Homework Answers
The antisense reagent can be designed to pair specifically with RNA, not DNA. The morpholino class of antisense reagents is one such agent, which binds in sequence-specific fashion to RNA. Therefore, translation is preferentially inhibited, with minimal effect on transcription.
Morpholino class of antisense reagents are usually used to knock down genes. In this technique, antisense single stranded DNA or RNA are prepared which will bind to the specific RNA and degrade it. This will block the gene expression or translation.
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sequence-non-specific sequence-specific translation The antisense reagent can be designed to pair specifically with , not The...
1 Which of the following represents one of the mechanisms by which antisense agents exert their...
1 Which of the following represents one of the mechanisms by which antisense agents exert their effect? a. A short DNA sequence binds to specific regions of complementary mRNA, inducing a nuclease that promotes translation to the corresponding protein b. Antisense oligonucleotides prevent the natural silencing of targeted mRNA sequences C. A short DNA sequence binds to specific regions of complementary mRNA, inducing a nuclease that cleaves the mRNA d. Antisense oligonucleotides inhibit all types of gene splicing 2 The...
25. What is a riboswitch? A. a small molecule that regulates the translation of specific mRNAs...
25. What is a riboswitch? A. a small molecule that regulates the translation of specific mRNAs B. a gene regulatory protein that turns on the expression of ribosomal proteins C. an mRNA that can regulate its own transcription and translation D. a ribosome that participates in attenuation E. when the antisense RNA binds to the transcript 26. In the Ames Test, the appearance of his+ revertants in the presence of a non-mutagenic control compound indicates that _______. A. liver extract...
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A....
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A. Only ribonucleoside triphosphates contain the sugar ribose. B. Ribonucleoside triphosphates have low potential energy, making the polymerization reaction endergonic. C. Ribonucleoside triphosphates have high potential energy, making the polymerization reaction exergonic. D. Ribonucleoside monophosphates cannot form complementary base pairs with the DNA template. E. Ribonucleoside triphosphates are not used, rather all use deoxyriboside triphosphates. 14. How is a mutation in a bacterial cell that...
need to know correct answers 26. B. Which of the following enzymes is associated with the...
need to know correct answers
26. B. Which of the following enzymes is associated with the repression of transcription? methyl transferase DNA polymerasex acetyl transferase histone kinase none of the above Section 2 - True/False (2 points each) 27. TFIIB regulates tissue or cell type-specific transcription 28._1 Eukaryotes have three different RNA polymerases that each can transcribe non-protein coding genes. 29. T The P site of the ribosome refers to peptidyl. be For T heoph UND 30._F_The input genetic material...
5. Which of the followin g is After the DNA unwinds only one strand acts as...
5. Which of the followin g is After the DNA unwinds only one strand acts as a compliance The two strands only act as a plate when paired In prokaryotes the binding of RNAP the two strands After the DNA unwind hath DNA strands at templates occurs randomly on either of of RNA polymerase to unwound DNA Occurs 14. What is an anticodon? The three RNA behaal with nifie umino acid. The three RNA bases that air with a c...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
EXPERIMENT 9 CHEMICAL REACTIONS: STOICHIOMETRY LABORATORY REPORT Your Name TA's Name You must rea...
EXPERIMENT 9 CHEMICAL REACTIONS: STOICHIOMETRY LABORATORY REPORT Your Name TA's Name You must read pages 69 and 70 to complete the following calculations. Lab Section 1411. Date Trial #1 Trial #2 Trial #3 vol . 0.100 M K2CO, solution -1200 mL 12.00 mL mmole K2CrO vol. 0.100 M Pb(NO,), solution 10.00ml mmole Pb(NO) 12.00m 14.00 mL 20 g mass product+paper 0.579 g0.654 g mass dry filter paper 0266 mass PbCrO, collected precip. 9 mmole Pbcro, CALCULATIONS AND QUESTIONS (USE THE...
How can we assess whether a project is a success or a failure? This case presents...
How can we assess whether a project is a success or a
failure?
This case presents two phases of a large business transformation project involving the implementation of an ERP system with the aim of creating an integrated company. The case illustrates some of the challenges associated with integration. It also presents the obstacles facing companies that undertake projects involving large information technology projects. Bombardier and Its Environment Joseph-Armand Bombardier was 15 years old when he built his first snowmobile...
1 Which of the following represents one of the mechanisms by which antisense agents exert their effect? a. A short DNA sequence binds to specific regions of complementary mRNA, inducing a nuclease that promotes translation to the corresponding protein b. Antisense oligonucleotides prevent the natural silencing of targeted mRNA sequences C. A short DNA sequence binds to specific regions of complementary mRNA, inducing a nuclease that cleaves the mRNA d. Antisense oligonucleotides inhibit all types of gene splicing 2 The...
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A. Only ribonucleoside triphosphates contain the sugar ribose. B. Ribonucleoside triphosphates have low potential energy, making the polymerization reaction endergonic. C. Ribonucleoside triphosphates have high potential energy, making the polymerization reaction exergonic. D. Ribonucleoside monophosphates cannot form complementary base pairs with the DNA template. E. Ribonucleoside triphosphates are not used, rather all use deoxyriboside triphosphates. 14. How is a mutation in a bacterial cell that...
need to know correct answers
26. B. Which of the following enzymes is associated with the repression of transcription? methyl transferase DNA polymerasex acetyl transferase histone kinase none of the above Section 2 - True/False (2 points each) 27. TFIIB regulates tissue or cell type-specific transcription 28._1 Eukaryotes have three different RNA polymerases that each can transcribe non-protein coding genes. 29. T The P site of the ribosome refers to peptidyl. be For T heoph UND 30._F_The input genetic material...
5. Which of the followin g is After the DNA unwinds only one strand acts as a compliance The two strands only act as a plate when paired In prokaryotes the binding of RNAP the two strands After the DNA unwind hath DNA strands at templates occurs randomly on either of of RNA polymerase to unwound DNA Occurs 14. What is an anticodon? The three RNA behaal with nifie umino acid. The three RNA bases that air with a c...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
EXPERIMENT 9 CHEMICAL REACTIONS: STOICHIOMETRY LABORATORY REPORT Your Name TA's Name You must read pages 69 and 70 to complete the following calculations. Lab Section 1411. Date Trial #1 Trial #2 Trial #3 vol . 0.100 M K2CO, solution -1200 mL 12.00 mL mmole K2CrO vol. 0.100 M Pb(NO,), solution 10.00ml mmole Pb(NO) 12.00m 14.00 mL 20 g mass product+paper 0.579 g0.654 g mass dry filter paper 0266 mass PbCrO, collected precip. 9 mmole Pbcro, CALCULATIONS AND QUESTIONS (USE THE...
How can we assess whether a project is a success or a
failure?
This case presents two phases of a large business transformation project involving the implementation of an ERP system with the aim of creating an integrated company. The case illustrates some of the challenges associated with integration. It also presents the obstacles facing companies that undertake projects involving large information technology projects. Bombardier and Its Environment Joseph-Armand Bombardier was 15 years old when he built his first snowmobile...
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