RECOMBINANT DNA: PLASMID VECTOR engineering is the direct manipulation of an organism's DNA using nology. To begin the recombinant DNA process, scientists must first ide at codes for the production of the protein they want to manufacture. One is to go backwards from the amino acid sequence of the desired protein to ide sequence of the gene. After scientists have identified the gene, they m it. Restriction enzymes or endonucleases from bacterial cells are key in th ia produce restriction...
Explain the use of genetic engineering techniques in analyzing or manipulation DNA (for what are the following processes used) Genetic engineering Electrophoresis Polymerase chain reaction Bacterial transformation DNA sequencing
Why is alkaline SDS used for plasmid DNA isolation but not for genomic DNA isolation?
"In genetic modifications, DNA inserted can come from _____ ." any organism containing the DNA of interest only the same plasmid as the vector only the same species as the vector only the same species as the host only the same phylum as the plasmid vector
Why is genetic engineering such a controversial ethical issue?
Why does cDNA in genetic engineering lack introns?
8) Why wouldn't the introduction of viral DNA via a plasmid to transform a plant also induce a viral infection?
Isolation of genomic DNA follows the same principles as that of obtaining plasmid from E. coli. Which of the following is not included in it?a) Cell lysisb) Removal of proteinsc) Removal of chromosomal DNAd) Dissolving plasmid in water
Describe 2 meiotic sources of recombination? Why is plasmid based engineered DNA considered recombinant?
1. Why do plasmid vectors contain a polylinker? So that the plasmid can be cut into many small fragments to be inserted in the region of interest To allow the plasmid to survive on antibiotic media To find a restriction enzyme site that is useful for inserting the fragment of interest Multiple cut sites are required for the DNA fragment to be inserted. 2.What does blue color mean in blue-white screening with pUC18? The DNA fragment was successfully inserted into...