Homework Answers
The primer that will bind to the lower strand would be 5'AAGGC 3' and the primer that will bind to the upper DNA strand would be 5'CCTTT 3' due to complementarity of the bases.
So option number 8 and 5 are correct.
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Choose the two primers from the list that will amplify the region indicated by the bracket....
1. Explain why, when PCR is used to amplify the same region of DNA from two...
1. Explain why, when PCR is used to amplify the same region of DNA from two different people, the size of the DNA fragment(s) generated may be different? 2. What characteristic of the DNA molecule makes it possible to use electrophoresis to separate DNA molecules by size? Explain why this characteristic is important for electrophoresis and what part of the DNA molecule creates this characteristic. 3. You are performing PCR. After four cycles of PCR, how many double-stranded copies of...
EXPLAIN each answer thoroughly. There are two common goals when using PCR to amplify DNA. A....
EXPLAIN each answer thoroughly. There are two common goals when using PCR to amplify DNA. A. Make lots of copies of a specific DNA sequence to use in cloning (preparative PCR) B. Detect the presence or relative amount of a specific gene under varying conditions (analytical PCR) *Remember: PCR is very similiar to DNA replication, but uses a DNA polymerase to amplify only specific parts of DNA sequence based on the sequence of the primers. 3. For which goal (A...
You want to amplify the DNA between the two stretches of sequence shown below. Of the...
You want to amplify the DNA between the two stretches of sequence shown below. Of the listed primer pairs, choose the correct pair that will allow you to amplify the DNA by PCR. 5'-ACATTACCAA----GACCTGCTTT-3' 3' -TGTAATGGTT----CTGGACGAAA-5' 5' -TGTAATGGTT-3' and 5'-TTTCGTCCAG-3' O 5'-ACATTACCAA-3' and 5'-AAAGCAGGTC-3' 5'-TGTAATGGTT-3' and 5'-CTGGACGAAA-3' O 5'-AACCATTACA-3' and 5'-CTGGACGAAA-3'
You want to amplify the DNA between the two stretches of sequence shown below. Of the...
You want to amplify the DNA between the two stretches of sequence shown below. Of the listed primer pairs, choose the correct pair that will allow you to amplify the DNA by PCR. 5’-CTGGACGAAA----TGTAATGGTT-3’ 3’-GACCTGCTTT----ACATTACCAA-5’ A. 5’-CTGGACGAAA-3’ and 5’-TGTAATGGTT-3’ B. 5’-CTGGACGAAA-3’ and 5’-AACCATTACA-3’ C. 5’-TTTCGTCCAG-3’ and 5’-TGTAATGGTT-3’ D. 5’-GACCTGCTTT-3’ and 5’-ACATTACCAA-3’
Select two primers from the list below such that your selected primers create a pair capable...
Select two primers from the list below such that your selected primers create a pair capable of producing a PCR product. To help, we've bolded and underlined the regions where your primers will bind. 5 '-tcgccgtgccgtgcttagcgccgctc. . .tacactgtaccggtacagttcccggg-3 3'-agcggcacggcacgaatcgcggcgag...atgtgacatggccatgtcaagggccc-5' Select all that apply: a. 5'-tgccgtgctt-3 cross out cross out О с.5'-acggcacgaa-3, cross out d. 5'-aagcacggca-3 cross out cross out e. 5'-cggtacagtt-3" f.5'-ttgacatggc-3 cross out cross out cross out h. 5'-aactgtaccg-3
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
1) Imagine that you are interested in studying the enzyme dihydrofolate reductase (DHFR) from Mycobacterium tuberculosis...
