How to determine the molecular weight of the BSA, lysozyme and the individual proteins of liver...
Homework Answers
Stained marker is used to estimate the molecular weight of the proteins which are resolved by gel electrophoresis. BSA, lysozyme and the individual proteins of liver antigen along with the stained marker are loaded in to a SDS-PAGE gel and are resolved by electrophoresis. The proteins and the marker are separated in this process. The gel is stained to visualize the bands of the proteins loaded into the gel. After staining, each of the protein band should be matched with the separated marker in order to find the appropriate molecular weight of the protein. (Since the molecular weight of the separated bands of the stained marker is already known)
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How
to determine the molecular weight of the BSA, lysozyme and the
individual proteins of liver...
Proteins: BSA, yeast ADH, ovalbumin, lysozyme . Discuss how you were able to determine which protein...
Proteins: BSA, yeast ADH, ovalbumin, lysozyme
. Discuss how you were able to determine which
protein was in which lane. You will have to do some
online research to find the molecular weight of each protein,
whether it is a dimer, etc. For at least one of the proteins, there
is some variability regarding the molecular weight, but the range
should still allow you to determine which lane each of the proteins
is in. For each lane, pay attention to...
Enzyme Rf Kda BSA 0.16875 66.5 Lysozyme 0.65125 14.3 RNAse A 0.8225 13.7 Enzyme Cm Log...
Enzyme Rf Kda BSA 0.16875 66.5 Lysozyme 0.65125 14.3 RNAse A 0.8225 13.7 Enzyme Cm Log U BSA 1.35 0.16875 Lysozyme 5.21 0.65125 RNAse A 6.58 0.8225 total distance of gel 8 This is the data for the 12% gel acrylamide Enzyme Rf Kda BSA 0.22779043 66.5 Lysozyme 0.6833713 14.3 RNAse A 0.81548975 13.7 Enzyme Cm Log U BSA 1 0.22779043 Lysozyme 3 0.6833713 RNAse A 3.58 0.81548975 total distance of gel 4.39 This is the data for the 15%...
Biochemistry 3. How to analyze the concentration and molecular weight of proteins?
Biochemistry 3. How to analyze the concentration and molecular weight of proteins?
PARTB Experiment 1 (201). Molecular Weight Determination The 1-1. You will be in the wild with...
PARTB Experiment 1 (201). Molecular Weight Determination The 1-1. You will be in the wild with me is found in rabbit serum. This protein is called serumbumin. The sandard unknown proteins and the rabbit serum prins for this experimentarehe pre- tined to allow you to follow the portion during crophews Background Information A first step in the analysis of a protein in the molecular biology laboratory frequently involves determination of its sie or molecular weight. The molecular weight of a...
6. A series of proteins of known mass were running over a Sephacryl S300 column. A....
6. A series of proteins of known mass were running over a Sephacryl S300 column. A. Determine the molecular mass of the unknown protein. B. Give a plausible explanation for the strange behavior of ferritin’s elution from the column. How would you test your idea? Protein Mr (kDa) Ve (mL) Blue dextran 1000 85 Lysozyme 14 200 Chymotrypsinogin 25 190 Ovalbumin 45 170 BSA 65 150 Aldolase 150 125 Urease 500 90 Ferritin 700 92 Ovomucoid 28 160 unknown ??...
Molecular weight data for some polymer are tabulated. Determine: a) The number-average molecular weight b) The...
Molecular weight data for some polymer are tabulated.
Determine:
a) The number-average molecular weight
b) The weight-average molecular weight
c) What is the number-average degree of polymerization for this
polymer?
d) What is the weight-average degree of polymerization for this
polymer?
Side note: Please
consider the monomer C3H6
Molecular weight data for some polymer are tabulated. Determine: The number-average molecular weight The weight-average molecular weight What is the number-average degree of polymerization for this polymer? What is the weight-average degree...
Design a possible purification procedure to purify lysozyme (pI = 11.35 and molecular mass = 14.3 kDa) from egg white. H...
Design a possible purification procedure to purify lysozyme (pI = 11.35 and molecular mass = 14.3 kDa) from egg white. How are you going to evaluate purity and activity?
Assume you were given a mixture consisting of one molecule each of all possible sequences of a smallish protein of molecular weight 4800 daltons. If the average molecular weight of an amino acid is, s...
Assume you were given a mixture consisting of one molecule each of all possible sequences of a smallish protein of molecular weight 4800 daltons. If the average molecular weight of an amino acid is, say, 120 daltons, how much would the sample weigh? How big a container would you need to hold it? What does this calculation tell you about the fraction of possible proteins that are currently in use by living organisms (the average molecular weight of proteins is...
Which technique could be used for molecular weight determinations in proteins under all circumstances? a.) gel...
Which technique could be used for molecular weight determinations in proteins under all circumstances? a.) gel filtration b.) isoelectric focusing c.) carboxymethyl (CM) cellulose chromatography d.) a and e e.) SDS-PAGE
5. If you loaded 0.2 ug of molecular weight markers with the following sized bands, how...
5. If you loaded 0.2 ug of molecular weight markers with the following sized bands, how many ng of DNA would be in the 0.5 kB band? Circle your answer and include units. 14 kB 5 kB 500 bp 4.5 kB 2000 bp 24 kB 2.3 KB 5050 bp How many ng would will be in the top band?
Proteins: BSA, yeast ADH, ovalbumin, lysozyme
. Discuss how you were able to determine which
protein was in which lane. You will have to do some
online research to find the molecular weight of each protein,
whether it is a dimer, etc. For at least one of the proteins, there
is some variability regarding the molecular weight, but the range
should still allow you to determine which lane each of the proteins
is in. For each lane, pay attention to...
PARTB Experiment 1 (201). Molecular Weight Determination The 1-1. You will be in the wild with me is found in rabbit serum. This protein is called serumbumin. The sandard unknown proteins and the rabbit serum prins for this experimentarehe pre- tined to allow you to follow the portion during crophews Background Information A first step in the analysis of a protein in the molecular biology laboratory frequently involves determination of its sie or molecular weight. The molecular weight of a...
Molecular weight data for some polymer are tabulated.
Determine:
a) The number-average molecular weight
b) The weight-average molecular weight
c) What is the number-average degree of polymerization for this
polymer?
d) What is the weight-average degree of polymerization for this
polymer?
Side note: Please
consider the monomer C3H6
Molecular weight data for some polymer are tabulated. Determine: The number-average molecular weight The weight-average molecular weight What is the number-average degree of polymerization for this polymer? What is the weight-average degree...
5. If you loaded 0.2 ug of molecular weight markers with the following sized bands, how many ng of DNA would be in the 0.5 kB band? Circle your answer and include units. 14 kB 5 kB 500 bp 4.5 kB 2000 bp 24 kB 2.3 KB 5050 bp How many ng would will be in the top band?
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