diagram a 3 fold serial dilution series out to a 1:243 dilution. include the dilution at each step.
Please dont forget to rate the answer as thumbs up
Since we need to have the difference in concentration of 1:243 and need to use 3 folder dilution. Hence, number of dilution stages will be
Hence there will be five dilution stages in order to reach 1:243 concentration using 3-fold dilution
Dilution of Individual Step | 1/3 | 1/3 | 1/3 | 1/3 | 1/3 |
DF of individual step | 3 | 3 | 3 | 3 | 3 |
Total Dilution after each step | 1/3 | 1/9 | 1/27 | 1/81 | 1/243 |
Total DF after each step | 3 | 9 | 27 | 81 | 243 |
The diagram is be given below
diagram a 3 fold serial dilution series out to a 1:243 dilution. include the dilution at...
7. What is the final dilution factor of a 4 fold serial dilution with 5 total tubes including the "neat" tube? (neat means undiluted or the original specimen) 1:4 2 Neat ? Dilution factor 7. What is the final dilution factor of a 4 fold serial dilution with 5 total tubes including the "neat" tube? (neat means undiluted or the original specimen) 1:4 2 Neat ? Dilution factor
1. Make a 10 fold serial dilution of a protein to achieve a final concentration of 10ug/ml You are staring from a 10 mg/ml protein stock. Indicate the amount of sample needed (from previous tube) and the amount of H20 needed. Make your calculations for 100μ1 volume. (0.5pts) 10ul 90μ1 H20 10mg/ml lmg/ml
#1) What should be the concentration of kanamycin if a serial 2-fold dilution was done starting with tube 1 and ending in tube 9 with an initial concentration of 100 ug/mL tube # 1 2 3 4 5 6 7 8 9 10 [control] Kanamycin concentration (ug/ml) 100 ? ? ? ? ? ? ? ? 0 growth no no no no no yes yes yes yes yes #2) Culture C has a titer of 2.3 x 107 bacteria/mL. Draw...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
You need to do a four-step serial dilution from a urine sample. The end volume for this 10-fold dilution is 3,000ul. Draw and label (amount of sample, amount of diluent, and dilution factor). (4 points)
Serial dilution problem You have a stock of 5% SDS. You take some of that stock and dilute it 4-fold in tube #1. the tubes hold 1.5ml. give an example of how much 5% stock and how much water you would use to make 4 fold dilution - volume of 5% stock ? - volume of water? - How much volume transferred? - if you took a sample out of tube 2 and did another 4 fold dilution, what concentration...
Give an example of a serial dilution, provide a diagram and calculation to demonstrate your understanding of a serial dilution
You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies. a) What was the dilution factor? b) How many bacteria were present in the soil?
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100, and 1/10. Then you plate 100 µl of the final dilution and the next day observe 200 colonies on your plate. What was the concentration of the original culture? (5 pts)
Serial Dilution Questions 1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2,00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of...