What happens to the transformed bacterial colonies and effects on the transformation efficiency if the recovery...
What happens to the transformed bacterial colonies and effects on the transformation efficiency if the recovery phase is left out while doing the pGLO transformation of E.coli bacteria?
How does the amount of DNA used in the transformation reaction affect the transformation efficiency?
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What happens to the transformed bacterial colonies and effects
on the transformation efficiency if the recovery...
Results 3. (5 points) Our transformation procedure used 10 u of 0.08 ug/ul PGLO. How does...
Results 3. (5 points) Our transformation procedure used 10 u of 0.08 ug/ul PGLO. How does the concentration of DNA affect the number of colonies and the transformation efficiency? Do you think if we used more concentrated PGLO that we could have gotten a higher transformation efficiency? The numbers of colonies shown below resulted from transformation with 10ul of PGLO at the concentrations shown. a. Calculate the transformation efficiency at each concentration. Express your answers in scientific notation. Write the...
1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
During a particular transformation protocol, you used a stock of PGLO DNA with a concentration of...
During a particular transformation protocol, you used a stock of PGLO DNA with a concentration of 0.08 ug ul. You transformed 191 UL of competent E. coli with 100L of PGLO DNA. You added 191 UL of LB broth to the transformation before plating 100 uL of the culture to a plate labeled +POLO, LB, Amp, Ar. After growing the plates overnight, 136 fluorescent colonies (transformants) were counted. What was the average transformation efficiency of your work?
Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated lab...
Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated labeled Bacteria DNA) 683 (70 ul) 291 (3011) (a) What quantity of DNA (in ng) was used in the transformation? (2 marks) (b) What fraction of the total transformation reaction was plated out? (2 marks) (c) How many transformants (colonies) were there in the total transformation reaction? (2 marks) (d) What was the transformation efficiency, expressed as transformants per ug of DNA...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate). 14) Explain what kinds of bacterial growth you will find on each of 4 plates after incubation ((LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate): under normal light and...
1. How is Chitosan hypothesized to decrease number of bacterial colonies? 2. Through what phenomenon does...
1. How is Chitosan hypothesized to decrease number of bacterial colonies? 2. Through what phenomenon does vertexing and sonication removes bacteria from a suture? 3. What methods tare used to evaluate bacterial concentration in a solution?
gene transfer like conjugatione 5. What is calcium chloride transformation? What does it accomplish? Calcium Chloride...
gene transfer like conjugatione 5. What is calcium chloride transformation? What does it accomplish? Calcium Chloride trensformation increases the ability of a bacteria call to in corporale plasmid DNA which callows it to be genetically frensformed. If accomplishes binding op plasmid DNA IV. 6. Why would you expect to find cell growth on the Luria nutrient agar plate? (LB plate)? 7. Did you expect to find cell growth on the ampicillin Luria plate? Explain WHY or WHY NOT. (LB/amp plate...
1. Descri be the process of a bacterial transformation. Explain how scientists can make bacteria take...
1. Descri be the process of a bacterial transformation. Explain how scientists can make bacteria take up DNA in a laboratory setting. (15 pts.) 2. Genetic transformations are not limited to bacteria. There are real-world applications where scientists have taken genes from one organism and inserted them into another organism. Give an example of how a genetically modified organism (can be any organ- ism, you are not limited to bacteria) have been used to solve real-world issues. Describe the organisms...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
2. What mode of reproduction do bacteria use? Describe a bacterial growth curve. Describe what happens...
2. What mode of reproduction do bacteria use? Describe a
bacterial growth curve. Describe what happens at each step along
the curve. What are some parameters that would affect how the curve
looks? How would you predict the curve would look under different
conditions?
17. Big Red's spot test produced the results shown below: What conclusions can be drawn from these results?
Results 3. (5 points) Our transformation procedure used 10 u of 0.08 ug/ul PGLO. How does the concentration of DNA affect the number of colonies and the transformation efficiency? Do you think if we used more concentrated PGLO that we could have gotten a higher transformation efficiency? The numbers of colonies shown below resulted from transformation with 10ul of PGLO at the concentrations shown. a. Calculate the transformation efficiency at each concentration. Express your answers in scientific notation. Write the...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
During a particular transformation protocol, you used a stock of PGLO DNA with a concentration of 0.08 ug ul. You transformed 191 UL of competent E. coli with 100L of PGLO DNA. You added 191 UL of LB broth to the transformation before plating 100 uL of the culture to a plate labeled +POLO, LB, Amp, Ar. After growing the plates overnight, 136 fluorescent colonies (transformants) were counted. What was the average transformation efficiency of your work?
Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated labeled Bacteria DNA) 683 (70 ul) 291 (3011) (a) What quantity of DNA (in ng) was used in the transformation? (2 marks) (b) What fraction of the total transformation reaction was plated out? (2 marks) (c) How many transformants (colonies) were there in the total transformation reaction? (2 marks) (d) What was the transformation efficiency, expressed as transformants per ug of DNA...
gene transfer like conjugatione 5. What is calcium chloride transformation? What does it accomplish? Calcium Chloride trensformation increases the ability of a bacteria call to in corporale plasmid DNA which callows it to be genetically frensformed. If accomplishes binding op plasmid DNA IV. 6. Why would you expect to find cell growth on the Luria nutrient agar plate? (LB plate)? 7. Did you expect to find cell growth on the ampicillin Luria plate? Explain WHY or WHY NOT. (LB/amp plate...
1. Descri be the process of a bacterial transformation. Explain how scientists can make bacteria take up DNA in a laboratory setting. (15 pts.) 2. Genetic transformations are not limited to bacteria. There are real-world applications where scientists have taken genes from one organism and inserted them into another organism. Give an example of how a genetically modified organism (can be any organ- ism, you are not limited to bacteria) have been used to solve real-world issues. Describe the organisms...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
2. What mode of reproduction do bacteria use? Describe a
bacterial growth curve. Describe what happens at each step along
the curve. What are some parameters that would affect how the curve
looks? How would you predict the curve would look under different
conditions?
17. Big Red's spot test produced the results shown below: What conclusions can be drawn from these results?
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