How would the results of the starch test run in the lab be different if the...
How would the results of the starch test run in the lab be different if the organism was only capable of producing amylase, but amylopectin was the only form of starch in the media.
Homework Answers
Starch normally has amylose and amylopectin units. Amylose is straight chain of glucose molecules with 1-4 linkages. Amylopectin is highly branched molecule with lot of 1-6 glycosidic linkages. Amylase can break 1-4 linkages. Where as it is incapable of breaking 1-6 linkages.
If in the lab there is only amylopectin, the amylase will not be able digest it. So, when we do starch test after few minutes of adding amylase, still we see that the starch test gives positive results. This is because amylase is incapable of breaking 1-6 linkages in the amylopectin molecule.
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How would the results of the starch test run in the lab be
different if the...
LAB TOPIC 4 Enzymes 99 TABLE 4.6 Time of Starch Disappearance in Different Concentrations of the...
LAB TOPIC 4 Enzymes 99 TABLE 4.6 Time of Starch Disappearance in Different Concentrations of the Enzyme Amylase Download an Excel version from www.masteringbiology.com in ther Study Area under Lab Media Time of Starch Disappearance (in seconds) Tube % Amylase 0.50 0.25 2 0.125 0.063 0.031 2. Construct a graph (Figure 4.4) to illustrate your results. See Lab Topic 1 for assistance in graph construction. a. What is the independent variable? Which is the appropriate axis variable? for this FIGURE...
please complete table (You can refer to the complete Lab Procedure which is posted separately for...
please complete table
(You can refer to the complete Lab Procedure which is posted separately for more information 1: Characterization of carbohydrates with and without digestion by You are given a set of samples: starch, sucrose, and glucose. The samples are tested before and 37°C water bath for 10 minutes. This temperature is the normal temperature of the human body. after different enzymes are added. After the enzymes are added, the samples are incubated in a Based on your understanding...
Iodine reacts with starch to form a blue-colored complex. Explain the results of the iodine test...
Iodine reacts with starch to form a blue-colored complex. Explain the results of the iodine test with the hydrolyzed starch solution.
. HYDROLYSIS WITH ACID (Results AFTER HEATING) Test tube starch + 10%HCIstarch + H20 (control) su...
. HYDROLYSIS WITH ACID (Results AFTER HEATING) Test tube starch + 10%HCIstarch + H20 (control) sucrose+10%HCI Sucrose+ H20 (control) 11%HCl Test tube 2: Test tube 3: Test tube 4: l starch t HO(controll l sucrose + lodine Test Observations: _urnyClicu licu e ? lodine Test Conclusions polysaccharide or not polysaccharide Benedict's Test Observations: Br icv Enc Red Blut Red Blue Benedict's Test Conclusions: reducing sugar present or no reducing sugar present Raducn ar a resen Sugar ga Sugar Students will...
. HYDROLYSIS WITH ACID (Results AFTER HEATING) Test tube starch + 10%HCIstarch + H20 (control) su...
. HYDROLYSIS WITH ACID (Results AFTER HEATING) Test tube starch + 10%HCIstarch + H20 (control) sucrose+10%HCI Sucrose+ H20 (control) 11%HCl Test tube 2: Test tube 3: Test tube 4: l starch t HO(controll l sucrose + lodine Test Observations: _urnyClicu licu e ? lodine Test Conclusions polysaccharide or not polysaccharide Benedict's Test Observations: Br icv Enc Red Blut Red Blue Benedict's Test Conclusions: reducing sugar present or no reducing sugar present Raducn ar a resen Sugar ga Sugar Students will...
Can the iodine test distinguish between amylose and amylopectin? What Would you look for? Can sucrose...
Can the iodine test distinguish between amylose and amylopectin? What Would you look for? Can sucrose act as a reducing sugar? Explain your answer. In the acid-catalyzed hydrolysis of starch, how can you tell when the hydrolysis of starch is complete? Why does the test work this way? What is the monosaccharide that results at the end? Polysaccharides generally do not give a positive test with Fehling's solution. Why?
When measuring your starch samples in a spectrophotometer, what sort of results would you expect to...
When measuring your starch samples in a spectrophotometer, what sort of results would you expect to see if there were: a. different concentrations of starch in two tubes? b. How could you tell if there was the same concentrations of starch in two tubes? c. How could you tell if there was no starch in a tube?
Name l_ent 11 Lab Report-ENZYMES: HYDROLYSIS OF STARCH BY AMYLASE Partner Section l113 Station # ...
Name l_ent 11 Lab Report-ENZYMES: HYDROLYSIS OF STARCH BY AMYLASE Partner Section l113 Station # PART B. Hydrolysis of starch by increasing amounts of extract Table 1. Absorbance at 590 nm after 5 min incubation of starcn % of starch remaining ps of | Absorbance at 590 | oextract nm after incubation after 5 min incubation 100 0 .312 laa 3 3 5 absorbance of tube O (no enzyme) reflects the total amount of starch initially nt, so the %...
