2. Identify the following components of the prokaryotic gene below (include appropriate numbering on the DNA):...
2. Identify the following components of the
prokaryotic gene below
(include appropriate numbering on the DNA):
a. Pribnow Box
b. -35 region
c. 5’ untranslated region
d. Template strand
e. Nontemplate strand
f. Start site of transcription
Homework Answers
Answer : Components of prokaryotic gene
Pribnow box/TATA box : Pribnow box (TATAAAT) which is present ten base pairs upstream of transcriptional start site (- 10 Sequence )
-35 region ( TTGACG) which is present 35 base pairs upstream of the transcriptional start site
Template strand
Non template strand
Start site of transcription ( which is designated as +1)
Add Answer to:
2. Identify the following components of the
prokaryotic gene below
(include appropriate numbering on the DNA):...
Below is a diagram of a transcription unit and the mRNA made from the transcription unit:...
Below is a diagram of a transcription unit and the mRNA made from the transcription unit: A H TTGACA DNA TATAAT -35 B -10 С D - Start codon Stop codon mRNA 5' 3' E F G Is this a prokaryotic or eukaryotic transcription unit? prokaryotic What is the name of the cofactor required for RNA polymerase to bind to the promoter? Which letters on the above diagram correspond to the following structures? Promoter Pribnow box Non-template strand Transcriptional start...
Below is the partial coding strand of DNA for a gene containing an ORF, shown 5'...
Below is the partial coding strand of DNA for a gene containing
an ORF, shown 5' to 3'. Identify the regions, shown 1 to 9,
associated with the following genetic elements. Some elements may
have more than one associated region.
(a) The promoter region
(b) The ribosome binding site
(c) The ribosome binding site consensus
sequence
(d) The start codon
(f) The expected region for the +1
transcription (where the transcript
begins to be made)
(g) Is this a prokaryotic...
Below is a diagram of a transcription unit and the mRNA made from the transcription unit:...
Below is a diagram of a transcription unit and the mRNA made from the transcription unit: А H DNA TTGACA TATAAT -35 B -10 C D Start codon Stop codon mRNA 5 E F G Is this a prokaryotic or eukaryotic transcription unit? What is the name of the cofactor required for RNA polymerase to bind to the promoter? Which letters on the above diagram correspond to the following structures? Promoters Pribnow box Non-template strand Transcriptional start site- Open Reading...
I. Use the DNA sequence below, which encodes a prokaryotic gene to answer the following questions....
I. Use the DNA sequence below, which encodes a prokaryotic gene to answer the following questions. 11 21 31 41 51 71 81 61 91 CGTAATTGAG GAGGTAGTTG ACGTATGAAT AGTTAACGTA CGGGGGGGAA 101 111 121 131 141 ACCCCCCCTT TTTTTTTTTC GAGCAATAAA AGGGTTACAG ATTGCATGCT a) Write down the corresponding sequences, find them in the sequence above and label them: -35 Consensus sequence: _(label as -35) -10 Consensus sequence (Pribnow box): _ __ (label as Pribnow) Shine-Dalgarno sequence in corresponding mRNA: __ (label as SD)...
please help, thank you! The DNA sequence below represents an edited version of the PISTILLATA gene...
please help, thank you!
The DNA sequence below represents an edited version of the PISTILLATA gene from the model plant Arabidopsis thaliana. The sequence comes from the nontemplate (coding) strand. Use the sequence to answer all questions on this assignment. 9 5'-TTTCTCTCTCTATCTCATCAATGTTACTTTAAAACCAATTTACCTTAATAGGTAAGCTCCTCTTCTTG TTCTTCATATAAATCCACATATCCTCTCCTCCATATCTTAACAATTTCATAGCAAACCCTAAAATTGAGA AAGAGATAGAGAGAGAAAGATGGGTAGAGGAAAGATCGAGATAAAGAGGATAGAGAACGCAAACAA CAGAGTGGTGACGGTATCCTTGCTCTCTTTTTAATCTAAAGTTTCTTTGTCTCAAAAATATTCCATGGC CAAAATCATGTAATAAGTTTTCAATTTCTTTACCAAAAACATGTCAAAAGACCCTTGAATCTTTAACCCT AGATGCAGATAACAACAGGAGATGGGATGATGAGAGATCATGACTCGATTGATCATCGAGATTTTAT AATCTCATCCTGATCAACTCCTATCTATAATATCGTGGTCTTTAGTTTGTCTTTATCAATCTGTGTGTCT TAATCTCAATAAAATATACTCATTAAAATCAAAATCCATAAATACGGACATAAACACGACAGACTTACG -3 0 . What kind of gene features would you expect Arabidopsis thaliana to have, bacterial or eukaryotic? 2. The gene contains a sequence that is recognized by the AP1 transcription...
region is a DNA sequence that regulates transcription. Within a gene, a region is a DNA...
region is a DNA sequence that regulates transcription. Within a gene, a region is a DNA sequence that encodes RNA and a o coding, barcoding o control, coding o noncoding, control o coding, control During transcription, the DNA template is read in the ___direction. o 3 to 5 oC to N terminal ON to C terminal 0 5' to 3' For genes encoding protein, which of the following is the eukaryotic consensus sequence of the promoter? ATAT o GCGC CGCG...
