Question

1.) PCR

a.) Using the dsDNA sequence 1 as a template, you want to generate dsDNA sequence 2 using PCR. What

are the sequences of the two primers you use? 15nt of each primer have to anneal perfectly to the template.

Write the primer sequences in 5’->3’ direction in the provided space.

(25 points)

Sequence 1

5-TATAGGACGATGTTGATGAATGGTACAATCACAGTACGTACGTACAGTCAGTGAAA-3

Sequence 2

5-TAGCGGTACATGTTGATGAATGGTACAATCACAGTACGTACGTACAGTCTATCGAT-3

Primer 1 5-

-3

Primer 2 5-

-3

1.) PCR a.) Using the dsDNA sequence 1 as a template, you want to generate dsDNA sequence 2 using PCR. What are the sequences

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Answer #1

Guanive Ca) es Coupin TALGATOT RAe.inte ar Cpimer 1) ance-2 mer-2 e wih G with c

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