Question

Protein Binding

Three membrane receptor proteins bind tightly to a hormone. The free hormone concentrations and their corresponding fractions of hormone binding, Y, are given in the table.

[Hormone], nMProtein 1 (Y)Protein 2 (Y)Protein 3 (Y)
0.200.0480.290.17
0.500.110.500.33
1.00.200.670.50
4.00.500.890.80
100.710.950.91
200.830.970.95
500.930.990.98

Select the Kd for hormone binding by protein 1.

0.93 nM

50 nM

0.20 nM

4.0 nM

Select the protein that binds most tightly to the hormone.

protein 1

protein 3

protein 2


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