Question

Question 14 Which of the following is a feature common to BOTH prokaryotes and eukaryotes? The use of nucleosomes to condense DNA in the nucleus. The ability to translate an RNA before its transcription is complete. The ability to have multiple ribosomes on a single RNA for more efficient translation. The ability to start transcription at a 5'AUG sequence. o Question 15 A particular prokaryotic promoter contains only the region from-10 to-35. Which of the following is true? The RNA polymerase holoenzyme will start transcription downstream of this promoter. The RNA polymerase holoenzyme will start transcription upstream of this promoter. This promoter contains the start site of transcription for the RNA polymerase holoenzyme. This entire promoter will be transcribed if the RNA polymerase holoenzyme is present. Question 16 Which of the following does NOT typically occur at the RNA polymerase II CTD? Binding to poly-A polymerase O Phosphorylation by TFIIH. O Binding to the spliceosome. O Dephosphorylation. Question 17 You have discovered a poison that stops the activity of guanylyltransferases. What should be observed in eukaryotic cells treated with high concentrations of this poison? O mRNAs with two phosphate groups at their 5'ends. O mRNAs that are more resistant to exonucleases. OmRNAs with three phosphate groups at their 5'ends. mRNAs that will be translated, but will be more quickly degraded.

Answer 1

14.Common feature of both prokaryotes and eukaryotes is :- ability to have multiple ribosome on a single mRNA for more efficient translation. This structure also known as Polyribosome or polysome.

15.If -35 bp and - 10 bp consensus sequence present In prokaryotic then :- RNA polymerase holoenzyme will start transcription downstream of this promoter.

16.Binding to Poly A polymerase not occurs at CDT tail of RNA polymerase II. CTD tail of RNA pol II is a extension of large subunit, is involve phosphorylation by TFIIH transcription factors. CTD tail also involved in binding of spliceosome

17.During eukaryotic capping, a phosphohydrolase enzyme remove gamma phosphate from 5' nascent mRNA and creating 5'diphosphate .The Guanylyl transferase enzyme added Guanine residue at the 5' end of mRNA. Now if this poison block this enzyme then mRNA will have two phsophate group at the 5' end.