You need to do a four-step serial dilution from a urine sample. The end volume for...

Question

Question

You need to do a four-step serial dilution from a urine sample. The end volume for this 10-fold dilution is 3,000ul. Draw and

Answer 1

Answer #1

QnO/ MED24础。 s品 3 4。 300 30 +3 on to (30mL, =3M/

Please rate high.

Answer 2

Similar Homework Help Questions

Serial dilution problem You have a stock of 5% SDS. You take some of that stock...

Serial dilution problem You have a stock of 5% SDS. You take some of that stock and dilute it 4-fold in tube #1. the tubes hold 1.5ml. give an example of how much 5% stock and how much water you would use to make 4 fold dilution - volume of 5% stock ? - volume of water? - How much volume transferred? - if you took a sample out of tube 2 and did another 4 fold dilution, what concentration...
1) if 5 parts sample is added to 10 parts diluents, what is the dilution that...

1) if 5 parts sample is added to 10 parts diluents, what is the dilution that was just made? 2. What is the ratio if 5 parts sample volume is added to 10 parts diluents volume? 3. In a 1 to 3 dilution, what is the dilution FACTOR? 4. In a 1 to 10 dilution, what is the dilution FACTOR? 5. What is the dilution FACTOR if 40 mcL of sample added to 260 mcL of diluent? 6. What is...
1. Make a 10 fold serial dilution of a protein to achieve a final concentration of...

1. Make a 10 fold serial dilution of a protein to achieve a final concentration of 10ug/ml You are staring from a 10 mg/ml protein stock. Indicate the amount of sample needed (from previous tube) and the amount of H20 needed. Make your calculations for 100μ1 volume. (0.5pts) 10ul 90μ1 H20 10mg/ml lmg/ml

3. A serial dilution of a swab sample was prepared by adding 10 uL to a...

3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
Draw out the serial dilution you will need to make using an original concentration of 10^8...

Draw out the serial dilution you will need to make using an original concentration of 10^8 and the Total Dilution Factors as 2, 4, 8, and 16.
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....

Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...

You have performed a serial dilution of an unknown sample and counted 73 CFU on a...

You have performed a serial dilution of an unknown sample and counted 73 CFU on a countable plate that was marked as a 10-4 dilution and you used 0.1ml to inoculate the plate. What is the population density of the original sample? Please Show Work.....
You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial...

You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies. a) What was the dilution factor? b) How many bacteria were present in the soil?
I have to do a serial dilution for plating bacteria, and I am given a sample...

I have to do a serial dilution for plating bacteria, and I am given a sample that has been diluted to 10-4 already. I will need to plate the 10-5 and 10-7 dilutions. What will be the steps I will have to do? Note: I cannot use any more tubes/saline outside of what I have been given. Materials: 2 tubes labelled 10-5 and 10-7. 20ml of sterile saline 5 ml of the 10-4 diluted sample. Sterile pipettes

You have performed a serial dilution of a sample with an unknown concentration of microorganisms. You...

You have performed a serial dilution of a sample with an unknown concentration of microorganisms. You counted 55 CFU on a countable plate inoculated with 0.1 mL of a 10-3 dilution. What was the population density of the original sample per milliliter? Many of the quantitative methods discussed are popular because they enable the microbiology researcher to selectively count live cells only. Why do you think this might be an important or desirable feature in a medical or environmental research...