Scientists have genetically engineered a new DNA polymerase named Phusion polymerase. What is Phusion polymerase and...
Scientists have genetically engineered a new DNA polymerase named Phusion polymerase. What is Phusion polymerase and what properties does it have in comparison to Taq polymerase? (Hint: an excellent answer will make reference to parts of the E. coli replisome). (4 marks)
Homework Answers
when pyrococcus like enzyme is fused with the processivity enhancing domain results in increase in fiedility and speed .phusion polymerase is an ideal choice beacuse it can be used for difficult amplicons.its error rate is also lower than the taq ploymerase. it possess 5'to 3'polymerase activity and 3'to 5'exonuclease activity and also thermostabe.
Add Answer to:
Scientists have genetically engineered a new DNA
polymerase named Phusion polymerase. What is Phusion
polymerase and...
A company hires independent university scientists to determine whether its new, genetically engineered soybean poses any...
A company hires independent university scientists to determine whether its new, genetically engineered soybean poses any threat to the environmen Are there sources of bias in this situation? Choose the correct answer below O A. There do not appear to be any sources of bias B. There does appear to be sources of bias
What are genetically modified organisms (GMOs)? Plants have been genetically engineered to contain added nutrients for...
What are genetically modified organisms (GMOs)? Plants have been genetically engineered to contain added nutrients for better human health or pesticide resistance genes to decrease the use of pesticides. Some scientists fear that GMO crops containing added foreign genes may lead to the generation of 'super weeds'. Do you think the pros of GMOs outweigh the cons?
Compare the activities of E. coli DNA polymerase III and RNA polymerase by describing the following...
Compare the activities of E. coli DNA polymerase III and RNA polymerase by describing the following properties of each: DNAP III (Replication) RNAP (Transcription) Substrate(s) i.e. what does the enzyme link together Name for the site of enzyme binding Is a primer required? What serves as template(s)? End product Direction of polymerization
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help...
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the...
Scientists at the University of Iowa are working on a live, genetically COVID-19 vaccine. They have...
Scientists at the University
of Iowa are working on a live, genetically COVID-19 vaccine. They
have inserted the genes that encode COVID-19 envelop protein a
virus (parainfluenza). In the diagram, 1) viral vector vaccine.
Parainfluenza virus causes mild influenza-like
symptoms.
Diagram or explain the steps that were taken to get the
COVID RNA into the parainfluenza host. Remember to make the
parainfluenza virus non-pathogenic.
How can Pfizer scale up production of the genetically
engineered parainfluenza virus to produce quantities enough...
A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the...
A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the structural genes with the reporter gene green fluorescent protein (GFP). The repressor allele cannot bind lactose, and the OC allele cannot be bound by the repressor. Given the following genotypes and growth media that does not contain glucose, indicate in the table below whether you expect to see b-galactosidase activity and glowing green colonies of E. coli by selecting Yes or No. growth media...
46. What enzyme is used to make cDNA a. Ribozyme b. Reverse transcriptase C. Taq polymerase...
46. What enzyme is used to make cDNA a. Ribozyme b. Reverse transcriptase C. Taq polymerase d. Restriction endonuclease 47. Which of the following requires contact between a virus and a recipient bacterium for transfer of DNA? a. Crossing over b. Mutation c. Transduction d. Conjugation e. Transformation 48. In Recombinant DNA technology a vector is used to inserts the DNA into a host cell True/ False 49. If a foreign gene inserted into a plasmid inactivates the beta-galactosidase gene,...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
1. If DNA polymerase III was going to attach a new nucleotide onto the DNA molecule shown to the right, where would it attach it? 2. Why are the ends labeled A and C different? a. The diagram is...
1. If DNA polymerase III was going to attach a new nucleotide onto the DNA molecule shown to the right, where would it attach it? 2. Why are the ends labeled A and C different? a. The diagram is drawn incorrectly, they should be the same One is o pure onsa pyrimidine. c. One is a purine, one is a pyrimidine d. Double-stranded DNA is antiparallel. e. This is after DNA replication -before DNA replication, they look the same. 3....
