Question

Give step-by-step instructions on how to make a serial dilution with a factor of 1:50. Give instructions for 5 concentrations, including a control. (concentrations were not given.)

Answer 1

The first step in making a serial dilution is to take a known volume (usually 1ml) of stock and place it into a known volume of distilled water (usually 9ml). This produces 10ml of the dilute solution. This dilute solution has 1ml of extract /10ml, producing a 10-fold dilution.

Now, If we want to make a 1/50 dilution we add 1 volume part of the one to 49 parts of the other, to make up 50 parts in all.

Let we make 50ml solution of NaOH with a factor of 1/50 then- we should add 1ml of NaOH and 49ml of water to make the solution.

Similarly if we r making 100ml of KOH solution with water by 1/50 factor then we should add 2ml of KOH to 98ml of water.