1.What does CRISPR stand for?
2.What is CRISPR technology?
3.What does this technology have the potential to offer society?
4.Why is CRISPR (and gene editing) important? What are the ethical issues with CRISPR?
5.What are some potential drawbacks of CRISPR technology?
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1.What does CRISPR stand for? 2.What is CRISPR technology? 3.What does this technology have the potential...
Question 1: What was determined to be the function of the CRISPR loci in microbes? Question 2: Explain the differences in the roles of the cas7 and cas9 gene. Question 3: What genetic material does CRISPR target? Question 4: Due to the ability of CRISPR to cleave DNA sequences at specific sites, it is considered a programmable version of what? Question 5: Define and explain the significance of the PAM sequence. Question 6: What is the role of tracrRNA in...
1. What is the source of the “spacer” DNA in the bacterial CRISPR locus? 2. Why does the cas9 enzyme have two nuclease domains? 3. What is the relationship between the tracrRNA and the regularly interspersed repeats of the CRISPR locus? Why is this important? 4. In the lab version of the CRISPR/cas9 system, what is the “chimeric RNA”?
1. what is CRISPR gene editing? 2. what enzyme is used to cut DNA? 3.Why do bacteria use CRISPR? 4.How is the enzyme targeted to a specific DNA sequence? 5.what need to be done in order to make sure the proper human protein is made in bacteria cells? 6.How can you check for proper orientation of the inserted DNA in the plasmid? 7. Why are iPSCs useful for studying hereditary neuronal diseases such as Alzheimer's. 8. How are pig cells...
A number of advances have been made in biotechnology. CRISPR/Cas9 one of the most controversial, and is getting a lot of current media attention. It is a method by which scientists can precisely edit DNA sequences at exact locations. Benefits obviously include the potential to “repair” mutated genes that cause disease. In fact, preliminary results from one of the earliest clinical trials of CRISPR/Cas9 provide evidence that the technique is safe and feasible to use for treating human diseases. What...
please help answer these questions 3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based gene editing versus Zinc-finger nucleases and TALENS? 4. What is crRNA and what does it do? 5. What is tracrRNA and what does it do? 6. What is the PAM sequence and what is its significance? 7. What can nuclease-deficient Cas9 (acas) proteins be used for? 8. You want to insert DNA encoding an epitope tag to the end of a specific gene you...
1: CRISPR technology is used to alter gene expression. i) In addition to a target DNA sequence, what 3 components are required to alter a gene using CRISPR technology? Answer using complete sentences for full credit. (3 points extra) ii) All 3 key components of CRISPR technology described in 1 (i) are macromolecular polymers. Explain what monomeric units compose each of the 3 components. Answer using complete sentences for full credit. (3 points extra)
what are the implications of the Crispr-Cas technology in: 1) Evolution 2) Medicine 3) Conservation Ecology ?
We have been reading about genetic "engineering" and CRISPR-type technology. 1. What is the biggest advantage that you can see with technology such as this? 2. What is the biggest "scare factor" or disadvantage that you can see? 3. How does your STEWARDSHIP impact future trends in health care management?
1. What is the purpose of the thermal compound 2. What does NIC stand for? Why is it important to have a NIC in a modern computer?
Topic 1 [video]: Jennifer Doudna on CRISPR. View the NBC News story about CRISPR (1)*, including an interview with its co-discoverer, Jennifer Doudna. Then, address the following questions: (a) What applications of CRISPR look particularly promising for improving human well-being? (b) What sort of ethical issues have arisen or are likely to arise with the deployment of CRISPR?