DNA polymerase incorporates dNTP and not NTPs because : A. NTPs are low abundance in the...
DNA polymerase incorporates dNTP and not NTPs because :
A. NTPs are low abundance in the nucleus
B. NTPs cannot base pair with dNTPs
C. the 2’OH sterically inhibit NTPs from the active site
D. A and C
E. B and C
Homework Answers
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DNA polymerase incorporates dNTP and not NTPs because :
A. NTPs are low abundance in the...
NA primase is TRUE? 27. Which ONE of the following statements about DNA primase is in...
NA primase is TRUE? 27. Which ONE of the following statements about DNA primase is in polymerase cannot extend a new A) It is necessary for DNA synthesis because DNA polymerase canno strand in the 3 to 5' direction. B) It proofreads bases added by DNA polymerase. C) It is a type of RNA polymerase. D) It has terminal transferase activity. 28. How many of the following statements about PCNA are TRUE? • It encircles ssDNA. • It is an...
Mg2+ serves the following function(s) in the reaction catalyzed by DNA polymerase. Multiple answers allowed A....
Mg2+ serves the following function(s) in the reaction catalyzed by DNA polymerase. Multiple answers allowed A. It stabilizes the pentacoordinate transition state of the phosphodiester bond formation. B. It precipitates inorganic phosphate (Pi) arising from the reaction. C. It interacts with the 3'-hydroxyl of the primer and a phosphate in the dNTP. D. It stabilizes the negative charge on the departing pyrophosphate which is derived from the incoming dNTP. E. It interacts with the 3' hydroxyl of the incoming dNTP.
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A....
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A. Only ribonucleoside triphosphates contain the sugar ribose. B. Ribonucleoside triphosphates have low potential energy, making the polymerization reaction endergonic. C. Ribonucleoside triphosphates have high potential energy, making the polymerization reaction exergonic. D. Ribonucleoside monophosphates cannot form complementary base pairs with the DNA template. E. Ribonucleoside triphosphates are not used, rather all use deoxyriboside triphosphates. 14. How is a mutation in a bacterial cell that...
QUESTION 13 A new DNA strand elongates only in the to direction because A) DNA polymerase...
QUESTION 13 A new DNA strand elongates only in the to direction because A) DNA polymerase begins adding nucleotides at the 5' end of the template B) the polarity of the DNA molecule prevents addition of nucleotides at the 3'end OB) the C) replication must progress toward the replication fork D) DNA polymerase can add nucleotides only to the free 3'end QUESTION 14
helppp! low the possible pairing In the DNA double hedix secary stru b ase S o keep the distance between DNA de Belo...
helppp!
low the possible pairing In the DNA double hedix secary stru b ase S o keep the distance between DNA de Belo wa bax und all possible hydro d de site (e) Circle all lone pairs that could be hydro band e ception Note for Part The nitrogen atoms with three single bonds can electrons that could form a bonbonding per are involved in (b) Draw a dashed line between the likely hydrogen-bonding (e) Assuming that stronger hydrogen bonding...
3. Now, you repeat the procedure of question 2, but you add a complete dNTP mix...
3. Now, you repeat the procedure of question 2, but you add a complete dNTP mix (containing ATP, CTP, dGTP, and dTTP), and ddGTP. In this way, when the DNA polymerase incorporates a nucleotide carrying guanine into the growing DNA strand, it will use sometimes dGTP and sometimes ddGTP. When ddGTP is used, the DNApol will no longer add nucleotides to the DNA strand. For ala3, write below three the possible molecules produced. For b1-b3, consider that you used ddATP...
If you wish to sequence a long strand of DNA in one round of reactions, you...
If you wish to sequence a long strand of DNA in one round of reactions, you should: O A. Decrease the ddNTP/dNTP ratio O B. Increase the ddNTP/dNTP ratio O C. Use a shorter DNA primer O D. Add twice as much primer Based on this figure, the most likely error is: O A. The scientist forgot to add dNTPs to one of the reaction tubes. O O B. <label for="q7_4" id="lq7_4">The scientist did not denature the DNA strands</label> O...
Which statement about polymerase is false? Select one: a. The polymerase reaction is unique because it...
