Describe a simple transgenic plant you want to make (any) and its gene function (eg/ a...
Describe a simple transgenic plant you want to make (any) and its gene function (eg/ a gene that encodes a specific enzyme)
Homework Answers
Answer: Stearidonic acid rich soybean plant is a transgenic plant that contain high content of nutritionally superior fatty acid called as stearidonic acid. In general, soybean does not contain stearidonic acid. However, it contain alpha-linolenic acid which is the precursor for the stearidonic acid. An enzyme called as Delta-6-Desaturase catalyzes the conversion of alpha-linolenic acid to stearidonic acid.
Transgenic stearidonic acid rich soybean plant is generated by transgenic expression of Delta-6-Desaturase gene from the algae. Transformed plants with this gene produces an enzyme called as Delta-6-Desaturase that catalyzes the conversion of alpha-linolenic acid to stearidonic acid.
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Describe a simple transgenic plant you want to make (any) and
its gene function (eg/ a...
A transgenic plant was constructed by introducing a fungal gene via Agrobacterium mediated transformation. The gene...
A transgenic plant was constructed by introducing a fungal gene via Agrobacterium mediated transformation. The gene was isolated from the fungus Aspergillus niger and encodes for biosynthesis of a metabolite that enhances plant growth. The gene is not present in wild type plants. However, the transgenic plants do not have the expected phenotypes. How are you going to 60 demonstrate that the fungus gene is present in those plants, despite the lack of a phenotype?
Suppose you are working on a gene that encodes a protein that triggers apoptosis of the...
Suppose you are working on a gene that encodes a protein that triggers apoptosis of the cell (programmed cell death). In a certain cell, this gene is transcriptionally ON, that is, primary transcript is being made. Choose a mechanism by which to prevent or reduce the production of active apoptosis protein, and describe the specific properties of the new regulatory protein that the cell would have to make in order to do this.
6a) You want to use homologous recombination to generate a mouse that does not express a functional XPC gene (‘Knock-out...
6a) You want to use homologous recombination to generate a mouse that does not express a functional XPC gene (‘Knock-out’ or KO). To do this, you want to delete exon 10 and replace it with a gene that confers resistance to the drug Neomycin (NEO. The usual second selection with tk we discussed in class is not shown here). Using the diagram as a guide: illustrate on the diagram where you expect crossing over to occur and (below the diagram)...
7) Describe the hedgehog gene.. Be sure to explain its' function and its region of activity...
7) Describe the hedgehog gene.. Be sure to explain its' function and its region of activity in the body. 8) Describe the fossil Tiktaalik. Why does this fossil confirm a major prediction of paleontology?..
Find information (you may want to search the internet) allowing you to describe a specific example...
Find information (you may want to search the internet) allowing you to describe a specific example of an extracellular signal that acts through a G protein. Give details about the pathway and make sure to name the original signal molecule and describe the change in cell function. (12 points)
A. You want to make a translational fusion of protein Z and the yellow fluorescent protein...
A. You want to make a translational fusion of protein Z and the yellow fluorescent protein YFP (Z-YFP) so that expression of the fusion occurs with the same cell-specific pattern as that of protein Z. The protein Z gene has a 2 kb promoter, two 1 kb exons, and one 5 kb intron. The YFP gene is 0.7 kb. Outline with words and sketches your strategy to make the Z-YFP fusion clone using PCR. You already have cells that contain...
Plants: You isolate a plant with a loss-of- function mutation in the HJK gene. hjk genes...
Plants: You isolate a plant with a loss-of- function mutation in the HJK gene. hjk genes don't elongate properly and have cells with abnormal phases of cell division. You show that the wild type HJK protein is required for proper orientation of cortical microtubules. Explain why hjk mutants display their mutant phenotypes
Please answer 2-5 2. Consider a gene with a particular function. Mutation X and mutation Y...
Please answer 2-5
2. Consider a gene with a particular function. Mutation X and mutation Y cach cause defects in the function of the encoded protein, yet a gene containing both mutations X and Y encodes a protein that works even better than the original protein. The odds are exceedingly small that a single mutational event will generate both mutations X and Y. Explain a simple way that an organism with a mutant gene containing both mutations X and Y...
Describe in one paragraph which gene you think is mutated and why, based on the information...
Describe in one paragraph which gene you think is mutated and why, based on the information in Flybase. Include the name of the gene, its function and your rationale for why you believe it is mutated in your flies? (The gene dmel/hsf is mutated because the mutated alleles exhibits compromised thermotolerance)
You are studying the regulatory DNA of a mouse gene expressed in developing heart, liver, and...
You are studying the regulatory DNA of a mouse gene expressed in developing heart, liver, and lung tissue. Your preliminary work has shown that heart and lung expression of this gene is controlled by a short fragment of DNA just upstream of the promoter. Based on this result, you decide to investigate this region further to understand its function. You decide to compare this sequence to the regulatory DNA of the same gene found in rats and humans. Using genome...
7) Describe the hedgehog gene.. Be sure to explain its' function and its region of activity in the body. 8) Describe the fossil Tiktaalik. Why does this fossil confirm a major prediction of paleontology?..
Find information (you may want to search the internet) allowing you to describe a specific example of an extracellular signal that acts through a G protein. Give details about the pathway and make sure to name the original signal molecule and describe the change in cell function. (12 points)
Please answer 2-5
2. Consider a gene with a particular function. Mutation X and mutation Y cach cause defects in the function of the encoded protein, yet a gene containing both mutations X and Y encodes a protein that works even better than the original protein. The odds are exceedingly small that a single mutational event will generate both mutations X and Y. Explain a simple way that an organism with a mutant gene containing both mutations X and Y...
You are studying the regulatory DNA of a mouse gene expressed in developing heart, liver, and lung tissue. Your preliminary work has shown that heart and lung expression of this gene is controlled by a short fragment of DNA just upstream of the promoter. Based on this result, you decide to investigate this region further to understand its function. You decide to compare this sequence to the regulatory DNA of the same gene found in rats and humans. Using genome...
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