If you start with 47 DNA segments, how many total copies of DNA will you have...
If you start with 47 DNA segments, how many total copies of DNA will you have after 12 cycles of PCR? Also what does attenuated mean in regards to the K12 strain of E. coli being used in lab? Is the answer “we have removed the gene that allows the bacteria to replicate”? Thank you!!
A company hires independent university scientists to determine whether its new, genetically engineered soybean poses any threat to the environmen Are there sources of bias in this situation? Choose the correct answer below O A. There do not appear to be any sources of bias B. There does appear to be sources of bias
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the...
Scientists at the University
of Iowa are working on a live, genetically COVID-19 vaccine. They
have inserted the genes that encode COVID-19 envelop protein a
virus (parainfluenza). In the diagram, 1) viral vector vaccine.
Parainfluenza virus causes mild influenza-like
symptoms.
Diagram or explain the steps that were taken to get the
COVID RNA into the parainfluenza host. Remember to make the
parainfluenza virus non-pathogenic.
How can Pfizer scale up production of the genetically
engineered parainfluenza virus to produce quantities enough...
A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the structural genes with the reporter gene green fluorescent protein (GFP). The repressor allele cannot bind lactose, and the OC allele cannot be bound by the repressor. Given the following genotypes and growth media that does not contain glucose, indicate in the table below whether you expect to see b-galactosidase activity and glowing green colonies of E. coli by selecting Yes or No. growth media...
46. What enzyme is used to make cDNA a. Ribozyme b. Reverse transcriptase C. Taq polymerase d. Restriction endonuclease 47. Which of the following requires contact between a virus and a recipient bacterium for transfer of DNA? a. Crossing over b. Mutation c. Transduction d. Conjugation e. Transformation 48. In Recombinant DNA technology a vector is used to inserts the DNA into a host cell True/ False 49. If a foreign gene inserted into a plasmid inactivates the beta-galactosidase gene,...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
1. If DNA polymerase III was going to attach a new nucleotide onto the DNA molecule shown to the right, where would it attach it? 2. Why are the ends labeled A and C different? a. The diagram is drawn incorrectly, they should be the same One is o pure onsa pyrimidine. c. One is a purine, one is a pyrimidine d. Double-stranded DNA is antiparallel. e. This is after DNA replication -before DNA replication, they look the same. 3....
Most questions answered within 3 hours.
-
Do not neglect the old for the new. The existing business must
not lose priority simply...
asked 1 hour ago -
Kylie is a single mom with two dependent children,
Tanner, age 7 and Olivia, age 11....
asked 3 hours ago -
Phosphorous + bromine = phosphorous tribromide. If 35.0 g of
bromine are reacted and 27.9 grams...
asked 4 hours ago -
Derive the long wavelength limit of the Planck energy density
distribution
asked 4 hours ago -
Calculate the pH of each of the following solutions.
0.50 M HBr
3.1×10−4 M KOH
4.2×10−5...
asked 8 hours ago -
For the year ended December 31, Depot Max’s cost of merchandise
sold was $85,600. Inventory at the...
asked 8 hours ago -
Week 10 - Professional Memo Assignment
Professional Memo Assignment
Your mission for this week, should you...
asked 8 hours ago -
Write a Python program that stores the data for each
player on the team, and it...
asked 8 hours ago -
In
the last 3 months, mike never knows when he is going to get his
allowance...
asked 8 hours ago -
Is Ca(OH)2 a Bronsted base, Lewis base, or both? Why?
asked 8 hours ago -
1A- Why don’t voters complain about U.S. tariffs on imported
sugar?
Because sugar is only a...
asked 8 hours ago -
Cash Payback Period
Primera Banco is evaluating two capital investment proposals for
a drive-up ATM kiosk,...
asked 8 hours ago