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culture, how would you determine which a mixed Gram negative rm anism was which? Be specific. how 4. If you were given Define

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Lab 20. Selective and Differential Media

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Selective media is the growth media used for the growth of a particular/ any specific/selected microbe to grow. For example a microbe resistant to a particular antibiotic like amoxicillin, in that case, a media will be prepared which contain antibiotic amoxicillin for growth of the particular microbe (resistant to amoxicillin). Amoxicillin will not allow to grow other microbes which are sensitive to this antibiotic.

Another example : if a media is lack of a particular amino acid and that selected microbe can synthesize that amino acid its own, in this case only this selected microbe will grow in this amino acid lacking media and other microbes growth will be restricted.

Differential media is the growth media used to differentiate the microbes in growth media with the help of a special indicator (like phenol red, methylene blue) by using biochemical characteristics of microbes. this type of growth media is used for the detection of microbes and by molecular biologists to detect recombinant strains of a bacteria.

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