The protein construct synaptophluorin undergoes an increase in fluorescence when the vesicle that contains the protein...
The protein construct synaptophluorin undergoes an increase in fluorescence when the vesicle that contains the protein construct undergoes exocytosis. What causes this increase in fluorescence? What causes the protein construct to stop fluorescing a minute or two after exocytosis occurs?
Homework Answers
Answer:
SynaptopHluorin is a genetically encoded optical indicator (synaptic transmission reporter) of vesicle release and recycling.
The interiors of synaptic vesicles are acidified (pH 5.7) to keep the synaptopHluorin "off". When vesicle fuses with plasma membrane and release occurs, pH rises to extracellular levels and pH, fluorin protein fluoresces.
SynaptopHluorin spots disappear quickly after stimulation ends because of internalization and re-acidification of endosomes, which quenches synaptopHluorin fluorescence.
Add Answer to:
The protein construct synaptophluorin undergoes an increase in
fluorescence when the vesicle that contains the protein...
5. The hypothetical protein E contains no tyrosine residues and 3 tryptophan residues. When in the...
5. The hypothetical protein E contains no tyrosine residues and 3 tryptophan residues. When in the native folding state, protein E displays maximum emissions at 302 and 330 nm when excited using light at 280 nm. However, when denatured, the observed emission maximum shifts to 350 nm. Please explain briefly why two separate emissions maxima are observed in the native state (address intra- or inter-molecular interactions). Also, please briefly explain why such a shift in emissions occurs upon protein unfolding...
You are interested in the mobility of two different unknown integral plasma membrane proteins—protein X and...
You are interested in the mobility of two different unknown integral plasma membrane proteins—protein X and protein Y—in a cell. In one cell, you fluorescently label all the X proteins. In another cell, you label all the Y proteins. When you perform FRAP analysis on both cells, you find that most of the protein X fluorescence has recovered within 3 minutes, but recovery of the protein Y fluorescence is significantly slower. Even after 10 minutes, only 50% of the protein...
After fertilization but before an embryo implants, it undergoes a period of cell division without an...
After fertilization but before an embryo implants, it undergoes a period of cell division without an increase in size. What is the name of this process and where is the embryo when it occurs? …. Is it mitosis? And why
3) A protein contains an N-terminal cleavable signal sequence and two further transmembrane domains. How is the protein most likely oriented with respect to the ER membrane? How is it oriented when in...
3) A protein contains an N-terminal cleavable signal sequence and two further transmembrane domains. How is the protein most likely oriented with respect to the ER membrane? How is it oriented when introduced to the plasma membrane after trafficking?
22) Before the ribosome translocates during elongation, the ribosome's A site contains a tRNA with the site contains a tRNA without an and the ribosome's P After the ribosome translocate...
22) Before the ribosome translocates during elongation, the ribosome's A site contains a tRNA with the site contains a tRNA without an and the ribosome's P After the ribosome translocates, the A site is over the in the mRNA, the P site contains a tRNA with and the E site contains n the mRNA 23) Termination occurs when the ribosome reaches ay sequence. What two molecules are required for termination to occur? 24) What happens to protein after released from...
on the type of cell resulting in new gene e enters the nucleus and activates specifie...
on the type of cell resulting in new gene e enters the nucleus and activates specifie a step in the regulated the coat b) dynamin binds to the cytosolic e) the adaptor proteins called Sec2seSarl to relea SNAREs 14. The ed assembly of the coat found on a CoPll vesicle is surface of the donor membrane that will form the vesicle d) a Sarl-GEF in the donor membrane ca e) specitie -SNARES in the donor mem that 15. T membrane...
4. The protein shown below is an enzyme-linked receptor. This means that this protein... Circle your...
4. The protein shown below is an enzyme-linked receptor. This means that this protein... Circle your answer a) can speed up chemical reactions. b) can bind to certain signaling molecules. c) all of the above. D. Think about the enzyme-linked receptor we discussed in class. After the signaling molecules binds to this receptor, it causes the active site on the receptor to become available. What binds to this active site? E. Consider the molecule you named in the previous question....
When you drink a cup of milk, what happens to the protein in the milk after...
When you drink a cup of milk, what happens to the
protein in the milk after it has been swallowed? To describe these
processes, you must be able to use the vocabulary
effectively.
FILL IN THE BLANKS
tripeptide is the active form of a digestive enzyme in the stomach that breaks polypeptide chains into smaller acid group polypeptides. dipeptide 2. Cleavage of proteins by pepsin in the stomach results in formation of which get broken down amino acids further in...
1.Describe what happens to a carbon-11 atom when it undergoes positron emission. The decay of a...