3. Prokaryotic and eukaryotic gene expression compared. Below is an incomplete table of prokaryotic and eukaryotic...
3. Prokaryotic and eukaryotic gene expression compared. Below is an incomplete table of prokaryotic and eukaryotic gene expression in comparison. Fill in the blank using PPT slides, notes and the textbook. Prokaryotic gene expression Eukaryotic gene expression Overview Steps Transcription and translation Yes Transcription and translation coupled? Gene structure No introns Epigenetic modification (chromosome remodeling) transcription, translation, RNA processing, protein processing Transcription in the nucleus and translation in the cytoplasm Interrupted gene with exons and introns RNAPI, II, III Which...
Please solve each item in a detailed and descriptive way. Q5. Total 35 pts. Below given...
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Q5. Total 35 pts. Below given single stranded DNA sequence was retrieved from a prokaryote; Promoter region is shown with yellow color Transcription start site is shown with green color Ribosome binding site is shown with blue color 5'ATAGTCGTCGATCGATGGCTTAGCTAGCTTCGATTTCGTAGCTCTGATTAAACGCGCGCATATATCGAT ATCTAGCTAGCTATATTCGCTGATCGCTAGTGTGCGTGATGCTGCTAGGATCAGGTATCGGTCTGATCTA GTATTAGTGCCCGTAGCTGATGCTTCGTCGTAGATCGCTGATTCGCTAATAGGCTGCTAGTCGATGCTGT A3' A) Write the sequnce of double stranded DNA from given single stranded DNA sequence. (5 pts) B) Show template DNA strand used in transcription. (5 pts) C) Write...
where does transcription begin 3. List the major types of RNA and include what they code...
where does transcription begin
3. List the major types of RNA and include what they code for, their function in the cell and which type is translated. 4. If a bacterial protein has 2,500 amino acids long, how many nucleotide pairs long is the ger sequence that codes for it? 5. Where does transcription begin? 6. What is the template and nontemplate strands of DNA? 7. Why is only one strand transcribed, and is the same strand of DNA always...
6. In the drawings below note and label all important elements (incl.consensus sequences) discussed in lectures...
6. In the drawings below note and label all important elements (incl.consensus sequences) discussed in lectures and tutorial manual and listed below. Prokaryotis operon promoter (-10 and -35 elements), operator, multiple structural renes (for example 3), start site of transcription, start sites of translations, transcription termination sequence Prokaryotic mRNA (polycistronie): transcription start site, multiple ribosome binding sites The Shine-Dalgamo sequence in Ecoli), multiple ORFs (including start and stop codons). transcription termination sequence Eukaryotic genes promoter (TATA box), consensus sequence CAAT,...
Below is a diagram of a transcription unit and the mRNA made from the transcription unit: A H TTGACA DNA TATAAT -35 B -10 С D - Start codon Stop codon mRNA 5' 3' E F G Is this a prokaryotic or eukaryotic transcription unit? prokaryotic What is the name of the cofactor required for RNA polymerase to bind to the promoter? Which letters on the above diagram correspond to the following structures? Promoter Pribnow box Non-template strand Transcriptional start...
Below is the partial coding strand of DNA for a gene containing
an ORF, shown 5' to 3'. Identify the regions, shown 1 to 9,
associated with the following genetic elements. Some elements may
have more than one associated region.
(a) The promoter region
(b) The ribosome binding site
(c) The ribosome binding site consensus
sequence
(d) The start codon
(f) The expected region for the +1
transcription (where the transcript
begins to be made)
(g) Is this a prokaryotic...
Below is a diagram of a transcription unit and the mRNA made from the transcription unit: А H DNA TTGACA TATAAT -35 B -10 C D Start codon Stop codon mRNA 5 E F G Is this a prokaryotic or eukaryotic transcription unit? What is the name of the cofactor required for RNA polymerase to bind to the promoter? Which letters on the above diagram correspond to the following structures? Promoters Pribnow box Non-template strand Transcriptional start site- Open Reading...
