The concentration of NaCl in MSA is 7.5%. a) What is the prupose of this high NaCl concentration? Give a detailed explanation. b) What is the molarity of NaCl in this medium? Show your calculation. (M.W. of NaCl 58.44)
Answer:
Mannitol salt agar contains a high concentration NaCl (7.5%). This makes it selective media for Gram-positive bacteria (Staphylococcus and Micrococcaceae).
It inhibits the growth of other bacteria.
Molarity of NaCl
7.5g per 100mL
75g in 1000mL
Number of moles= weight in g/Mol wt = 7.5/58.44 =1.2
Molarity= number of moles/volume in litre = 1.2/1L = 1.2 M
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