Question

1. Human lysosomes contain hydrolytic enzymes that are activated at a pH of 4.5-5.0. Which of the following factors could alter the structure integrity of a hydrolytic enyzme causing a loss of function? (MULITPLE ANSWERS – select all that apply)

a. temperature below body temperature

b. pH above 5.0

c. pH below 4.5

d. temerature above body temperature

e. genetic mutation in hydrolytic enzyme gene

Answer 1

As the enzyme is best suited to work in the pH range of 4.5-5.0, if the pH is above 5.0 or when below 4.5, it would decrease its activity. This would however not cause loss of function.

Temperature below bdy temperature would also reduce the catalytic activity.

A genetic mutation can render the enzyme functionless. Increase in temperature can denature the enzyme, thus abolidhing its function. Thus, options d and e are right.