Question

& Genetic Mutations X 2. Lab12. eScience Transcription X + Chapter 13.2 х /sites/default/files/V3/Biology/2nd_Edition General_Biology/Docs/GB_1713_L12 Transcription_and_Translation/GB_1713_112_Exp2 Mutations.pdf Exercise Genetic Mut Experiment Inventory Materials No materials required EXERCISE 2: GENETIC MUTATIONS 1. Translate the following sequence: AUG GAG GUC UUU AAG AGA CAU VUA GAU GUA GCC CUU AGU GAU GUU UAG 12. Create one or more point mutations in this sequence: AUG GAG GUC UUU AAG AGA CAU VUA GAU GUA GCC CUU AGU GAU GUU UAG 3. Translate your mutated sequence. 14. Now, create a frameshift mutation by adding one or more bases. AUG GAG GUC UUU AAG AGA CAU VUA GAU GUA GCC CUU AGU GAU GUU UAG 15. Translate your mutated sequence. 6. Create a frameshift mutation by deleting one or more bases. AUG GAG GUC UUU AAG AGA CAU UUA GAU GUA GCC CUU AGU GAU GUU UAG 07 7. Translate your mutated sequence. 8. Align the amino acid sequences that you determined for each of the four transcripts.

Need some help with an exercise in my biology course.

Answer 1

1. Met Glu Val Phe Lys Arg His Leu Asp Val Ala Val Ser Asp Val Stop

2. Point mutations: AUG GUG GUC UUU AGG AGA CAU UGA GAU GUA GCC CUU AUU GAU GUU UAG

3. Met Val Val Phe Arg Arg Hist Stop

4. AUG GAC(1) GGU CUU UAA GAG ACA UUU AGG(2) AUG UAG CCC UUA GUG AUG UUU AG

5. Met ASsp Gly Leu Stop

6. AUG GAG GU(1)U UUA AGA GAC AUU UAG AUG UAG CCC UUA GUG AUG UUU AG

7. Met Glu Val Leu Arg Asp Ile Stop

8. Met Glu Val Phe Lys Arg His Leu Asp Val Ala Val Ser Asp Val Stop

Met Val Val Phe Arg Arg Hist Stop

Met ASsp Gly Leu Stop

Met Glu Val Leu Arg Asp Ile Stop