1) Imagine that you are interested in studying the enzyme dihydrofolate reductase (DHFR) from Mycobacterium tuberculosis (this enzyme is a potential drug target). The amino acid sequence of the protein is: MTMVGCIWAQATSGVIGRGGDIPWRLPEDQAHFREITMGHTIVMGRRTWDSLPAKVRPLPGRRNWL SRQADFMASGAEWGSLEEALTSPETWVIGGGQVYALALPYATRCEVTEVDIGLPREAGDALAPVLD ETWRGETGEWRFSRSGLRYRLYSYHRS The DNA sequence within the M. tuberculosis genome that codes for the protein is: ATGACGATGGTGGGGCTGATCTGGGCTCAAGCGACATCGGGTGTCATCGGCCGCGGCGGCGACATCCCCT GGCGCTTGCCCGAGGACCAGGCGCATTTCCGGGAGATCACCATGGGGCACACGATCGTGATGGGCCGGCG CACATGGGATTCGCTGCCGGCTAAAGTCCGGCCGCTGCCCGGCCGGCGAAATGTCGTACTGAGCCGCCAA GCTGACTTTATGGCCAGCGGGGCTGAGGTTGTCGGTTCACTCGAGGAGGCGCTGACCAGCCCGGAGACGT GGGTGATCGGAGGCGGACAAGTCTATGCGCTGGCGCTGCCGTACGCGACCAGATGTGAGGTTACCGAGGT CGACATCGGCCTGCCGCGCGAAGCCGGTGACGCGCTGGCCCCCGTGCTGGACGAGACATGGCGGGGCGAG ACGGGGGAGTGGCGCTTCAGCCGGTCCGGGTTGCGGTACCGGTTGTACAGCTACCACCGCTCATGA Assume that you have been given a sample of the bacterial genomic DNA, restriction enzymes Ndel and BamHi, and a plasmid from Novagen called PET-28b....
e) (2 points) Your supervisor suggests you produce a cDNA library for the yeast in order...
e) (2 points) Your supervisor suggests you produce a cDNA library for the yeast in order to produce the genes in bacteria and perform enzymatic analysis. You do so and are able to isolate a vector for your gene (you call this gene Yuml). You send your vector in for sequencing and are given the sequence of your gene and surrounding regions (shown below). 5' TCCGGCGGAATTCCAAGGCCT 3' AGGCCGCCTTAAGGTTCCGGA Yum1 CGTCGACTCCGGC GCAGCTGAGGCCG 3' 5' You want to PRC amplify your specific...
1. The following DNA sequence was discovered in a metagenomic analysis of a soil sample. It...
1. The following DNA sequence was discovered in a metagenomic analysis of a soil sample. It is 249 bp long, and is suspected to be a serine protease inhibitor ATGAGCAGCGGCGGCCTGCTGCTGCTGCTGGGCCTGCTGACCTTTTGCGCGGAACTGACC CCGGTGAGCAGCCGCAAACGCCATCCGTATTGCAACCTGCCGCCGGATCCGGGCCCGTGC CATGATAACAAATTTGCGTTTTATCATCATCCGGCGAGCAACAAATGCAAAGAATTTGTG TATGGCGGCTGCGGCGGCAACGATAACCGCTTTAAAACCCGCAACAAATGCCAGTGCACC TGCAGCGGC a) Which sequencing method would you use to sequence a gene in this size range, and why? (Name of technique and 1-2 sentence explanation.) b) What technique would you use to amplify the DNA, in order to make more of it for future experiments? (What is...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using blue/white colony selection, why could you assume that white colonies have modified plasmids? a. A blue colony means the LacZ reading-frame was disrupted b. A blue colony means your gene has mutations c. A white colony means the LacZ reading-frame is intact d. A white colony means the LacZ reading-frame was disrupted QUESTION 2: You are performing a PCR using primers with a sequence perfectly...
