Question
Microbiology

Q1
A Durham tube is commonly added to the lactose or glucose broth to test for the release of Acid gas Both acid and gas
Q2
SIM stands for sulfur, indole, and methyl red. True False
Q3
The citrate slant is a differential medium. True False
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Answer #1

Answer 1:
Option - gas

In lactose or glucose broth, inverted Durham's tube is placed to catch any gas released during the fermentation of sugars. Air bubble in the tube is indicative of gas. Acid production is observed by change in the color of the medium to yellow due to phenol red indicator.

Answer 2: False

SIM agar stands for Sulphur, Indole, Motility.

Answer 3: True

Citrate differentiates between organisms that can utililize sodium citrate as sole source of carbon and energy from those who cannot.

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