Homework Answers
Question- which of the following technique could be used to accurately and precisely quantify gene expression ?
Answer- following techniques used to quantify gene expression
- RNA Sequencing
- DNA microarray
- RT-PCR
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Which of the following techniques could be used to accurately and precisely quantify gene expression? Select...
Which of the following techniques would allow you to determine whether a gene is alternatively spliced?...
Which of the following techniques would allow you to determine whether a gene is alternatively spliced? a) In situ hybridization b) Northern blot c) Southern blot d) genomic DNA sequencing I honestly need help with this; I first thought it was Northern blot, but I'm starting to second guess myself on that and genomic DNA sequencing. Any help would be appreciated.
For each of the following scenarios, choose which of these recombinant DNA technologies you would use....
For each of the following scenarios, choose which of these recombinant DNA technologies you would use. a) PCR b) cloning vector c) microarray d) in situ hybridization e) reporter genes i. Visualizing the location of a specific gene on the chromosome. _¬_____ ii. Detecting a rare virus in blood. _______ iii. Something in which to insert a gene to synthesize a specific protein. ______ iv. Comparing the gene expression of two different cell types. ______ v. Used to determine the...
Which of the following answer(s) are true (select all that apply)? a)PCR results in exclusively the...
Which of the following answer(s) are true (select all that apply)? a)PCR results in exclusively the desired region to be amplified. b) PCR primers must be outside the region of interest. c) PCR primers must be flanking the region of interest. d) Sequencing primers must be flanking the region of interest. e) All of the nucleotides used in Sanger Sequencing would also be able to be used for a PCR reaction where you are trying to amplify a gene of...
1. Which of the following RNAs can induce gene silencing? tRNA snRNA dsRNA rRNA miRNA 2....
1. Which of the following RNAs can induce gene silencing? tRNA snRNA dsRNA rRNA miRNA 2. Which vehicles are often used for gene therapy to carry a healthy gene? Bacteria Viruses Plastic capsules Both A and B Both A and C 3. A common method in biochemistry to detect and analyze proteins is: Northern blot Southern blot Western blot Eastern blot All of the above 4. The technique used to identify a specific DNA sequence in bacterial colonies is In...
Which of the following are methods employed by cells to control gene expression? (hint: select all...
Which of the following are methods employed by cells to control gene expression? (hint: select all options that apply) Multiple answers: You can select more than one option O A Chromatin remodeling B Histone acetylation O c DNA methylation
Which of the following is a difference between the use of a reporter gene to study...
Which of the following is a difference between the use of a reporter gene to study gene regulation (ex. promoter deletion mapping with a reporter protein) and using a reporter gene to study protein localization? Select all that apply? o When studying protein localization, a fusion protein is created which consists of both the protein of interest and the reporter protein. D Unlike protein localization studies, when studying gene expression, only the reporter gene is expressed and not the actual...
Which of the following accurately describes the promoter of a gene? a) It is the place...
Which of the following accurately describes the promoter of a gene? a) It is the place on the DNA where RNA polymerase binds. b) It determines which DNA strand of a gene is used as a template. c) It is the first region of DNA that is transcribed into RNA. d) both a and b I think the answer is A but I'm not positive. C cannot be correct because the promoter does not get transcribed, and I don't think...
Northern blotting, RT-PCR, and microarrays can be used to analyze gene expression. A lab studies yeast...
Northern blotting, RT-PCR, and microarrays can be used to analyze gene expression. A lab studies yeast cells, comparing their growth in two different sugars, glucose and galactose. One student is comparing expression of the gene HMG2 under these two conditions. Which technique(s) could he use and why? Another student wants to compare expression of all the genes on chromosome 4, of which there are approximately 800. What technique(s) could she use and why?
Sample Question: Which of the following types of gene expression control is unlikely to be used...
Sample Question: Which of the following types of gene expression control is unlikely to be used in bacteria? a) Transcriptional control b) Translational control c) RNA processing control d) Protein activity control
Which of the following is the most appropriate indication of gene expression level? A ONA 6....
Which of the following is the most appropriate indication of gene expression level? A ONA 6. Protein CRNA DmRNA QUESTION 14 In reality, what is being directly quantified in real-time rt-PCR to estimate the gene expression level? OM Protein levels by amplifying with ribosomes using fluorescently tagged amino acids B. DNA which is amplified with gene-specific primers and measured for fluorescence Reverse transcriptase levels which indicates rate of mRNA transcription O mRNA which is amplified with gene-specific primers and measured...
Which of the following are methods employed by cells to control gene expression? (hint: select all options that apply) Multiple answers: You can select more than one option O A Chromatin remodeling B Histone acetylation O c DNA methylation
Which of the following is a difference between the use of a reporter gene to study gene regulation (ex. promoter deletion mapping with a reporter protein) and using a reporter gene to study protein localization? Select all that apply? o When studying protein localization, a fusion protein is created which consists of both the protein of interest and the reporter protein. D Unlike protein localization studies, when studying gene expression, only the reporter gene is expressed and not the actual...
Sample Question: Which of the following types of gene expression control is unlikely to be used in bacteria? a) Transcriptional control b) Translational control c) RNA processing control d) Protein activity control
Which of the following is the most appropriate indication of gene expression level? A ONA 6. Protein CRNA DmRNA QUESTION 14 In reality, what is being directly quantified in real-time rt-PCR to estimate the gene expression level? OM Protein levels by amplifying with ribosomes using fluorescently tagged amino acids B. DNA which is amplified with gene-specific primers and measured for fluorescence Reverse transcriptase levels which indicates rate of mRNA transcription O mRNA which is amplified with gene-specific primers and measured...
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