Question

3. The following is a DNA coding strand sequence encoding the C-terminus of a protein: 5...A TCTTG GTTGACAACAGTT G A...3
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The C is located between the two Gs
A T C T T G "C" G
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Answer:

The given DNA coding sequence encoding the C-terminus of the protein is:

5'-ATCTTGGTTGACAACAGTTGA--3'

If a C nucleotide is inserted at the location indicated by the arrow, describe the effect of the mutation on the protein

The new sequence after the insertion of the C is:

5'-ATCTTGCGTTGACAACAGTTGA--3'

Effect of this mutation on protein

The mRNA sequence correspoding to this coding strand will be:

5'- AUCUUGCGUUGACAACAGUUGA - 3'

The sequence of protein that will be translated from the mRNA sequence is:

Isoleucine-Leucine-Arginine-Stop codon

The mutation will cause the translation to terminate after Arginine due to the presence of stop codon.

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