Homework Answers
1). Isolate desiredmRNA
2. Make labelledcDNA
3. Hybridize cDNAs in microarray
4. Wash away unbound cDNAs
5.Place in scanner
6. Interpret fluorescnt spots
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Steps of microarray analysis Place the steps of DNA microarray analysis in the correct order. Hybridize...
Arrange the steps in creating and using a DNA microarray in order from beginning to end. Start wi...
Arrange the steps in creating and using a DNA microarray in order from beginning to end. Start with the production of the chip. A silicon chip is prepared with synthetic single-stranded DNA with a known sequence that corresponds to a partial sequence of a specific gene Complementary DNA bind to the microarray. mRNA is extracted from cells for which gene expression is being studied Unbound cDNA is washed away and the remaining DNA is examined under UV light CDNA is...
Tissue-specific microarray analysis facilitates the comparison of gene expression in different tissues. In order to prepare...
Tissue-specific microarray analysis facilitates the comparison
of gene expression in different tissues. In order to prepare a
sample for microarray analysis, messenger RNA (mRNA) is extracted
from a particular tissue, reverse transcribed to generate
complementary DNA (cDNA), and labeled with a fluorescent probe.
The microarray below shows comparison of gene expression in
stomach and intestinal tissues. The cDNA from stomach tissue was
labeled with red fluorescent probes, whereas the cDNA from the
intestinal tissue was labeled with green fluorescent probes....
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are lacking a OH at the...
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are
lacking a OH at the
a.
2' carbon
b.
5' carbon
c.
3' carbon
please answer as many as you can!! I am low on questions and
could use the help!
Mother || Child II Male 1 Male 2 Results from a paternity test using DNA fingerprinting is shown. DNA was isolated from a mother, her child and 2 potential fathers. Primers designed to amplify different satellite DNA regions...
please help table 1 , Question 1 and 10 procedure is done which is the color...
please help
table 1 , Question 1 and 10
procedure is done which is the color result
taly Color in results from the slide microarray or attach a photo Label each gene appropriately O O o ооо Figure 1. Color Intensity chart Expression Ratios 16 1/4 1/8 1/16 Table 1 Gene 1 Gene 2 Gene 3 Gene 4 Gene 5 Gene 6 Expression Ratio Decimal Value Log2 Value Paper Microarray Exercise 1. Describe which genes were induced or repressed in...
(blocked) d. PCR amplification of DNA fragments, cluster growth Below are some of the steps that...
(blocked) d. PCR amplification of DNA fragments, cluster
growth
Below are some of the steps that occur in a Nextgen genomic sequencing reaction. Fill in the missing steps. Steps MUST be in the proper order. a. Isolate and fragment genomic DNA from an organism Ev [ Select ] Add primer/ fluorescent chain terminating nucleotides Bind to chip Assemble contig Remove chain terminator Read fluorescence Ligate linkers C e. (Select ] f. Wash away excess, non-bound chain terminating nucleotides [Select] h....
Place the following steps of DNA replication in order from the beginning to the end of...
Place the following steps of DNA replication in order from the beginning to the end of the process. Your answer will be a series of numbers (1-5) in what you think is the correct order. 1. Joining of Okazaki fragments by DNA ligase 2. Priming of DNA synthesis with RNA primer 3. Opening and stabilizing the DNA strands 4.removal of primers by DNA polymerase lexonuclease 5. elongation of RNA primers by DNA polymerase III
Place the steps of bacterial transformation in the correct order? A) One strand of the donor...
Place the steps of bacterial transformation in the correct order? A) One strand of the donor cell DNA is degraded B) Transformed DNA pairs with homologous region on the recipient cell chromosome C)Donor cell DNA binds to a receptor site on teh recipient cel D) Donor cell, lyses released piees of its chromosome into the environment E)Transformed DNA recombines with the recipient cell chromosome.
Place the following steps of TRANSLATION in the correct order for EUKARYOTES. The ribosome reaches a...
