Question

order these methods from what you will start first and end for studying a specific virus:

a. flow cytometry

b. western blot

c. cell culture system

d. microscopy

e. PCR

Answer 1

Ans: The order in which the methods are to be followed for studying a specific virus:

c. Cell culture system > d. Microscopy > e. PCR > b. Western blot > a. Flow Cytometry

First, the primary cell cultures are generated in order to increase the cell number.

Second, visualization of virus cells using microscopy techniques.

Third, the quantification of the viral genome using PCR techniques. It measures the number of virus cells present in a sample.

Fourth, To confirm the identity of reactive antigen Western blot technique is carried out by SDS-PAGE.

Fifth, flow cytometry is molecular and biochemical technique used to investigate virology. this is used to get pure population of cells from a mixture. hence is the part for viral diagnostics.