You want to add Caspase-3 inhibitor to 20 mL of HL-60 cells so that you have a concentration of 50 nanomoles. Your stock solution is 75 micromoles. What is the volume of the stock solution you need?
You want to add Caspase-3 inhibitor to 20 mL of HL-60 cells so that you have...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
You have a stock solution of enzyme (125 kD) at a concentration of 1mg/ml. You want to achieve a final enzyme concentration of 36nM in a 1ml reaction volume. What volume of the enzyme stock solution will you add to the reaction?
You plan to do a proliferation assay using 10 ml of cells that you prepared in A3. You decided to use Concavalin A mitogen to measure the proliferation of T cells using a Flow cytometry proliferation assay. Your stock of Con A stock solution is 100 micrograms/ml. The recommendation is to treat the cells with a concentration of 10ng/ml. What volume of Con A will you add from the stock solution into you 10 ml cells?
You have a stock solution of 25 mg/mL igG and want to make 2000 microliters of protein standard at a concentration of 1.5 mg/mL. What volume of igG stock would you need?
You have a 3 mL suspension of cells at a concentration of 480,000 cells per mL. How much medium (in mL) should you add to the suspension to adjust the concentration to 100,000 cells per mL?
You want to prepare 50 mL of a 20% NaCl salt solution from a 100% stock. Calculate the amount of stock as well as the amount of water you need to use to make this dilution. Assume that you have unlimited volume of the stock.
you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4 cells/mL and need to produce a solution of 56 cells/mL set up detailed serial dilution given 8mL of 4.1 x10^3 cells/ML and need approximitly 100 cells/mL. set up detailed serial dilution by determining the aliquot to be transfered (v1) from the solution to get 100 cells/mL in a final volume of 10 mL given 10mL cell suspension of NIH cells at 3.8 x10^5 cells/ML...
If you have a stock culture of bacteria at a concentration of 5*10 cells/ml, how much stock cuiture and how much media would you add to obtain a final concentration of 2.5*10 cells/ml in a final volume of 20mL? (1 point)
To detect the DNA band in your gel, you need to add ethidium bromide (EtBr) from a concentrated stock solution (40 ug/ml) to get a final concentration of 5 ug/ml in your agarose solution. Unfortunately, you made your agarose solution (0.8% agarose and 50 ml volume) before adding the EtBr. Thus, when you add he appropriate volume of concentrated EtBr, you will increase the fila volume of agarose solution. a. What volume of the concentrated EtBr must you add to...