Which one of the following best describes what fits into chymotrypsin's oxyanion hole? The Asp from...
The
following questions are based on the papin mechanism below:
Explain the role of each amino acid in the catalytic triad.
Site directed mutagenesis replacing the active site asp in the
papain protease with an ala showed that catalytic activity was not
significantly affected. Why would you think that asp is not so
critical in the cysteine proteases?
Hi R CH2 NH-R oe 0 hole oxyanion hole 0 oxyanion hole CH a)
Hi R CH2 NH-R oe 0 hole oxyanion...
5. Consider the following peptide: His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gin- Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr a. What are the fragments, if it is cleaved by trypsin? b. What are the fragments, if it is cleaved by chymotrypsin? c. What are the fragments, if it is cleaved by pepsin?
please explain each question thoroughly. thanks
Question 3: Arg-Cys-Met-Ala-Cys-Gly-Arg-Pro-Asn-Tyr-Leu-Trp-Ala-Ile-His-Phe-Ser-Cys-Lys a. What would happen if this peptide were to be incubated with dinitrofluorobenzene (FDNB) followed by 6M HCl hydrolysis at 1100C for 24 hrs. What labeled product(s) would be detected? Consider the following pepide: What would happen if the peptide were treated with CNBr? What would the products be? Why? b. What would happen if the peptide were treated with chymotrypsin? What would the c. products be? Why? Arg-Cys-Met-Ala-Cys-Gly-Arg-Pro-Asn-Tyr, Leu-Trp, Ala-Ile-His-Phe,...
Matching... Write the letter beside that which best describes the following. 1. _____ b-mercaptoethanol a. recognizes LVPRGS and cleaves the R-G peptide bond 2. _____ chymotrypsin b. purification technique used to separate proteins on the basis of charge 3. _____ trypsin c. utilizes DNA manipulation to co-express functional protein attachment 4. _____ cyanogen bromide d. purification technique used to separate proteins on the basis of size 5. _____ sodium dodecylsulfate e. is an example of an amphipathic molecule used...
all of them please
Question 12 (1 point) Which of the following conditions would kill amp' lac his bacteria? amp = ampicillin (an antibiotic), lac = lactose (a carbohydrate), his = histidine (an amino acid) A) growing the bacteria in media that contained ampicillin, had lactose as the sole metabolic carbon source, and contained the amino acid histidine. B) growing the bacteria in media that contained ampicillin, had lactose as the sole metabolic carbon source, but did not contain the...
IS. Which of the following best describes what happens when the price of oranges increases? a) There is a shift to the right in the demand curve for oranges b) There is a shift to the left of the demand curve for oranges c) There is a shift along the demand curve for oranges d) There is a no change in the demand curve for oranges 16. Which of the following best describes what happens when consumer income increases? a)...
Circle the correct answer. Which of the following statements best describes what would be expected to happen as you randomly add stocks to your portfolio? a. Adding more stocks to your portfolio reduces the portfolio's company- specific risk. b. Adding more stocks to your portfolio reduces the beta of your portfolio. c. Adding more stocks to your portfolio increases the portfolio's expected return. d. Statements a and care correct. e. All of the statements above are correct. Frank Lewis has...
Cyw Ala Ατα Ile Ausn Lou Asp aly Lys Ser Which molecule below is asubunit of peptide chains a. Acetic acid b. Alanine O. ATP d. Gracil Report the charge on most aqueous aspartie acid molecules at pH-7. Report the approximate p values for the amino acid whose titration curve is shown below (note there may be more slots for answer than answers). A) Value 1 B) Value 2 c) Value 3 c) Value 4 The structure of a rare...