You want to amplify the DNA between the two stretches of sequence shown below. Of the listed primer pairs, choose the correct pair that will allow you to amplify the DNA by PCR. 5'-ACATTACCAA----GACCTGCTTT-3' 3' -TGTAATGGTT----CTGGACGAAA-5' 5' -TGTAATGGTT-3' and 5'-TTTCGTCCAG-3' O 5'-ACATTACCAA-3' and 5'-AAAGCAGGTC-3' 5'-TGTAATGGTT-3' and 5'-CTGGACGAAA-3' O 5'-AACCATTACA-3' and 5'-CTGGACGAAA-3'
Select two primers from the list below such that your selected primers create a pair capable of producing a PCR product. To help, we've bolded and underlined the regions where your primers will bind. 5 '-tcgccgtgccgtgcttagcgccgctc. . .tacactgtaccggtacagttcccggg-3 3'-agcggcacggcacgaatcgcggcgag...atgtgacatggccatgtcaagggccc-5' Select all that apply: a. 5'-tgccgtgctt-3 cross out cross out О с.5'-acggcacgaa-3, cross out d. 5'-aagcacggca-3 cross out cross out e. 5'-cggtacagtt-3" f.5'-ttgacatggc-3 cross out cross out cross out h. 5'-aactgtaccg-3
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
1) Imagine that you are interested in studying the enzyme dihydrofolate reductase (DHFR) from Mycobacterium tuberculosis (this enzyme is a potential drug target). The amino acid sequence of the protein is: MTMVGCIWAQATSGVIGRGGDIPWRLPEDQAHFREITMGHTIVMGRRTWDSLPAKVRPLPGRRNWL SRQADFMASGAEWGSLEEALTSPETWVIGGGQVYALALPYATRCEVTEVDIGLPREAGDALAPVLD ETWRGETGEWRFSRSGLRYRLYSYHRS The DNA sequence within the M. tuberculosis genome that codes for the protein is: ATGACGATGGTGGGGCTGATCTGGGCTCAAGCGACATCGGGTGTCATCGGCCGCGGCGGCGACATCCCCT GGCGCTTGCCCGAGGACCAGGCGCATTTCCGGGAGATCACCATGGGGCACACGATCGTGATGGGCCGGCG CACATGGGATTCGCTGCCGGCTAAAGTCCGGCCGCTGCCCGGCCGGCGAAATGTCGTACTGAGCCGCCAA GCTGACTTTATGGCCAGCGGGGCTGAGGTTGTCGGTTCACTCGAGGAGGCGCTGACCAGCCCGGAGACGT GGGTGATCGGAGGCGGACAAGTCTATGCGCTGGCGCTGCCGTACGCGACCAGATGTGAGGTTACCGAGGT CGACATCGGCCTGCCGCGCGAAGCCGGTGACGCGCTGGCCCCCGTGCTGGACGAGACATGGCGGGGCGAG ACGGGGGAGTGGCGCTTCAGCCGGTCCGGGTTGCGGTACCGGTTGTACAGCTACCACCGCTCATGA Assume that you have been given a sample of the bacterial genomic DNA, restriction enzymes Ndel and BamHi, and a plasmid from Novagen called PET-28b....
e) (2 points) Your supervisor suggests you produce a cDNA library for the yeast in order to produce the genes in bacteria and perform enzymatic analysis. You do so and are able to isolate a vector for your gene (you call this gene Yuml). You send your vector in for sequencing and are given the sequence of your gene and surrounding regions (shown below). 5' TCCGGCGGAATTCCAAGGCCT 3' AGGCCGCCTTAAGGTTCCGGA Yum1 CGTCGACTCCGGC GCAGCTGAGGCCG 3' 5' You want to PRC amplify your specific...
1. The following DNA sequence was discovered in a metagenomic analysis of a soil sample. It is 249 bp long, and is suspected to be a serine protease inhibitor ATGAGCAGCGGCGGCCTGCTGCTGCTGCTGGGCCTGCTGACCTTTTGCGCGGAACTGACC CCGGTGAGCAGCCGCAAACGCCATCCGTATTGCAACCTGCCGCCGGATCCGGGCCCGTGC CATGATAACAAATTTGCGTTTTATCATCATCCGGCGAGCAACAAATGCAAAGAATTTGTG TATGGCGGCTGCGGCGGCAACGATAACCGCTTTAAAACCCGCAACAAATGCCAGTGCACC TGCAGCGGC a) Which sequencing method would you use to sequence a gene in this size range, and why? (Name of technique and 1-2 sentence explanation.) b) What technique would you use to amplify the DNA, in order to make more of it for future experiments? (What is...
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