I. Use the DNA sequence below, which encodes a prokaryotic gene to answer the following questions. 11 21 31 41 51 71 81 61 91 CGTAATTGAG GAGGTAGTTG ACGTATGAAT AGTTAACGTA CGGGGGGGAA 101 111 121 131 141 ACCCCCCCTT TTTTTTTTTC GAGCAATAAA AGGGTTACAG ATTGCATGCT a) Write down the corresponding sequences, find them in the sequence above and label them: -35 Consensus sequence: _(label as -35) -10 Consensus sequence (Pribnow box): _ __ (label as Pribnow) Shine-Dalgarno sequence in corresponding mRNA: __ (label as SD)...
please help, thank you!
The DNA sequence below represents an edited version of the PISTILLATA gene from the model plant Arabidopsis thaliana. The sequence comes from the nontemplate (coding) strand. Use the sequence to answer all questions on this assignment. 9 5'-TTTCTCTCTCTATCTCATCAATGTTACTTTAAAACCAATTTACCTTAATAGGTAAGCTCCTCTTCTTG TTCTTCATATAAATCCACATATCCTCTCCTCCATATCTTAACAATTTCATAGCAAACCCTAAAATTGAGA AAGAGATAGAGAGAGAAAGATGGGTAGAGGAAAGATCGAGATAAAGAGGATAGAGAACGCAAACAA CAGAGTGGTGACGGTATCCTTGCTCTCTTTTTAATCTAAAGTTTCTTTGTCTCAAAAATATTCCATGGC CAAAATCATGTAATAAGTTTTCAATTTCTTTACCAAAAACATGTCAAAAGACCCTTGAATCTTTAACCCT AGATGCAGATAACAACAGGAGATGGGATGATGAGAGATCATGACTCGATTGATCATCGAGATTTTAT AATCTCATCCTGATCAACTCCTATCTATAATATCGTGGTCTTTAGTTTGTCTTTATCAATCTGTGTGTCT TAATCTCAATAAAATATACTCATTAAAATCAAAATCCATAAATACGGACATAAACACGACAGACTTACG -3 0 . What kind of gene features would you expect Arabidopsis thaliana to have, bacterial or eukaryotic? 2. The gene contains a sequence that is recognized by the AP1 transcription...
region is a DNA sequence that regulates transcription. Within a gene, a region is a DNA sequence that encodes RNA and a o coding, barcoding o control, coding o noncoding, control o coding, control During transcription, the DNA template is read in the ___direction. o 3 to 5 oC to N terminal ON to C terminal 0 5' to 3' For genes encoding protein, which of the following is the eukaryotic consensus sequence of the promoter? ATAT o GCGC CGCG...
3. Prokaryotic and eukaryotic gene expression compared. Below is an incomplete table of prokaryotic and eukaryotic gene expression in comparison. Fill in the blank using PPT slides, notes and the textbook. Prokaryotic gene expression Eukaryotic gene expression Overview Steps Transcription and translation Yes Transcription and translation coupled? Gene structure No introns Epigenetic modification (chromosome remodeling) transcription, translation, RNA processing, protein processing Transcription in the nucleus and translation in the cytoplasm Interrupted gene with exons and introns RNAPI, II, III Which...
Please solve each item in a detailed and descriptive way.
Q5. Total 35 pts. Below given single stranded DNA sequence was retrieved from a prokaryote; Promoter region is shown with yellow color Transcription start site is shown with green color Ribosome binding site is shown with blue color 5'ATAGTCGTCGATCGATGGCTTAGCTAGCTTCGATTTCGTAGCTCTGATTAAACGCGCGCATATATCGAT ATCTAGCTAGCTATATTCGCTGATCGCTAGTGTGCGTGATGCTGCTAGGATCAGGTATCGGTCTGATCTA GTATTAGTGCCCGTAGCTGATGCTTCGTCGTAGATCGCTGATTCGCTAATAGGCTGCTAGTCGATGCTGT A3' A) Write the sequnce of double stranded DNA from given single stranded DNA sequence. (5 pts) B) Show template DNA strand used in transcription. (5 pts) C) Write...
where does transcription begin
3. List the major types of RNA and include what they code for, their function in the cell and which type is translated. 4. If a bacterial protein has 2,500 amino acids long, how many nucleotide pairs long is the ger sequence that codes for it? 5. Where does transcription begin? 6. What is the template and nontemplate strands of DNA? 7. Why is only one strand transcribed, and is the same strand of DNA always...
6. In the drawings below note and label all important elements (incl.consensus sequences) discussed in lectures and tutorial manual and listed below. Prokaryotis operon promoter (-10 and -35 elements), operator, multiple structural renes (for example 3), start site of transcription, start sites of translations, transcription termination sequence Prokaryotic mRNA (polycistronie): transcription start site, multiple ribosome binding sites The Shine-Dalgamo sequence in Ecoli), multiple ORFs (including start and stop codons). transcription termination sequence Eukaryotic genes promoter (TATA box), consensus sequence CAAT,...
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