Possible cause | Recommendation |
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Antibiotic-resistant strain |
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Vector plasmid recombined into the host chromosome |
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Satellite colonies |
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High number of cells plated |
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Cell contamination |
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2) some essential tips to maintain an aseptic environment and prevent cell culture contamination.
If I performed a transformation, how do I prevent growth that have not been transformed? if...
12a. If you plated transformed cells on LB, what would the growth look like? Why? b. How else could growth occur on plates with both antibiotics with regard to “successful” transformation? (Hint: How else could you both genes “get into” bacterial cells?) c. What other ways can growth occur on plates with both antibiotics () that do not have anything to do with transformation/molecular cloning?
how do you prevent VTE? What should the patient do? what should they avoid?
Antioxidants (A) have been shown in some studies to promote cancer cell growth (B) have been proven to prevent cancer (C) are found naturally in fruits and vegetables (D) a, b and c (E) a and c only
How do I calculate the number of cells recovered in a suspension? What information do I need? Also, calculate the percent increase in cell density from the time of IPTG addition to the time when the cells were harvested. The OD of the E.coli culture is around OD 2-2.5. 10ml of culture was added to each flask. Components IPTG (+) OD600 IPTG(-) OD600 E.coli+Glucose+Ampicillin 0.6263 0.5764 E.coli+Glucose+Ampicillin after IPTG and growth 0.2870 0.2041
We performed an experiment to find bacteriophage growth/ burst size. Nobody got good results that make sense, and our teacher asked us this. "The experiment we performed to determine the average number of mature virions released did not work. We tried to syringe filter the virus before we infected the bacteria and then plated. Think about how we performed this experiment and offer an explanation as to why the experiment may not have worked." I don't understand what went wrong....
Culture of Yeast that requires uracil for growth (ura3-) Two mutants that are ura3- have been isolated identified as X and Y Denotes growth of cells ura3 ura3 These mutants or the Diploid fusion (Diploid X- Y) were plated on growth media What can be deduced from Diploid X the data to the right? Diploid X-Y Growth at 23 °C Growth at 32°C
You have a data set C1 and you do the following transformation using statistical software: C2=C1/5 You find the mean, median and standard deviation of the original C1 and the new C2 and compare them. You must then answer the question pictured below. I assumed "none of them" would be the answer since all three values changed after the transformation, but that answer if wrong. •If you shift the original data (C1) as follows: C3=C1+5 how would you answer the...
A transformation experiment is performed with a donor strain of M. falcieri that is resistant to four drugs: A, B, C, and D. The recipient is sensitive to all four drugs. The transformed recipient cells are split in aliquots and plated on media containing various combinations of the drugs. The results are below. All drugs are added at the same time. Drug(s) added # of colonies Drug(s) added # of colonies None 10,000 BC 50 A 1156 BD 48 B...
how do i assess a company's its future sales growth? how do i assess a company's future level of profitability and profit retention?
1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation occurred on which agar plate (s)? What evidence do you have that the bacteria were transformed here? 3. Which plates have glowing growth? Explain what causes bacteria to glow. II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...