 L/ s) initilly charged with species...
Please summarise the following content:(no word
limit)
EXPERIMENTAL PROCEDURES Animals All animals including pregnant ICR mice, neonatal Wistar rats, and NOD-SCID mice (8 weeks old, male) were purchased from CLEA Japan (Tokyo). All exper- imental procedures and protocols were approved by the Animal Care and Use Committee of Keio University and conformed to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Cells Mouse ESCs were obtained from the Laboratory of Pluripotent Cell Studies, RIKEN...
reply ma as soon as possible
120 points total Due: October 23, 2019 Budget your time carefully TRUE OR FALSE (45 points - 3 points cach): Mark whether the statements as written are "true" or "false". If the statement is false, make minor changes to the statement so that the statement is true. The primary adverse health effect resulting from the disposal of mercury wastes into Minamata Bay was dysentery The flow regime in horizontal velocity grit chambers approaches continuous...
explain figure 2 please on the third page
The Jounal of Immunol nes of the National Academy of Sciences USA 89. 6550-6554. 01992, by permision of Proc. Natl. Acad. Sci. USA Vol. 89, pp. 6550-63554, July 1992 Immunolog A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells RANDOLPH J. NOELLE时, MEENAKSHI ROY.. DAVID M. SHEPHERD., IVAN STAMENKOVICt JEFFREY A. LEDBETTER, AND ALEJANDRO ARUFFo Departmend Microbiology. Dartmouth Medical School One...
please help me. answer only first page. thank you!
p. If you accidentally forgot to inoculate your organism in an OF-glucose tube with oil, and only had one yellow “no oil” tube, what type of carbohydrate catabolism would describe that organism (fermenter, oxidative, neither)? Explain your answer. q. What are two other results from the OF-glucose tubes other than their type of carbohydrate catabolism? r. How can organisms that don't use starch grow on a starch agar plate? Carbohydrate Catabolism...
LAR STUDY GUM Exercises 76, 77, 78, 79,719, 7.11.8.19.2.0.7.0 1. Define niche A ecological envi B by-product of microbial metabolic activities C icorial growth D host-preste relationship E None of the above 2.Microbes muy compete ayust the environment tempera requirements in order to compete for nutrients A True B. False , c.) to the prima A B The pathogenesis of disease can move son of issues by extracellule enzymes or physical blocking of capillaries due to unlimited microbial greneth True...
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