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• 1. This medium is agar. A 2. A positive test would indicate that end products...
Background Background continued... Questions Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram...
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Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
Microbiology Q1 Q2 Q3 What is the color of SIM medium that contains an organism that...
Microbiology
Q1
Q2
Q3
What is the color of SIM medium that contains an organism that forms hydrogen sulfide (is positive for sulfur)? Blue Black Red Yellow Which test(s) would you use to find out more about Gram-positive organisms? SIM MR Citrate None of the tests listed. Fermentation of sugars usually results in end products. Acidic Basic (Alkaline)
help please On a mannitol-salt agar plate, a bacterium grows on the plate, and turns medium...
help please
On a mannitol-salt agar plate, a bacterium grows on the plate, and turns medium color from light orange to bright yellow. The color indicator is phenol red. This bacterium must be capable of: Select one: O a. Fermenting lactose to make acidic by-products. O b. Utilizing citrate as a main carbohydrate source to grow. O c. Fermenting glucose to make acidic products, which lowers the pH of the MSA agar. O d. Fermenting mannitol to make acidic products,...
Medium/test used for/ determines indicator / key ingredients positive result negative result Comments/ additional info quadrant...
Medium/test used for/ determines indicator / key ingredients positive result negative result Comments/ additional info quadrant streak Gram stain motility agar Eosin methylene blue agar (EMB) SIM Nm multi test medium (3 results in one medium) Brewer's plate in GasPak Fluid thioglycollate (FTM) Citrate slant carbohydrate test media (list the ones tested) phenylalanine agar urea agar or broth MR/VP broth MR test: VP test: nitrate broth catalase test oxidase test casein agar gelatin deep lysine iron Pseudo. F agar &...
2. Enzymes that we are testing for would be produced by the that is being catabolized...
2. Enzymes that we are testing for would be produced by the that is being catabolized is considered a while the amino acid material or ingredient. 3. Indicate, with arrows, where each of the following enzymes breaks a bond in the amino acid. Phenylalanine Deaminase Lysine Decarboxylase Tryptophanase Cysteine Desulfhydrase 4. Kovac's reagent is added to the medium and will form a ring at the top of the media if the result is positive. The enzyme that may be produced...
Can anyone help me by checking my answers? please indicate the answer in a numerical order....
Can anyone help me by checking my answers? please indicate the
answer in a numerical order. thank you.
Question 1 1 pts TRUE or FALSE: When we are working with our metabolic unknown samples, we should prepare a new TSA slant each day. True False Question 2 2 pts Which of the following is NOT a possible result of a test in which your unknown bacteria were introduced into a tube of phenol red lactose broth? Fermentation of lactose producing...
1. Someone new is preparing media for lab and adds buffers to the EMB agar that...
1. Someone new is preparing media for lab and adds buffers to the EMB agar that raise the pH to 7.8. The starting pH of EMB plates should be 6.9 – 7.3. No one is aware of the change. The EMB plates are inoculated for identification of unknown bacteria. After the appropriate incubation time, the results are interpreted. A. Discuss the consequences of the change in starting pH to the interpretation of the results. (The answer is not that no...
Use nunber 26 to answer A please give me only short answer not long answer please...
Use nunber 26 to answer A please give me only short answer not long
answer please
You've been asked to USE a bacteriological medium (agar) that is BOTH complex AND differential so that you can isolate organisms that can ferment sucrose to acidic end products. Look at the two recipes below: Medium 1 contains: water, agar, sucrose (C12H20,.), glutamine (CsHioN,O,), penicillin (CstH&N,0.S), KH,PO yeast extract, and phenol red dye (CisHsuo,S, at pH <6 this dye turns the agar yellow, at...
