A bacterial strain is auxotrophic for leucine and histidine. Which medium would allow bacterial colonies to...
A bacterial strain is auxotrophic for leucine and histidine. Which medium would allow bacterial colonies to grow?
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An undefined media used for the growth of bacteria because this media contain amino acids for growth of bacteria.
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A bacterial strain is auxotrophic for leucine and histidine.
Which medium would allow bacterial colonies to...
A streptomycin-sensitive Hfr strain, prototrophic for threonine (thr), leucine (leu), arginine (arg), and histidine (his), was...
A streptomycin-sensitive Hfr strain, prototrophic for threonine
(thr), leucine (leu), arginine (arg), and histidine (his), was
allowed to conjugate with a streptomycin-resistant F- strain whose
genotype was thr- leu- arg- his-. When plated on complete medium
containing streptomycin, a number of colonies arose. Cells from
five of these colonies were then tested on minimal medium
supplemented with added nutrients as indicated below. What were the
genotypes of the five colonies. PLEASE EXPLAIN.
MINIMAL MEDIUM PLUS COLONIES THR LEU THR HIS...
True/False 6. a)A tryptophan auxotrophic strain (trp¯) of bacteria will be able to grow on a...
True/False 6. a)A tryptophan auxotrophic strain (trp¯) of bacteria will be able to grow on a minimal medium agar that has been supplemented with the amino acid histidine. b). Hfr strains result from the insertion and recombination of the F factor plasmid into the bacterial chromosome. c). In generalized transduction, any gene or set of genes on the chromosome may be transferred from the donor bacterium to the recipient bacterium. d). The order of genes on a bacterial chromosome can...
A number of mutants were isolated in yeast that are auxotrophic for leucine. Different crosses were...
A number of mutants were isolated in yeast that are auxotrophic for leucine. Different crosses were performed between the different Leu- strains. In which of the following crosses would a geneticist expect the resulting diploid to grow on medium lacking leucine? A. A diploid containing two mutant alleles of the same gene that have the same nucleotide sequence. B. A diploid containing two mutant alleles of the same gene that have different nucleotide sequences. C. A diploid with a point...
A cross was performed between a yeast strain that requires histidine and methionine for growth (His-Met-)...
A cross was performed between a yeast strain that requires histidine and methionine for growth (His-Met-) and another yeast strain, which is His+Met+. One hundred asci were dissected, and colonies were grown from the four spores in each ascus. Cells from these colonies were tested for their ability to grow on petri plates containing either minimal medium(min), min+histidine (his), min+methionine (met),or min+his+met. The number of tetrads with four spores that can grow in different type of media is shown in...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and a live bacterial cell is: a. conjugation b. transduction c. transversion d. transformation 2. An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+...
6. A bacterial geneticist mixes 109 cells of E. coli strain A which requires lysine and...
6. A bacterial geneticist mixes 109 cells of E. coli strain A which requires lysine and is ampicillin sensitive with 109 cells of strain B which requires phenylalanine and tyrosine and is ampicillin resistant. In order to detect whether genetic recombination had occurred between the two strains it would be best to grow the mixture of cells in A) minimal glucose medium plus ampicillin B) minimal glucose medium with no supplements C) minimal glucose medium plus lysine and phenylalanine D)...
strain (thrleut his pro pan bio). At each gene, + . (5 points) One triply auxotrophic...
strain (thrleut his pro pan bio). At each gene, + . (5 points) One triply auxotrophic E.coli strain (thr leur his pro pant bio*) is transforme ONA from a second triply auxotro ndicates prototrophy. - auxotrophy. After transformation, bacteria are plated on complete the master plate) and then replica plated onto minimal medium or minimal media with add nutrients as indicted in the figure below (recall that replica plating means that the positio colonies is the same in all plates)....
This graph contains the titration curve of which of these amino acids: leucine, histidine, or lysine?...
This graph contains the titration curve of which of these amino
acids: leucine, histidine, or lysine? Explain.
14.0r 12.0 10.0 8.0 6.0 4.0 2.0 a 60 Volume of base added
An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain...
An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+ after 30 minutes a+ b+e+ after 40 minutes a+b+d+e+ after 50 minutes a+b+c+d+e+ after 60 minutes a+b+c+d+e+f+ Which represents the correct order of genes on this bacterial chromosomes? efabcd eabdcf...
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling...
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling time of about 30 minutes. If you start with 10,000 cells per mL of culture, and let them grow exponentially 2 hours in a flask, what is the final density of the culture? Show and explain your calculations. b) If you plated a100μL of 1:1000 dilution that culture (after it had grown in a flask for 2 hrs) how many colonies would you expect...
A streptomycin-sensitive Hfr strain, prototrophic for threonine
(thr), leucine (leu), arginine (arg), and histidine (his), was
allowed to conjugate with a streptomycin-resistant F- strain whose
genotype was thr- leu- arg- his-. When plated on complete medium
containing streptomycin, a number of colonies arose. Cells from
five of these colonies were then tested on minimal medium
supplemented with added nutrients as indicated below. What were the
genotypes of the five colonies. PLEASE EXPLAIN.
MINIMAL MEDIUM PLUS COLONIES THR LEU THR HIS...
A cross was performed between a yeast strain that requires histidine and methionine for growth (His-Met-) and another yeast strain, which is His+Met+. One hundred asci were dissected, and colonies were grown from the four spores in each ascus. Cells from these colonies were tested for their ability to grow on petri plates containing either minimal medium(min), min+histidine (his), min+methionine (met),or min+his+met. The number of tetrads with four spores that can grow in different type of media is shown in...
strain (thrleut his pro pan bio). At each gene, + . (5 points) One triply auxotrophic E.coli strain (thr leur his pro pant bio*) is transforme ONA from a second triply auxotro ndicates prototrophy. - auxotrophy. After transformation, bacteria are plated on complete the master plate) and then replica plated onto minimal medium or minimal media with add nutrients as indicted in the figure below (recall that replica plating means that the positio colonies is the same in all plates)....
This graph contains the titration curve of which of these amino
acids: leucine, histidine, or lysine? Explain.
14.0r 12.0 10.0 8.0 6.0 4.0 2.0 a 60 Volume of base added
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