Place the following steps of TRANSLATION in the correct order for EUKARYOTES. The ribosome reaches a stop codon. A release factor binds and causes the release of the new polypeptide, along with the mRNA. The ribosome dissociates. v Acharged tRNA with a matching anticodon binds the mRNA codon in the A site. ✓ The ribosome moves exactly 3 nucleotides toward the 3* end of the mRNA. The small ribosomal subunit uses rRNA to bind to the Kozak sequence, which places...
Exercise 2 Separation of a Mixture Based on Acid-Base Properties One purpose of this exercise is...
Exercise 2 Separation of a Mixture Based on Acid-Base Properties One purpose of this exercise is to learn how to use a separatory funnel to extract a single component away from other compounds in solution. To do so, we will apply the principles of solubility and acid-base behavior you’re seeing in class. One of the compounds is neutral in the acid-base sense. It has no ability to either donate or accept a proton from an aqueous solution, and will remain...
Arrange the steps in creating and using a DNA microarray in order from beginning to end. Start with the production of the chip. A silicon chip is prepared with synthetic single-stranded DNA with a known sequence that corresponds to a partial sequence of a specific gene Complementary DNA bind to the microarray. mRNA is extracted from cells for which gene expression is being studied Unbound cDNA is washed away and the remaining DNA is examined under UV light CDNA is...
Tissue-specific microarray analysis facilitates the comparison
of gene expression in different tissues. In order to prepare a
sample for microarray analysis, messenger RNA (mRNA) is extracted
from a particular tissue, reverse transcribed to generate
complementary DNA (cDNA), and labeled with a fluorescent probe.
The microarray below shows comparison of gene expression in
stomach and intestinal tissues. The cDNA from stomach tissue was
labeled with red fluorescent probes, whereas the cDNA from the
intestinal tissue was labeled with green fluorescent probes....
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are
lacking a OH at the
a.
2' carbon
b.
5' carbon
c.
3' carbon
please answer as many as you can!! I am low on questions and
could use the help!
Mother || Child II Male 1 Male 2 Results from a paternity test using DNA fingerprinting is shown. DNA was isolated from a mother, her child and 2 potential fathers. Primers designed to amplify different satellite DNA regions...
please help
table 1 , Question 1 and 10
procedure is done which is the color result
taly Color in results from the slide microarray or attach a photo Label each gene appropriately O O o ооо Figure 1. Color Intensity chart Expression Ratios 16 1/4 1/8 1/16 Table 1 Gene 1 Gene 2 Gene 3 Gene 4 Gene 5 Gene 6 Expression Ratio Decimal Value Log2 Value Paper Microarray Exercise 1. Describe which genes were induced or repressed in...
(blocked) d. PCR amplification of DNA fragments, cluster
growth
Below are some of the steps that occur in a Nextgen genomic sequencing reaction. Fill in the missing steps. Steps MUST be in the proper order. a. Isolate and fragment genomic DNA from an organism Ev [ Select ] Add primer/ fluorescent chain terminating nucleotides Bind to chip Assemble contig Remove chain terminator Read fluorescence Ligate linkers C e. (Select ] f. Wash away excess, non-bound chain terminating nucleotides [Select] h....
Place the following steps of DNA replication in order from the beginning to the end of the process. Your answer will be a series of numbers (1-5) in what you think is the correct order. 1. Joining of Okazaki fragments by DNA ligase 2. Priming of DNA synthesis with RNA primer 3. Opening and stabilizing the DNA strands 4.removal of primers by DNA polymerase lexonuclease 5. elongation of RNA primers by DNA polymerase III
Place the following steps of TRANSLATION in the correct order for EUKARYOTES. The ribosome reaches a stop codon. A release factor binds and causes the release of the new polypeptide, along with the mRNA. The ribosome dissociates. v Acharged tRNA with a matching anticodon binds the mRNA codon in the A site. ✓ The ribosome moves exactly 3 nucleotides toward the 3* end of the mRNA. The small ribosomal subunit uses rRNA to bind to the Kozak sequence, which places...
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