Homework Answers
Transformed cells contains the gene for amphicillin resistant and can grow in all medium. While non transformed cells will die if amphicillin is present because they do not carry gene for transformed cells.
Ans-9- option A (LBA)
Ans-10- option D (both B and C)
Ans-11- option A (LBA)
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9. On which of the following plate, both transformed and non-transformed cells grow? A. LBAB.LBA+amp C....
12. If you used the PBLU plasmid as a vector to carry a desirable human gene...
12. If you used the PBLU plasmid as a vector to carry a desirable human gene into E. coli recipient cells. The insertion site is inside the B-galactosidase gene on the p-BLU plasmid. After the transformation, you plate the cells on LBA+amp+X-Gal to screen for E. coli cells with the desirable gene. You saw both blue and white colonies on the plate. Which type of colonies carry the desirable human gene? A. White B. Blue C. both D. neither one...
3. Name a procedure which can replace the heat shock process and achieve the same goal:...
3. Name a procedure which can replace the heat shock process and achieve the same goal: 4. What is the purpose of adding ampicillin to LBA? A. LBA prevents contamination of the plates. B. Ampicillin is a growth factor that stimulates growth of E. coli cells. C. Ampicillin inhibits growth of E. coli cells that do not have amp gene. D. Ampicillin inhibits growth of E. coli cells harboring PBLU plasmid. 5. When B-galactosidase is present, X-Gal turns A. white...
1. On which plate(s) would you expect to find bacteria most like the E. coli on...
1. On which plate(s) would you expect to find bacteria most like the E. coli on the source plate? Explain. 2. On which plate(s) would you find only genetically transformed bacterial cells? Why? 3. What is the purpose of the control plates? Explain the difference between the controls and why each one is necessary. 4. Why would one compare the -DNA/+Amp and DNA/+Amp plates?
number 2 Plasmid-small extrachromosomal dsDNA which contains genes that regulate non-essential life functions Transformation-acquired genes from...
number 2
Plasmid-small extrachromosomal dsDNA which contains genes that regulate non-essential life functions Transformation-acquired genes from free-floating DNA in the environment Competent cell-bacterial cell wall and cytoplasmic membrane become porous and allows transforming DNA to enter the cell 2. Explain why-control E. coli cells cannot grow on L.BA+amp and L.BA+amp+X-Gal. 3. goal: Name a procedure which can replace the heat shock process and achieve the same electroportation What in the ninnee of andinn amnicillin to I RA2
9. All activated 1ymphocytes become.... a. plasma cells b. effector cells. c. memory cells. d. cytotoxic...
9. All activated 1ymphocytes become.... a. plasma cells b. effector cells. c. memory cells. d. cytotoxic cells e. effector and memory cellsob 10. The bacterial lung intection acquired by inhalation ot aerosols of contasinates water is known as M. a. whooping cough. b. walking preumonia. c. influenza. d. Legionnaires' disease 11. Mycobacteriun leprae is only grown in living cella. 5. Th a. True b. False 12. which of the following agento travel up the nerves to penetrate the cs a....
E. coli cells were spread on two agar plates [one containing only agar (plate 1) and...
E. coli cells were spread on two agar plates [one containing only agar (plate 1) and the second containing streptomycin (plate 2) which kills all cells except those that are resistant mutants]. Both plates were allowed to grow for several generations. Cells were collected from the agar plate WITHOUT streptomycin and spread again on a fresh agar plate containing streptomycin (plate 3). More mutants were observed on plate 3 than were observed on plate 2. The experiment demonstrates A. how...
Question 10 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of...
Question 10 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of arabinose on the expression of GFP, predict what will happen when we plate bacteria that are TRANSFORMED on plates containing ampicillin, but NO arabinose. the bacteria will grow and glow the bacteria will grow, but won't glow the bacteria will not grow Question 11 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of arabinose on the expression of GFP,...
c. Lederberg & Tatum 23. A culture of a coli susceptible to ampicillin is transformed with...
c. Lederberg & Tatum 23. A culture of a coli susceptible to ampicillin is transformed with a plasmid that conta ampiellin-resistance gene plus a gene of interest, c.g. the gene for the female hormone, estrogen coll is placed on a growth medium containing ampicillin, then a. no bacteria will grow. b, only ampicillin-sensitive bacteria will grow. c. only bacteria containing the plasmid will grow. d. all bacteria will grow. c. none of these the flavinanses except
2. Which of the following cells are leukocytes? a. NK cells b. neutrophils c. monocytes d....
2. Which of the following cells are leukocytes? a. NK cells b. neutrophils c. monocytes d. all of the above e. none of the above 3 Which of the following cells are lymphocytes? a. NK cells b. neutrophils c. monocytes d. all of the above e. none of the above 4. Why do complement proteins not damage healthy cells normally? a. Complement proteins must be produced by activated leukocytes. b. Complement proteins must be activated by enzymatic cleavage. c. Leukocytes...
