The function of Na+K+ ATPase in a cell membrane of cultured cells is being investigated. The cell is then supplied with an analog of ATP (ATV, adenosine tri canada the) this binds to the protein but there is no hydrolysis. What would be the affect of this drug on the transporter
# Hydrolysis of ATP is more important for the Na+K+ ATPase transporter. If there is no hydrolysis then, the pump remains active and continues to transport the ions across the membrane. This leads to the abnormal levels of sodium and potassium ions in and out of the cell. This would change the osmolarity of the cell.
The function of Na+K+ ATPase in a cell membrane of cultured cells is being investigated. The...
Question 33 2.5 pts The Na+-K+ ATPase transporter found in the plasma membrane of most animal cells gets its energy from: ATP hydrolysis The electrochemical gradient across the membrane O Co-transport of H+ into the cell None are correct, this transporter functions by facilitated diffusion All are correct
You have isolated the Na/K-ATPase in a semi-purified membrane system. Given higher concentrations of Na' out and higher K inside, addition of ATP to the inside leads to increases of Nat olutside and Kt inside as expected. If instead, you add the ATP to the outside of the membrane, you don't see any change in the ion levels. Why? • ATP acts as an irreversible inhibitor of the Na/K-ATPase if it binds on the exterior side. The active site for...
In considering active transport by Na+-K+-ATPase, 3 Na+ are pumped out of the cell and 2 K+ are pumped in for each ATP that is hydrolyzed to ADP + Pi. Given a ΔG for ATP hydrolysis of -10 kcal/mol, and that V is -60 mV (more electronegative inside), and that the pump typically maintains the internal Na+ at 10mM, external Na+ at 145 mM, internal K+ at 140 mM and external K+ at 5mM, what is the efficiency of the...
1. Animal cells have a Na,K pump that couples the energy of ATP hydrolysis to transport 3 Na ions out of the cell and 2 K ions into the cell. Inside astrocytes, the concentration of Na is 20 mM and the concentration of K is 130 mM. The extracellular concentrations of Na and K are 145 mM and 5 mM, respectively. Calculate the energy required for the transport of Na and K , with this stoichiometry; assume that the cell...
8) The Na+/K+-ATPase enzyme helps cells maintain their membrane potential. For every (1/2/3/4) sodium ions that are (Pumped in/Pumped out), (1/2/3/4) potassium ions are (Pumped in/Pumped out)
1. You have identified a new protein and predict it is a transmembrane protein due to its AA sequence analysis. How would determine where the protein is located in the cell? A. Extract all the proteins from the cell and analyze them on a protein gel. B.Use Polymerase Chain Reaction (PCR) to amplify the protein. C. Use an fluorescent antibody to the protein and allow it to bind to the cells. D Isolate the RNA for the protein and use...
What would most likely happen if the Na+K+ Pump (ATPase) stopped functioning in the cell? A. The cell would swell by taking up water. B. The cell would shrink by pumping out water. C. The inside of the cell would be more negative. D. Nothing would happen to the cell.
Lo 10: Electrochemical Gradient The difference in voltage across the membrane is called the __________________________________. The inside of a normal cell is ___________________ (+/-); while the outside is ___________(+/-). The resting membrane potential of a neuron is ___________mV. Diagram the relative ratios of Ca++, Na+ and K+ in a cell under resting conditions. Draw an arrow for each of the molecules indicating which direction would be passive transport (into or out of the cell). For each of the following sentences, fill in...
1) Glucose enters human enterocytes (intestinal epithelial cells) against its concentration gradient. A Na+ ion enters the cell with each glucose molecule, and while the glucose transporter does not hydrolyze ATP, it depends on a pre-existing gradient of Na+ ions that is created by the Na+/K+ ATPase. This is an example of ____________. A. primary active uniport B. secondary active antiport C. primary active symport D. secondary active symport 2) Which statement is FALSE? A. Acetyl CoA is oxidized to...
During the aerobic metabolism of glucose, glucose is ____________. Reduced to form water Oxidized to form water Reduced to form CO2 Oxidized to form CO2 Which of the following describes the equation: FAD + XH2 à FADH2 + X. FAD is reduced to FADH2 It is a coupled reduction – oxidation reaction XH2 is oxidized to X All of the above Which of the following is FALSE about glycolysis? The initial steps of glycolysis requires energy derived from the splitting...