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3. (2) You are comparing the DNA from...
In a particular region of the genome of a certain bacterium, one DNA strand is transcribed...
In a particular region of the genome of a certain bacterium, one DNA strand is transcribed to give rise to an mRNA for protein A and the other DNA strand is transcribed to give rise to an mRNA for protein B. a) Would there be any problem in expressing the two genes? b) If a mutation occurred to affect the structure of protein A, what would you observe in the structure of protein B?
1) Using the bacterial DNA sequence that the instructor gave you:
1) Using the bacterial DNA sequence that the instructor gave you:a. Identify and underline the promoter region and the start codon.b. Identify the coding and template strandc. Transcribe the coding sequenced. Translate the mRNA sequence8) Which of the following mutational changes would you predict to be the most deleterious to gene function? Explain your answers.a. Insertion of a single nucleotide near the end of the coding sequence.b. Removal of a single nucleotide near the beginning of the coding sequence.c. Deletion...
Shown below is the anti-sense DNA sequence from a region of a gene that produces a...
Shown below is the anti-sense DNA sequence from a region of a gene that produces a specific protein. Mutations in this region of the gene cause a disease CTT TTA TAG TAG ATA CCA CAA AGG a. What is the mRNA strand that is transcribed from the DNA shown above? b. What is the amino acid sequence that would be translated from the mRNA strand you determined in part 1? c. If an individual has a G at position 15...
4. In future lectures we will describe a technique known as Northern blotting that can be...
4. In future lectures we will describe a technique known as Northern blotting that can be used to detect RNA transcribed from a particular gene. Briefly, in this method a specific RNA is detected using a short segment of cloned DNA as a probe. The DNA probe, which is radioactive, is complementary to the RNA that the researcher wishes to detect. After the radioactive probe DNA basepairs to the RNA, the RNA is visualized as a dark (radioactive) band on...
One strand of a section of DNA isolated from E. coli reads: 5’GTAGCCTACCCATAGG-3’
One strand of a section of DNA isolated from E. coli reads: 5’GTAGCCTACCCATAGG-3’ (a) Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will the sequence of the mRNA in this region be? (b) What is the amino acid sequence of the proteins. (c) Would the same proteins be made if the other strand of DNA served as template for transcription? Why or why not. (d) If the T underlined in the above sequence was to...
question 37 and 38 please 36. Which one do you think would be more harmful. A...
question 37 and 38 please
36. Which one do you think would be more harmful. A mutation that changed the nucleotide sequence of an mRNA molecule, or a mutation that changed the nucleotide sequence of a DNA molecule? 5 points 37. All body cells have the same DNA complement yet all body cells do NOT contain the same proteins. How is this possible? 5 points 38. Consider a bacterial gene that is approximately 2100 nucleotides long. 3 Points Approximately how...
can you guys please give me the correct answers and explain why? 9. Which of the...
can you guys please give me the correct answers and explain
why?
9. Which of the following statements is INCORRECT? A. Most human cells contain high levels of telomerase. B. Telomeres protect the ends of eukaryotic chromosomes. C. Telomerase is ribonucleoprotein D. Each round of replication shortens eukaryotic chromosomes. E. Telomerase is an RNA-dependent DNA polymerase. 10. DNA in front of the replication fork is positively supercoiled (over-wound). What is the source of energy required for this over-winding? Pollll Core...
This DNA sequence comes from part of a gene on a chromosome of a eukaryotic organism.
This DNA sequence comes from part of a gene on a chromosome of a eukaryotic organism. i) If the bottom strand of the sequence is the template strand and the top strand is the coding strand, circle the answer below that best represents the direction that RNA polymerase would move along the template DNA strand. Left to Right Right to Left Lagging to Leading A site to P site ii) Given the information in 6 i, write the sequence of the mRNA...
6. Using the answers to questions 2-5 and the below DNA sequence, predict the mRNA sequence,...
6. Using the answers to questions 2-5 and the below DNA sequence, predict the mRNA sequence, the tRNA anticodons, and the amino acid sequences (use the three letter code) that would result from it. (3 points) DNA +1 15'GICIA I G C A A CICATI I AA GG 3' 3" CA GATA C GTIGA GIA A A IICC 5 mRNA tRNA anticodons amino acids 7. You are interested in a gene that codes for a 20 amino acid-long protein. (1.5...
Review Questions BIOL 260: Chapters 8-10, 13, 19 1. Consider a mutation involving the deletion of...
Review Questions BIOL 260: Chapters 8-10, 13, 19 1. Consider a mutation involving the deletion of either 1, 2, or 3 nucleotides in the DNA of a bacterium. Which of these mutations (ie., deletion of 1, 2, or 3 nucleotides) would likely have the LEAST impact on the organism? Why? Include in your answer a comparison with the other two options to justify your reasoning. Think carefully about the impact each mutation would have on the ultimate protein coded for...
1) Using the bacterial DNA sequence that the instructor gave you:a. Identify and underline the promoter region and the start codon.b. Identify the coding and template strandc. Transcribe the coding sequenced. Translate the mRNA sequence8) Which of the following mutational changes would you predict to be the most deleterious to gene function? Explain your answers.a. Insertion of a single nucleotide near the end of the coding sequence.b. Removal of a single nucleotide near the beginning of the coding sequence.c. Deletion...
4. In future lectures we will describe a technique known as Northern blotting that can be used to detect RNA transcribed from a particular gene. Briefly, in this method a specific RNA is detected using a short segment of cloned DNA as a probe. The DNA probe, which is radioactive, is complementary to the RNA that the researcher wishes to detect. After the radioactive probe DNA basepairs to the RNA, the RNA is visualized as a dark (radioactive) band on...
question 37 and 38 please
36. Which one do you think would be more harmful. A mutation that changed the nucleotide sequence of an mRNA molecule, or a mutation that changed the nucleotide sequence of a DNA molecule? 5 points 37. All body cells have the same DNA complement yet all body cells do NOT contain the same proteins. How is this possible? 5 points 38. Consider a bacterial gene that is approximately 2100 nucleotides long. 3 Points Approximately how...
can you guys please give me the correct answers and explain
why?
9. Which of the following statements is INCORRECT? A. Most human cells contain high levels of telomerase. B. Telomeres protect the ends of eukaryotic chromosomes. C. Telomerase is ribonucleoprotein D. Each round of replication shortens eukaryotic chromosomes. E. Telomerase is an RNA-dependent DNA polymerase. 10. DNA in front of the replication fork is positively supercoiled (over-wound). What is the source of energy required for this over-winding? Pollll Core...
6. Using the answers to questions 2-5 and the below DNA sequence, predict the mRNA sequence, the tRNA anticodons, and the amino acid sequences (use the three letter code) that would result from it. (3 points) DNA +1 15'GICIA I G C A A CICATI I AA GG 3' 3" CA GATA C GTIGA GIA A A IICC 5 mRNA tRNA anticodons amino acids 7. You are interested in a gene that codes for a 20 amino acid-long protein. (1.5...
Review Questions BIOL 260: Chapters 8-10, 13, 19 1. Consider a mutation involving the deletion of either 1, 2, or 3 nucleotides in the DNA of a bacterium. Which of these mutations (ie., deletion of 1, 2, or 3 nucleotides) would likely have the LEAST impact on the organism? Why? Include in your answer a comparison with the other two options to justify your reasoning. Think carefully about the impact each mutation would have on the ultimate protein coded for...
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