Question

1. What is the purpose of the promoter reg E. coli and in eukaryotes. (2.5 points each, total of 5 points) e or the promoter region? Draw and label a typical prom ical promoter in 2. Transcription and translation share the same overall steps. Name and briefly describe what happens during each step. You do not need to specific transcription factors or elongation factors. (10 points)

Answer 1

real celle Purpose of promate initiated Promato of expression "ponent of Promater Region - A prometer is a region of DNA where transcription of a gene ated Promotors are a vital component of es because they control the binding of Rh polymerase to DNA. RNA polymerase trims A to mRNA which is ultimately translated functional protein. RNA polymerase sene, and , Aromater Rogilon - care Promoter + Proximal Brometer - Distal Promoter a Padmodata Antisense Strand 27 Strand ing is very different in bacteria compared to Promceter binding is very different eukaryotes. In bacteria the Care RNA polymerase requires an associated sigma faction for promoter recognition and binding. On the other bond in Eukaryotes is much more complex. Erky Eukaryotes require a minimum of seven transcription factors in corder for RNA baelymerasett to bind to a prometer. . Fromscripth (STOP STOP " Transcription RNA Polymeran site! Ceeding Site betob I (TSS) : sequence i - I start Cadon steep Codon - Enfancer Prometer activaters Trandorition t o facteu thenter Eukaryotic Transeription Grene