Homework Answers
6) The answer is b.It is basically involved in packaging of fat.It serves as a carrier for cholesterol into the brain.
7) The answer is a.As according to the gene of interest primers are designed using bioinformatics tools.and two primers one is forward other is reverse are required.
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6. The APOE protein is a type of protein that Regulates transcription (a transcription factor) Is...
You are using a ChIP assay to study the positioning of a DNA-binding transcription factor (Dbp1p)...
You are using a ChIP assay to study the positioning of a
DNA-binding transcription factor
(Dbp1p) on transcribed genes. You fragment DNA from wild type
cells and immunoprecipitate
Dbp1p with an antibody to it. Then you PCR amplify the DNA
associated with Dbp1p using
different sets of primers for a particular gene (G3PD). One set
of primers was specific for the
TATA box region of this gene, (lane 1) another pair of primers
amplified the 3’ end of the open...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using blue/white colony selection, why could you assume that white colonies have modified plasmids? a. A blue colony means the LacZ reading-frame was disrupted b. A blue colony means your gene has mutations c. A white colony means the LacZ reading-frame is intact d. A white colony means the LacZ reading-frame was disrupted QUESTION 2: You are performing a PCR using primers with a sequence perfectly...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance of trp B. The trp operon would be transcribed much less than...
Question 1: Saved Advantages of the Polymerase Chain Reaction include all of these, except: 1) The...
Question 1: Saved Advantages of the Polymerase Chain Reaction include all of these, except: 1) The reaction is specific for certain sequences in the DNA. 2) Only small amounts of template are needed. 3) Results can only be obtained with freshly isolated DNA. 4) All the products from a specific part of the DNA will be the same size. Question 2: Saving The chain terminator used in Sanger's DNA sequencing is works due to.. 1) lack of 2'OH 2) ddNTP:...
Yet, all the cells in your body contain the same genes (and same alleles). The difference...
Yet, all the cells in your body contain the same
genes (and same alleles). The difference across cell types
is that genes get selectively expressed (turned on or off)
based on the proteins needed for cellular function given their
environment.
Select which statement explains the reason why hair does not
normally grow on your muscle cells.
a.
Muscle cells have the gene for keratin, but do not express
it
b.
Muscle cells do not have the gene for keratin and...
A cell's genome is its blueprint for life. However, what is the bare minimum number of...
A cell's genome is its blueprint for life. However, what is the bare minimum number of genes needed to sustain a free-living cell? This is a question that microbiologists at the J. Craig Venter Institute (JCVI) have attempted to answer ever since they sequenced the genomes of several Mycoplasma species in the 1990s. Because Mycoplasma species are parasitic bacteria, their genomes are already reduced in size and hence provide an excellent foundation for creating a "minimal cell." However, little did...
Page < 2 > of 2 D. The trp operon would be expressed at high levels,...
Page < 2 > of 2 D. The trp operon would be expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration;...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...
You are using a ChIP assay to study the positioning of a
DNA-binding transcription factor
(Dbp1p) on transcribed genes. You fragment DNA from wild type
cells and immunoprecipitate
Dbp1p with an antibody to it. Then you PCR amplify the DNA
associated with Dbp1p using
different sets of primers for a particular gene (G3PD). One set
of primers was specific for the
TATA box region of this gene, (lane 1) another pair of primers
amplified the 3’ end of the open...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance of trp B. The trp operon would be transcribed much less than...
Yet, all the cells in your body contain the same
genes (and same alleles). The difference across cell types
is that genes get selectively expressed (turned on or off)
based on the proteins needed for cellular function given their
environment.
Select which statement explains the reason why hair does not
normally grow on your muscle cells.
a.
Muscle cells have the gene for keratin, but do not express
it
b.
Muscle cells do not have the gene for keratin and...
Page < 2 > of 2 D. The trp operon would be expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...
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