What happens to DNA synthesis when a dideoxyribonucleoside triphosphate (ddNTP) is added to a growing DNA...
1) What happens to DNA synthesis when a dideoxyribonucleoside triphosphate ( ddNTP) is added to a growing DNA chain ( rather than the normal deoxyribonucleoside triphosphate) ? 2) About how many copies of DNA are made after 10 cycle of PCR ? 3) What are we doing when we amplify a particular DNA segment ? 4) How are DNA fragments stored in a cDNA library ? 5) What is the basic purpose of a DNA microarray ? Please answer all...
14.7 A number of antiviral drugs act by preventing viral DNA synthesis. These drugs are typically chemically similar to nucleotides with a modification. For example, the drug may contain all relevant components of a nucleotide, including a proper purine or pyrimidine base, but lack the 3' OH group. How would this modification allow this drug to prevent viral DNA synthesis? A. Once inserted into a growing DNA chain, the drug will not be able to form a normal double-helix, as...
Someone answered 3'OH, Ribonucleotide, Triphosphate and Ribonucleotide respectively, but one is incorrect, I dont know which one. Please help. The nucleotide shown here is called cordycepin triphosphate. It is a natural product of a fungus that is used in traditional medicines. P-P-P 5' Base он If cordycepin triphosphate is added to a cell-free transcription reaction, the nucleotide is added onto the growing RNA chain but then no more nucleotides can be added. Examine the structure of cordycepin and explain why...
Some DNA polymerases have proofreading activities. After a nucleotide is added to a growing nucleic acid chain, it can be removed if it incorrectly pairs with the template. Speculate if ribosomes have similar proofreading activities during translation and give your reason.
P-P-P-O-CH2 deoxycytidine triphosphate (dCTP) OH H P-P-P-O-CH2 dideoxycytidine triphosphate (ddCTP) NH2 P -0-CH dideoxycytidine monophosphate (ddCMP) HH A. What would you expect if ddCTP were added to a DNA replication reaction in large excess over the concentration of the available CTP, the normal deoxycytidine triphosphate? B. What would happen if it were added at 10% of the concentration of the available dCTP? C. What effects would you expect if ddCMP were added under the same conditions?
a) What happens when LiOH is added to a buffer composed of HC2H3O2 and C2H3O2 - ? Write a chemical equation for that reaction. b) What happens when HBr is added to this buffer? Write a chemical equation for that reaction.
If helicase is not added to a reaction mixture but DNA synthesis still occurred. How is this possible?
Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? The complete DNA strands are synthesized then sequenced. Numerous synthesized fragments of DNA are sequenced to determine which nucleotides were incorporated. The sequencing occurs during S phase of the cell cycle. RNA molecules are synthesized off of the DNA templates and the incorporated nucleotides are then determined. Incorporated nucleotides are determined while they are being added to a growing DNA strand.
what happens to resistance of a series when light bulbs are added to the series? why what happens to the overall resistance of a parellel circuit when light bulb are added? why?
When setting up the polymerase chain reaction, approximately 200 ng of DNA should be added in a 50 ul reaction. How many ul of the above (it was 115 ng/ul) undiluted DNA solution should be added to the PCR tube