1.Describe what happens to a carbon-11 atom when it undergoes
positron emission.
The decay of a carbon-11 atom _____(options;changes a nuetron
into proton, changes proton into neutron, is hit with a neutron,
reconfigures its protons and neutrons) and this causes it to
emit______(options; a negatively charged electron sized particle, a
positively charged electron sized partticle, two atoms and several
neutrons, two neutrons and two protons)
2.An atom undergoes radioactive decay according to this
equation:
___.
What is the identity of...
1.Hemoglobin is a complex protein that contains four polypeptide chains. The normal hemoglobin found in adults...
1.Hemoglobin is a complex protein that contains four polypeptide chains. The normal hemoglobin found in adults –called adult hemoglobin –consists of two alpha and two beta polypeptide chains, which are encoded by different loci. Sickle-cell hemoglobin, which causes sickle-cell anemia, arises from a mutation in the beta chain of adult hemoglobin. Adult hemoglobin and sickle-cell hemoglobindiffer in a single amino acid: the sixth amino acid from one end in adult hemoglobin is glutamic acid, whereas sickle-cell hemoglobin has valine at...
22) Before the ribosome translocates during elongation, the ribosome's A site contains a tRNA with the site contains a tRNA without an and the ribosome's P After the ribosome translocates, the A site is over the in the mRNA, the P site contains a tRNA with and the E site contains n the mRNA 23) Termination occurs when the ribosome reaches ay sequence. What two molecules are required for termination to occur? 24) What happens to protein after released from...
on the type of cell resulting in new gene e enters the nucleus and activates specifie a step in the regulated the coat b) dynamin binds to the cytosolic e) the adaptor proteins called Sec2seSarl to relea SNAREs 14. The ed assembly of the coat found on a CoPll vesicle is surface of the donor membrane that will form the vesicle d) a Sarl-GEF in the donor membrane ca e) specitie -SNARES in the donor mem that 15. T membrane...
4. The protein shown below is an enzyme-linked receptor. This means that this protein... Circle your answer a) can speed up chemical reactions. b) can bind to certain signaling molecules. c) all of the above. D. Think about the enzyme-linked receptor we discussed in class. After the signaling molecules binds to this receptor, it causes the active site on the receptor to become available. What binds to this active site? E. Consider the molecule you named in the previous question....
When you drink a cup of milk, what happens to the
protein in the milk after it has been swallowed? To describe these
processes, you must be able to use the vocabulary
effectively.
FILL IN THE BLANKS
tripeptide is the active form of a digestive enzyme in the stomach that breaks polypeptide chains into smaller acid group polypeptides. dipeptide 2. Cleavage of proteins by pepsin in the stomach results in formation of which get broken down amino acids further in...
1.Describe what happens to a carbon-11 atom when it undergoes
positron emission.
The decay of a carbon-11 atom _____(options;changes a nuetron
into proton, changes proton into neutron, is hit with a neutron,
reconfigures its protons and neutrons) and this causes it to
emit______(options; a negatively charged electron sized particle, a
positively charged electron sized partticle, two atoms and several
neutrons, two neutrons and two protons)
2.An atom undergoes radioactive decay according to this
equation:
___.
What is the identity of...
Most questions answered within 3 hours.
-
could someone please post clear drawings of the three structures
in the equilibrium mixture of D-glucose...
asked 1 minute ago -
Using the Properties of Order show that 5n5 +
4n4 + 6n3 + 2n2+ n +...
asked 5 minutes ago -
What is experiential learning and how is it helpful for teaching
leadership, and interpreting group dynamics?...
asked 4 minutes ago -
A security awareness policy defines the responsibilities of
managers and information owners.
True
False
asked 6 minutes ago -
which is the equilibrium expression for the reaction
3A(g)+4B(g)<---> 2C(g)+5D(g)
asked 7 minutes ago -
Create a balanced compensation plan that you feel would
encourage a restaurant manager to be more...
asked 14 minutes ago -
Re: Human Physiology
Comment on the differences between representing V02 max as an
absolute number and...
asked 17 minutes ago -
A firm with a WACC of 10% is considering the following mutually
exclusive projects:
0
1...
asked 21 minutes ago -
. A 100.0 mL sample of 0.18 M HClO4 is titrated with 0.27 M
LiOH. Determine...
asked 45 minutes ago -
A regression equation that describes the relationship between
the amount of the bill ($) at a...
asked 1 hour ago -
exercise on VSEPR and molecular structrue.
octahedral
SeCl62-
TeCl62-
ClF62-
distorted
SeF62–
IF6–
asked 2 hours ago -
284 mL of a 0.52 M potassium hydroxide solution is added to 467
mL of a...
asked 2 hours ago