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5)In the bacterial genome, only one replication fork can only be present at the time of replication whereas In the human genome, multiple replication fork can be seen at the time of replication.
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5. What is the difference between the replication fork within bacterial and a huma 6. What...
Vocabulary: DNA Replication
Vocabulary: DNA Replication A. Helicase B. Primase C. Single Strand Binding Protein (SSB) D. Topoisomerase E. Origin of Replication F. DNA Polymerase G. Leading Strand H. Lagging strand I. DNA Ligase J. Okazaki Fragment K. Replication Fork L. RNA Primer M. Topoisomerase .1. Site where the replication of a DNA molecule begins. 2. The new continuous complementary DNA strand synthesized in the direction for the replication fork. 3. A discontinuously synthesized DNA strand that elongates in a direction away from the replication fork 4. Relaxes...
DNA Replication. Sketch a replication fork of bacterial DNA in which one strand is being replicated...
DNA Replication. Sketch a replication fork of bacterial DNA in which one strand is being replicated discontinuously and the other is being replicated continuously. List six different enzyme activities associated with the replication process, identity the function of each activity, and show where each would be located on the replication fork. In addition, identify the following features on your sketch: DNA template, RNA primer, Okazaki fragments, and single-stranded DNA binding protein.
5a. For the replication fork shown below: - label the leading and lagging strands; - draw...
5a. For the replication fork shown below: - label the leading and lagging strands; - draw arrows to indicate which direction DNA synthesis is proceeding in for each of these strands; - label their 5' and 3' ends. Replication fork movement →→→ 5'-ATCTGGCAGTACGTACTGGATC CGUCAUGC GTCGAATCTGAC-3' CAGCTTAGACTG-5' ATCTGGCTATTCGT 3'-TAGACCGATAAGCATGACCTAG b. Okazaki fragments are generated during (leading / lagging) strand synthesis (circle the correct answer). c. Some U's are included in one of the strands in the figure. Why are there U's...
Matching the normal function to the enzyme. This enzyme connects Okazaki fragments by forming bonds in...
Matching the normal function to the enzyme. This enzyme connects Okazaki fragments by forming bonds in the A. DNA backbone. Topoisomerase This enzyme opens the DNA helix, separating the strands to expose B. the bases. Primase This enzyme relaxes supercoils in the DNA that form ahead of the replication fork Helicase DNA Polymerase III This enzyme removes primers by cutting out one RNA nucleotide at a D. time and replacing it with DNA. DNA Polymerase This enzyme uses its own...
One major difference in prokaryote replication versus eukaryote replication is a. prokaryotic chromosomes have a single...
One major difference in prokaryote replication versus eukaryote replication is a. prokaryotic chromosomes have a single origin of replication, whereas eukaryotic chromosomes have many. b. prokaryotes have telomeres, and eukaryotes do not. c. the rate of elongation during DNA replication is slower in prokaryotes than in eukaryotes. d. prokaryotic chromosomes have histones, whereas eukaryotic chromosomes do not. e. prokaryotes produce Okazaki fragments during DNA replication, but eukaryotes do not.
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes...
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes the new strand of RNA primase Choose synthesizes the new strand of DNA synthesizes a short fragment of complementary RNA attach to template DNA strands to prevent hydrogen bonding Uncoils the supercoil of prokaryotic chromosomes connects Okazaki fragments of new lagging strand of DNA unwinds and unzips the double stranded DNA Single stranded binding proteins (SSB's) DNA Ligase [Choose] Helicase [Choose) DNA Gyrase (Choose]...
Unwinds DNA strand to make replication fork. Adds free nucleotides to the growing daughter DNA strands...
Unwinds DNA strand to make replication fork. Adds free nucleotides to the growing daughter DNA strands Adds short pieces of RNA to help DNA polymerase start Removes RNA and replaces with DNA Fuses or "glues" fragments of DNA together Proofreads or edits the DNA, checking for mistakes Given the following, DNA Sequence, what is the new daughter strand? (Did you label the 5' and 3' ends?) What is the name of the "fragments" of DNA on the lagging strand after...
a) Suppose you label dividing cells with ^32P. In which DNA molecules will the radioactivity be...
a) Suppose you label dividing cells with ^32P. In which DNA molecules will the radioactivity be incorporated if you were to look during metaphase? b) Suppose you are examining the above cells after replication. In which DNA molecules will the radioactivity be present? In one picture, draw a schematic indicating how DNA is synthesized bi-directionally. The schematic should indicate which are the lagging strands, which are the leading strands, where the RNA primers are, and the 3' and 5' orientation...
