You plan to do a proliferation assay using 10 ml of cells that you prepared in A3. You decided to use Concavalin A mitogen to measure the proliferation of T cells using a Flow cytometry proliferation assay. Your stock of Con A stock solution is 100 micrograms/ml. The recommendation is to treat the cells with a concentration of 10ng/ml. What volume of Con A will you add from the stock solution into you 10 ml cells?
stock concentration (M1) = 100 micrograms/mL
concentration needed (M2)= 10 nanogram/mL
final volume (V2)= 10 mL
volume of CoA needed (V1)=?
1 nanogram=10^-3 microgram
M2= 10 10^-3 microgram/mL= 10^-2 microgram/mL
M1V1=M2V2
V1= M2V2/M1
= (10^-210)/100
= 10^-3 mL
1 mL= 1000 microliter
10^-3 mL= 10^31000=1 microliter.
volume of Con A will you add from the stock solution into you 10 ml cells= 1 microliter.
You plan to do a proliferation assay using 10 ml of cells that you prepared in...
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