B. Reaction mechanisms (media, reagents, enzymes, products) 1. Media. Match the name of medium with the...
B. Reaction mechanisms (media, reagents, enzymes, products) 1. Media. Match the name of medium with the physiological test. For some tests, multiple media can be used. a. Kligler's iron agar b. MR-VP broth c. Bromocresol Purple (BCP) lactose d. SIM medium e. Simmon's citrate agar f. Skim milk agar 8. Spirit Blue agar h. Tryptone broth _2,3 butanediol fermentation carbohydrate fermentation _casein hydrolysis citrate utilization hydrogen sulfide production mixed acid fermentation triglyceride hydrolysis _tryptophan degradation
B. Citrate Test. • This is a test for citrate utilization. Identifies bacteria capable of using...
B. Citrate Test. • This is a test for citrate utilization. Identifies bacteria capable of using citrate as a sole carbon source. • These bacteria produce the enzyme Citrate Permease that works in conjunction with Citrate Lyase to produce the end products • Utilization of citrate involves the breakdown of citrate to oxaloacetate and acetate. Oxaloacetate is in turn broken down to Pyruvate and CO, Utilization of ammonium salts in the medium produces ammonium hydroxide that renders the medium alkaline...
Background
Background continued...
Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
Microbiology
Q1
Q2
Q3
What is the color of SIM medium that contains an organism that forms hydrogen sulfide (is positive for sulfur)? Blue Black Red Yellow Which test(s) would you use to find out more about Gram-positive organisms? SIM MR Citrate None of the tests listed. Fermentation of sugars usually results in end products. Acidic Basic (Alkaline)
help please
On a mannitol-salt agar plate, a bacterium grows on the plate, and turns medium color from light orange to bright yellow. The color indicator is phenol red. This bacterium must be capable of: Select one: O a. Fermenting lactose to make acidic by-products. O b. Utilizing citrate as a main carbohydrate source to grow. O c. Fermenting glucose to make acidic products, which lowers the pH of the MSA agar. O d. Fermenting mannitol to make acidic products,...
Medium/test used for/ determines indicator / key ingredients positive result negative result Comments/ additional info quadrant streak Gram stain motility agar Eosin methylene blue agar (EMB) SIM Nm multi test medium (3 results in one medium) Brewer's plate in GasPak Fluid thioglycollate (FTM) Citrate slant carbohydrate test media (list the ones tested) phenylalanine agar urea agar or broth MR/VP broth MR test: VP test: nitrate broth catalase test oxidase test casein agar gelatin deep lysine iron Pseudo. F agar &...
2. Enzymes that we are testing for would be produced by the that is being catabolized is considered a while the amino acid material or ingredient. 3. Indicate, with arrows, where each of the following enzymes breaks a bond in the amino acid. Phenylalanine Deaminase Lysine Decarboxylase Tryptophanase Cysteine Desulfhydrase 4. Kovac's reagent is added to the medium and will form a ring at the top of the media if the result is positive. The enzyme that may be produced...
Can anyone help me by checking my answers? please indicate the
answer in a numerical order. thank you.
Question 1 1 pts TRUE or FALSE: When we are working with our metabolic unknown samples, we should prepare a new TSA slant each day. True False Question 2 2 pts Which of the following is NOT a possible result of a test in which your unknown bacteria were introduced into a tube of phenol red lactose broth? Fermentation of lactose producing...
Use nunber 26 to answer A please give me only short answer not long
answer please
You've been asked to USE a bacteriological medium (agar) that is BOTH complex AND differential so that you can isolate organisms that can ferment sucrose to acidic end products. Look at the two recipes below: Medium 1 contains: water, agar, sucrose (C12H20,.), glutamine (CsHioN,O,), penicillin (CstH&N,0.S), KH,PO yeast extract, and phenol red dye (CisHsuo,S, at pH <6 this dye turns the agar yellow, at...
B. Reaction mechanisms (media, reagents, enzymes, products) 1. Media. Match the name of medium with the physiological test. For some tests, multiple media can be used. a. Kligler's iron agar b. MR-VP broth c. Bromocresol Purple (BCP) lactose d. SIM medium e. Simmon's citrate agar f. Skim milk agar 8. Spirit Blue agar h. Tryptone broth _2,3 butanediol fermentation carbohydrate fermentation _casein hydrolysis citrate utilization hydrogen sulfide production mixed acid fermentation triglyceride hydrolysis _tryptophan degradation
B. Citrate Test. • This is a test for citrate utilization. Identifies bacteria capable of using citrate as a sole carbon source. • These bacteria produce the enzyme Citrate Permease that works in conjunction with Citrate Lyase to produce the end products • Utilization of citrate involves the breakdown of citrate to oxaloacetate and acetate. Oxaloacetate is in turn broken down to Pyruvate and CO, Utilization of ammonium salts in the medium produces ammonium hydroxide that renders the medium alkaline...
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