If restriction is cleavage of incoming DNA in a bacterium, what is modification. Is it true...
If restriction is cleavage of incoming DNA in a bacterium, what is modification.
Is it true that Type II restriction endonucleases catalyze DNA hydrolysis within or near the enzyme recognition sequence and always at the same position?
Homework Answers
Restriction means cleavage or cutting of the DNA sequence at the desired site whereas modification means functional relevant changes in genome without altering the DNA nucleotide sequence for example, histone acetylation , DNA methylation .this modification alters how the gene is expressed in an organism, without having to change its DNA sequence. These changes are heritable and are called as epigenetics.
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If restriction is cleavage of incoming DNA in a bacterium, what
is modification.
Is it true...
RESTRICTION DIGEST ANALYSIS QUESTIONS(true or yes = A: false or no = b) 1. Larger DNA...
RESTRICTION DIGEST ANALYSIS QUESTIONS(true or yes = A: false or no = b) 1. Larger DNA fragments appear near the bottom of the gel. 2. Larger DNA fragments move more rapidly through the gel. D ONA that has many restriction sites for a certain endonuclease will be cut into more fragmets than DNA with fewer restriction sites. 4. Cutting DNA with many different endonucleases will result in more DNA fragments. 5. Restriction enzymes all recognize the same base sequence when...
Identify two restriction endonucleases that could be used to make sticky ends near the 5’ end of this DNA sequence (uppe...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
The first three bases of the 6-base recognition cleavage site of the restriction enzyme, HindIII are...
The first three bases of the 6-base recognition cleavage site of the restriction enzyme, HindIII are AAG. What is the complete sequence of this 6 bp site? Provide an explanation for your answer.
what is the purpose, claim,hypothesis and evidence for restriction endonucleases lab Biomolecular Techniques EXPERIMENT 2: RESTRICTION...
what is the purpose, claim,hypothesis and evidence for
restriction endonucleases lab
Biomolecular Techniques EXPERIMENT 2: RESTRICTION ENDONUCLEASES Data Tables Purpose (What question is this experiment designed to answer?): Does the fragment size affect the enzyme? Hypothesis (Based on what you've learned in the pre-lab materials, write and If/Then statement regarding the outcome of this experiment.) Table 1: Enzyme Analysis Cuts Between Number of Fragments Fragment Size (in order) 22,9,4 17,11,7 3 Enzyme 1 Enzyme 2 Enzyme 3 4 5,3,15,12 ОО...
2. 3. Give the recognition sequence for the restriction enzyme Eael in a dsDNA sequence, indicate...
2. 3. Give the recognition sequence for the restriction enzyme Eael in a dsDNA sequence, indicate the cleavage sites. (2) With reference to your PCR amplicon, detail the terminal DNA sequences at the two ends of the fragment that will be generated after digestion with Eael (indicate these in the two blocks below). What type of ends are generated by this enzyme (blunt/sticky, 5' or 3' overhang)? PCR amplicon بیا بیا
You've identified and purified a novel closed circular viral DNA, and want to treat it with a typ...
You've identified and purified a novel closed circular viral DNA, and want to treat it with a type II restriction enzyme used in BCH370. This DNA has a single restriction site contained within the following sequence: 5' GGGTTATGAATTCTACA 3' 3, CCCAATACTTAAGATGT 5, a) Indicate the probable region identified by the restriction enzyme, and explain how you can identify the recognition sequence based on certain criteria. (2 marks) b) You then attempt to identify whether or not the viral vector contains...
Pstl is a restriction enzyme produced by the bacterium Providencia stuartii. It recognizes the six base...
Pstl is a restriction enzyme produced by the bacterium Providencia stuartii. It recognizes the six base pair sequence 5-CTGCAG-3. Why is the chromosome of P. stuartii not degraded by Psti? CAR P. stuarti DNA is unmethylated within the recognition sequence for Pstl, and is not degraded by Pstl, which only digests methylated DNA B. It is not understood why the P. stuartii genome is not degraded by Psti. C. P. stuarti DNA is methylated within the recognition sequence for Pstl,...