In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Open...
In the following diagram, A and B are two Okazaki fragments
generated during DNA replication. Open boxes represent the primers
and dotted lines represent the newly synthesized DNA strands.
a. To which direction (left or right) is the replication fork is
moving? (1 mark)
b. Which Okazaki fragment is made first in the diagram? Explain.
(2 marks)
c. (i) Which enzyme in E. coli synthesizes the primers on
Okazaki fragments? (1 mark)
(ii) What is the difference of this enzyme...
In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Open...
In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Open boxes represent the primers and dotted lines represent the newly synthesized DNA strands. B ---- Template DNA a. To which direction (left or right) is the replication fork is moving? (1 mark) b. Which Okazaki fragment is made first in the diagram? Explain. (2 marks) c. (i) Which enzyme in E. coli synthesizes the primers on Okazaki fragments ? (1 mark) (ii) What is...
DNA Replication. Sketch a replication fork of bacterial DNA in which one strand is being replicated discontinuously and the other is being replicated continuously. List six different enzyme activities associated with the replication process, identity the function of each activity, and show where each would be located on the replication fork. In addition, identify the following features on your sketch: DNA template, RNA primer, Okazaki fragments, and single-stranded DNA binding protein.
5a. For the replication fork shown below: - label the leading and lagging strands; - draw arrows to indicate which direction DNA synthesis is proceeding in for each of these strands; - label their 5' and 3' ends. Replication fork movement →→→ 5'-ATCTGGCAGTACGTACTGGATC CGUCAUGC GTCGAATCTGAC-3' CAGCTTAGACTG-5' ATCTGGCTATTCGT 3'-TAGACCGATAAGCATGACCTAG b. Okazaki fragments are generated during (leading / lagging) strand synthesis (circle the correct answer). c. Some U's are included in one of the strands in the figure. Why are there U's...
Matching the normal function to the enzyme. This enzyme connects Okazaki fragments by forming bonds in the A. DNA backbone. Topoisomerase This enzyme opens the DNA helix, separating the strands to expose B. the bases. Primase This enzyme relaxes supercoils in the DNA that form ahead of the replication fork Helicase DNA Polymerase III This enzyme removes primers by cutting out one RNA nucleotide at a D. time and replacing it with DNA. DNA Polymerase This enzyme uses its own...
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes the new strand of RNA primase Choose synthesizes the new strand of DNA synthesizes a short fragment of complementary RNA attach to template DNA strands to prevent hydrogen bonding Uncoils the supercoil of prokaryotic chromosomes connects Okazaki fragments of new lagging strand of DNA unwinds and unzips the double stranded DNA Single stranded binding proteins (SSB's) DNA Ligase [Choose] Helicase [Choose) DNA Gyrase (Choose]...
Unwinds DNA strand to make replication fork. Adds free nucleotides to the growing daughter DNA strands Adds short pieces of RNA to help DNA polymerase start Removes RNA and replaces with DNA Fuses or "glues" fragments of DNA together Proofreads or edits the DNA, checking for mistakes Given the following, DNA Sequence, what is the new daughter strand? (Did you label the 5' and 3' ends?) What is the name of the "fragments" of DNA on the lagging strand after...
a) Suppose you label dividing cells with ^32P. In which DNA molecules will the radioactivity be incorporated if you were to look during metaphase? b) Suppose you are examining the above cells after replication. In which DNA molecules will the radioactivity be present? In one picture, draw a schematic indicating how DNA is synthesized bi-directionally. The schematic should indicate which are the lagging strands, which are the leading strands, where the RNA primers are, and the 3' and 5' orientation...
In the following diagram, A and B are two Okazaki fragments
generated during DNA replication. Open boxes represent the primers
and dotted lines represent the newly synthesized DNA strands.
a. To which direction (left or right) is the replication fork is
moving? (1 mark)
b. Which Okazaki fragment is made first in the diagram? Explain.
(2 marks)
c. (i) Which enzyme in E. coli synthesizes the primers on
Okazaki fragments? (1 mark)
(ii) What is the difference of this enzyme...
In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Open boxes represent the primers and dotted lines represent the newly synthesized DNA strands. B ---- Template DNA a. To which direction (left or right) is the replication fork is moving? (1 mark) b. Which Okazaki fragment is made first in the diagram? Explain. (2 marks) c. (i) Which enzyme in E. coli synthesizes the primers on Okazaki fragments ? (1 mark) (ii) What is...
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