61. How many different test results can you get from 1 SIM test? a. 1 b....
61. How many different test results can you get from 1 SIM test? a. 1 b. 2 c. 3 d. 4 62. How can SIM medium be used to assess motility? a. Growth radiates outward in all directions from the stab line and on the surface b. Media turns black c. Formation of a pink color on the surface of the agar d. More than 1 of the above 63. What type of respiration results in the production of H2S...
please fill out chart and answer applicable questions attached for thumbs up Part 1: Characterization of...
please fill out chart and answer applicable questions attached
for thumbs up
Part 1: Characterization of carbohydrates with and without digestion by enzymes (You can refer to the complete Lab Procedure which is posted separately for more information about this part of the lab.) You are given a set of samples: starch, sucrose, and glucose. The samples are tested before and after different enzymes are added. After the enzymes are added, the samples are incubated in a 37°C water bath...
LAB TOPIC 4 Enzymes 99 TABLE 4.6 Time of Starch Disappearance in Different Concentrations of the Enzyme Amylase Download an Excel version from www.masteringbiology.com in ther Study Area under Lab Media Time of Starch Disappearance (in seconds) Tube % Amylase 0.50 0.25 2 0.125 0.063 0.031 2. Construct a graph (Figure 4.4) to illustrate your results. See Lab Topic 1 for assistance in graph construction. a. What is the independent variable? Which is the appropriate axis variable? for this FIGURE...
please complete table
(You can refer to the complete Lab Procedure which is posted separately for more information 1: Characterization of carbohydrates with and without digestion by You are given a set of samples: starch, sucrose, and glucose. The samples are tested before and 37°C water bath for 10 minutes. This temperature is the normal temperature of the human body. after different enzymes are added. After the enzymes are added, the samples are incubated in a Based on your understanding...
Iodine reacts with starch to form a blue-colored complex. Explain the results of the iodine test with the hydrolyzed starch solution.
. HYDROLYSIS WITH ACID (Results AFTER HEATING) Test tube starch + 10%HCIstarch + H20 (control) sucrose+10%HCI Sucrose+ H20 (control) 11%HCl Test tube 2: Test tube 3: Test tube 4: l starch t HO(controll l sucrose + lodine Test Observations: _urnyClicu licu e ? lodine Test Conclusions polysaccharide or not polysaccharide Benedict's Test Observations: Br icv Enc Red Blut Red Blue Benedict's Test Conclusions: reducing sugar present or no reducing sugar present Raducn ar a resen Sugar ga Sugar Students will...
. HYDROLYSIS WITH ACID (Results AFTER HEATING) Test tube starch + 10%HCIstarch + H20 (control) sucrose+10%HCI Sucrose+ H20 (control) 11%HCl Test tube 2: Test tube 3: Test tube 4: l starch t HO(controll l sucrose + lodine Test Observations: _urnyClicu licu e ? lodine Test Conclusions polysaccharide or not polysaccharide Benedict's Test Observations: Br icv Enc Red Blut Red Blue Benedict's Test Conclusions: reducing sugar present or no reducing sugar present Raducn ar a resen Sugar ga Sugar Students will...
Can the iodine test distinguish between amylose and amylopectin? What Would you look for? Can sucrose act as a reducing sugar? Explain your answer. In the acid-catalyzed hydrolysis of starch, how can you tell when the hydrolysis of starch is complete? Why does the test work this way? What is the monosaccharide that results at the end? Polysaccharides generally do not give a positive test with Fehling's solution. Why?
Name l_ent 11 Lab Report-ENZYMES: HYDROLYSIS OF STARCH BY AMYLASE Partner Section l113 Station # PART B. Hydrolysis of starch by increasing amounts of extract Table 1. Absorbance at 590 nm after 5 min incubation of starcn % of starch remaining ps of | Absorbance at 590 | oextract nm after incubation after 5 min incubation 100 0 .312 laa 3 3 5 absorbance of tube O (no enzyme) reflects the total amount of starch initially nt, so the %...
61. How many different test results can you get from 1 SIM test? a. 1 b. 2 c. 3 d. 4 62. How can SIM medium be used to assess motility? a. Growth radiates outward in all directions from the stab line and on the surface b. Media turns black c. Formation of a pink color on the surface of the agar d. More than 1 of the above 63. What type of respiration results in the production of H2S...
please fill out chart and answer applicable questions attached
for thumbs up
Part 1: Characterization of carbohydrates with and without digestion by enzymes (You can refer to the complete Lab Procedure which is posted separately for more information about this part of the lab.) You are given a set of samples: starch, sucrose, and glucose. The samples are tested before and after different enzymes are added. After the enzymes are added, the samples are incubated in a 37°C water bath...
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