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the...
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3' 3' CCGG 5 5' AAGCTT 3 3' TTCGAA 5 5' RGGWCCY 3 3' YCCWGGR 5 Cleavage 5G AATTC 3' 3' CTTAA G 5'...
Need Part C answered only. The table shows where different restriction endonucleases (restriction enzymes) cleave DNA....
Need Part C answered only.
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3' 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3 3' CCGG 5 5' AAGCTT 3' 3' TTCGAA 5 5'RGGWCCY 3 3' YCCWGGR 5 Cleavage 5'G AATTC 3'...
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this...
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this plasmid into two fragments. A. True B. False 2.In general, restriction enzymes that recognize four nucleotides have higher probability to produce more DNA fragments than those enzymes that recognize six nucleotides. A. true B. false 3. Which of the following sequences are palindromes? A. 5' TGGCCA 3' B. 5' GAAAAG 3' C. 5' CGATGG 3' D. 5' GACGAC 3' 4. Below are the possible...
RESTRICTION DIGEST ANALYSIS QUESTIONS(true or yes = A: false or no = b) 1. Larger DNA fragments appear near the bottom of the gel. 2. Larger DNA fragments move more rapidly through the gel. D ONA that has many restriction sites for a certain endonuclease will be cut into more fragmets than DNA with fewer restriction sites. 4. Cutting DNA with many different endonucleases will result in more DNA fragments. 5. Restriction enzymes all recognize the same base sequence when...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
what is the purpose, claim,hypothesis and evidence for
restriction endonucleases lab
Biomolecular Techniques EXPERIMENT 2: RESTRICTION ENDONUCLEASES Data Tables Purpose (What question is this experiment designed to answer?): Does the fragment size affect the enzyme? Hypothesis (Based on what you've learned in the pre-lab materials, write and If/Then statement regarding the outcome of this experiment.) Table 1: Enzyme Analysis Cuts Between Number of Fragments Fragment Size (in order) 22,9,4 17,11,7 3 Enzyme 1 Enzyme 2 Enzyme 3 4 5,3,15,12 ОО...
2. 3. Give the recognition sequence for the restriction enzyme Eael in a dsDNA sequence, indicate the cleavage sites. (2) With reference to your PCR amplicon, detail the terminal DNA sequences at the two ends of the fragment that will be generated after digestion with Eael (indicate these in the two blocks below). What type of ends are generated by this enzyme (blunt/sticky, 5' or 3' overhang)? PCR amplicon بیا بیا
You've identified and purified a novel closed circular viral DNA, and want to treat it with a type II restriction enzyme used in BCH370. This DNA has a single restriction site contained within the following sequence: 5' GGGTTATGAATTCTACA 3' 3, CCCAATACTTAAGATGT 5, a) Indicate the probable region identified by the restriction enzyme, and explain how you can identify the recognition sequence based on certain criteria. (2 marks) b) You then attempt to identify whether or not the viral vector contains...
Pstl is a restriction enzyme produced by the bacterium Providencia stuartii. It recognizes the six base pair sequence 5-CTGCAG-3. Why is the chromosome of P. stuartii not degraded by Psti? CAR P. stuarti DNA is unmethylated within the recognition sequence for Pstl, and is not degraded by Pstl, which only digests methylated DNA B. It is not understood why the P. stuartii genome is not degraded by Psti. C. P. stuarti DNA is methylated within the recognition sequence for Pstl,...
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3' 3' CCGG 5 5' AAGCTT 3 3' TTCGAA 5 5' RGGWCCY 3 3' YCCWGGR 5 Cleavage 5G AATTC 3' 3' CTTAA G 5'...
Need Part C answered only.
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3' 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3 3' CCGG 5 5' AAGCTT 3' 3' TTCGAA 5 5'RGGWCCY 3 3' YCCWGGR 5 Cleavage 5'G AATTC 3'...
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