Question

For each of the following scenarios, choose which of these recombinant DNA technologies you would use. a) PCR b) cloning vector c) microarray d) in situ hybridization e) reporter genes i. Visualizing the location of a specific gene on the chromosome. _¬_____ ii. Detecting a rare virus in blood. _______ iii. Something in which to insert a gene to synthesize a specific protein. ______ iv. Comparing the gene expression of two different cell types. ______ v. Used to determine the function of a regulatory sequence. ______

Answer 1

i. Visualizing the location of a specific gene on the chromosome --- (d). In situ hybridization.

ii. Detecting a rare virus in blood --- (a). PCR.

iii. Something in which to insert a gene to synthesize a specific protein --- (b). Cloning vector.

iv. Comparing the gene expression of two different cell types --- (c). Microarray.

v. Used to determine the function of a regulatory sequence --- (e) reporter genes.