49. Which of the following is true of the biologically important molecules ADP, AMP, and ATP?...
49. Which of the following is true of the biologically important molecules ADP, AMP, and ATP? A. all are both phosphoric anhydrides and phosphoric esters B. ADP and AMP are both phosphoric anhydrides and phosphoric esters, but ATP is only a phosphoric ester. C. AMP and ATP are both phosphoric anhydrides and phosphoric esters, but ADP is only a phosphoric ester. D. ADP and ATP are both phosphoric anhydrides and phosphoric esters, but AMP is only a phosphoric ester. 45....
12. If you used the PBLU plasmid as a vector to carry a desirable human gene into E. coli recipient cells. The insertion site is inside the B-galactosidase gene on the p-BLU plasmid. After the transformation, you plate the cells on LBA+amp+X-Gal to screen for E. coli cells with the desirable gene. You saw both blue and white colonies on the plate. Which type of colonies carry the desirable human gene? A. White B. Blue C. both D. neither one...
3. Name a procedure which can replace the heat shock process and achieve the same goal: 4. What is the purpose of adding ampicillin to LBA? A. LBA prevents contamination of the plates. B. Ampicillin is a growth factor that stimulates growth of E. coli cells. C. Ampicillin inhibits growth of E. coli cells that do not have amp gene. D. Ampicillin inhibits growth of E. coli cells harboring PBLU plasmid. 5. When B-galactosidase is present, X-Gal turns A. white...
1. On which plate(s) would you expect to find bacteria most like the E. coli on the source plate? Explain. 2. On which plate(s) would you find only genetically transformed bacterial cells? Why? 3. What is the purpose of the control plates? Explain the difference between the controls and why each one is necessary. 4. Why would one compare the -DNA/+Amp and DNA/+Amp plates?
number 2
Plasmid-small extrachromosomal dsDNA which contains genes that regulate non-essential life functions Transformation-acquired genes from free-floating DNA in the environment Competent cell-bacterial cell wall and cytoplasmic membrane become porous and allows transforming DNA to enter the cell 2. Explain why-control E. coli cells cannot grow on L.BA+amp and L.BA+amp+X-Gal. 3. goal: Name a procedure which can replace the heat shock process and achieve the same electroportation What in the ninnee of andinn amnicillin to I RA2
9. All activated 1ymphocytes become.... a. plasma cells b. effector cells. c. memory cells. d. cytotoxic cells e. effector and memory cellsob 10. The bacterial lung intection acquired by inhalation ot aerosols of contasinates water is known as M. a. whooping cough. b. walking preumonia. c. influenza. d. Legionnaires' disease 11. Mycobacteriun leprae is only grown in living cella. 5. Th a. True b. False 12. which of the following agento travel up the nerves to penetrate the cs a....
Question 10 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of arabinose on the expression of GFP, predict what will happen when we plate bacteria that are TRANSFORMED on plates containing ampicillin, but NO arabinose. the bacteria will grow and glow the bacteria will grow, but won't glow the bacteria will not grow Question 11 0.5 pts Thinking carefully about the genes on the plasmid, and the effect of arabinose on the expression of GFP,...
c. Lederberg & Tatum 23. A culture of a coli susceptible to ampicillin is transformed with a plasmid that conta ampiellin-resistance gene plus a gene of interest, c.g. the gene for the female hormone, estrogen coll is placed on a growth medium containing ampicillin, then a. no bacteria will grow. b, only ampicillin-sensitive bacteria will grow. c. only bacteria containing the plasmid will grow. d. all bacteria will grow. c. none of these the flavinanses except
2. Which of the following cells are leukocytes? a. NK cells b. neutrophils c. monocytes d. all of the above e. none of the above 3 Which of the following cells are lymphocytes? a. NK cells b. neutrophils c. monocytes d. all of the above e. none of the above 4. Why do complement proteins not damage healthy cells normally? a. Complement proteins must be produced by activated leukocytes. b. Complement proteins must be activated by enzymatic cleavage. c. Leukocytes...
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