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diagram a 3 fold serial dilution series out to a 1:243 dilution. include the dilution at...

diagram a 3 fold serial dilution series out to a 1:243 dilution. include the dilution at each step.

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c_1v_1 = c_2v_2

Since we need to have the difference in concentration of 1:243 and need to use 3 folder dilution. Hence, number of dilution stages will be

243 = 3^n

3^5 = 3^n, n = 5

Hence there will be five dilution stages in order to reach 1:243 concentration using 3-fold dilution

Dilution of Individual Step 1/3 1/3 1/3 1/3 1/3
DF of individual step 3 3 3 3 3
Total Dilution after each step 1/3 1/9 1/27 1/81 1/243
Total DF after each step 3 9 27 81 243

The diagram is be given below

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