Which statement about polymerase is false? Select one: a. The polymerase reaction is unique because it was the first example of a biosynthetic reaction using a template b. DNA synthesis is a highly favorable reaction due to a 19 kJ/mol generated in the subsequent hydrolysis reaction of pyrophosphate by pyrophosphatase. c. Processivity is defined as the average number of nucleotides added before the polymerase dissociates. d. DNA polymerase requires a primer with a free 3-hydroxyl group. e. The DNA polymerase...
1. A DNA base pair is _____ forming_____ hydrogen bonds a. C with T : two...
1. A DNA base pair is _____ forming_____ hydrogen bonds a. C with T : two b. G with T : three c. A with C : three d. G with C : two e. A with T : two 2. the correct order of action of these enzymes in DNA replication is a. Helicase, primase, DNA polymerase, Ligase. b. transferase, primase, DNA polymerase , helicase c. DNA polymerase , ligase , helicase, primase d. ligase, primase , helicase ,...
Consider a standard PCR reaction. If instead of including double-stranded DNA from a biological sample, you ...
Consider a standard PCR reaction. If instead of including double-stranded DNA from a biological sample, you added mRNA molecules from a biological sample, the reaction would… Can you please explain why the answer is not E A. work, but would yield less product because you started off with single-stranded template B. A and D are correct C. work, but would generate much shorter products D. not work because you would also need to replace the dNTPs with NTPs for the...
NA primase is TRUE? 27. Which ONE of the following statements about DNA primase is in polymerase cannot extend a new A) It is necessary for DNA synthesis because DNA polymerase canno strand in the 3 to 5' direction. B) It proofreads bases added by DNA polymerase. C) It is a type of RNA polymerase. D) It has terminal transferase activity. 28. How many of the following statements about PCNA are TRUE? • It encircles ssDNA. • It is an...
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A. Only ribonucleoside triphosphates contain the sugar ribose. B. Ribonucleoside triphosphates have low potential energy, making the polymerization reaction endergonic. C. Ribonucleoside triphosphates have high potential energy, making the polymerization reaction exergonic. D. Ribonucleoside monophosphates cannot form complementary base pairs with the DNA template. E. Ribonucleoside triphosphates are not used, rather all use deoxyriboside triphosphates. 14. How is a mutation in a bacterial cell that...
QUESTION 13 A new DNA strand elongates only in the to direction because A) DNA polymerase begins adding nucleotides at the 5' end of the template B) the polarity of the DNA molecule prevents addition of nucleotides at the 3'end OB) the C) replication must progress toward the replication fork D) DNA polymerase can add nucleotides only to the free 3'end QUESTION 14
helppp!
low the possible pairing In the DNA double hedix secary stru b ase S o keep the distance between DNA de Belo wa bax und all possible hydro d de site (e) Circle all lone pairs that could be hydro band e ception Note for Part The nitrogen atoms with three single bonds can electrons that could form a bonbonding per are involved in (b) Draw a dashed line between the likely hydrogen-bonding (e) Assuming that stronger hydrogen bonding...
3. Now, you repeat the procedure of question 2, but you add a complete dNTP mix (containing ATP, CTP, dGTP, and dTTP), and ddGTP. In this way, when the DNA polymerase incorporates a nucleotide carrying guanine into the growing DNA strand, it will use sometimes dGTP and sometimes ddGTP. When ddGTP is used, the DNApol will no longer add nucleotides to the DNA strand. For ala3, write below three the possible molecules produced. For b1-b3, consider that you used ddATP...
If you wish to sequence a long strand of DNA in one round of reactions, you should: O A. Decrease the ddNTP/dNTP ratio O B. Increase the ddNTP/dNTP ratio O C. Use a shorter DNA primer O D. Add twice as much primer Based on this figure, the most likely error is: O A. The scientist forgot to add dNTPs to one of the reaction tubes. O O B. <label for="q7_4" id="lq7_4">The scientist did not denature the DNA strands</label> O...
Which statement about polymerase is false? Select one: a. The polymerase reaction is unique because it was the first example of a biosynthetic reaction using a template b. DNA synthesis is a highly favorable reaction due to a 19 kJ/mol generated in the subsequent hydrolysis reaction of pyrophosphate by pyrophosphatase. c. Processivity is defined as the average number of nucleotides added before the polymerase dissociates. d. DNA polymerase requires a primer with a free 3-hydroxyl group. e. The DNA polymerase...
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