Question

Match the following phenotypes with the mutation that would be the most likely to be its cause. The mutant proteins are listed below, followed by the protein domain that is mutated in each case, followed by the amino acid change that is a result of the mutation Try to answer each question by using the following reasoning: if you observe a phenotype, then that could be due to a mutation in protein in which the domain has had an amino acid change fromto Example After a few rounds of cell replication, yeast cells die and their chromosomes are much shorter than those found in wild-type yeast. A: telomerase (the most likely protein mutated to give you this phenotype) 1. Mutant human cells die, and you observe that the DNA in the cells is not condensed prior to cell division 2· E. coli DNA replication does not take place, and you observe no single-stranded DNA in the mutant cells 3. In mutant E coli, you observe Pol III core proteins floating freely in the cell removed from the holoenzyme complex 4.- _ In mutant E. coli, you see very low processivity and you observe high disassociation of Pol III and the DNA strand

Answer 1

Answers:

1. N: Histone H1 : Arg to Ala

2.Pol III : Finger domain: : Asp to Ala

3.S) Sliding clamp : PolII binding domain: Ala to Gln

4.D) SBP : DNA binding protein:Arg to LyS