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Review Questions 1. What is the function of the following components used in the separation of...
can you please answer all three questions. Thank you so much!! QUESTION 3: What is the...
can you please answer all three questions. Thank you so
much!!
QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks] QUESTION 1: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through...
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making...
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making 30 mL of 1.2% agarose in 1x TAE is: g agarose mL lX TAE 2. Briefly explain the roles of each of the following in electrophoresis. Agarose - Tris - EDTA- Power supply - 3. What is the stain we used to view DNA and how does it work? 4. Which samples amplified and which ones did not?
1.Describe the respective function of the agarose gel, electrophoresis buffer and power supply, in the set-up...
1.Describe the respective function of the agarose gel, electrophoresis buffer and power supply, in the set-up of electrophoresis. (1) 2.State three precautions in the process of setting up the digestion of DNA. (0.75) 3.To visualize the DNA bands on gel, one can use Midori-green or a DNA probe. a. Explain the underlying principle of both methods, respectively. (2.5) b. Suggest one advantage and disadvantage for each method, respectively. (1)
A student tried to run an SDS PAGE gel for protein separation in a lysate. He accidently used the...
A student tried to run an SDS PAGE gel for protein separation in a lysate. He accidently used the Tris, pH8.8, buffer for the stacking gel, and the Tris, pH6.8, buffer for the resolving gel. A. What would the protein bands look like when they reach the end of stacking gel, why? B. What would the protein bands like when they finish the SDS PAGE, why? C. If the student mistakenly used Tris, pH6.8, buffer for both stacking and resolving...
Chapter 10 Review: Biotechnology Learning Objectives and Application Questions Learning objectives are identified for you to...
Chapter 10 Review: Biotechnology Learning Objectives and Application Questions Learning objectives are identified for you to review your understanding of this topic. You are advised to reflect carefully on these objectives and check your own understanding to see if you have understood these essential concepts from this week’s reading. ANSWER THOROUGHLY all the application questions associated with each objective IN YOUR OWN WORDS. Learning Objective I: Evaluate the importance of biotechnology in modern societies (p. 228, 229) Application question #1...
CONCEPTUAL REVIEW MATERIALS Questions 1. What is a protein fingerprint? 2. Which is larger, a strand...
CONCEPTUAL REVIEW MATERIALS Questions 1. What is a protein fingerprint? 2. Which is larger, a strand of DNA or a single protein? 3. Does each type of cell make the same type of proteins? Why or why not? 4. Why do the proteins flow downward through the gel during electrophoresis? 5. Why do bubbles form on the electrodes in the electrophoresis set-up? What are the bubbles?
Petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml...
9,10,12
petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml of O.1 N HC from concentrated (12.0 M) HO tyou will be asked to make this solution as practicel & 100 ml of L.0 M Tris pt-76 MW of Tris base is 121 g/mole 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to or NaOH to adjust the pH to 7.67 to need HC...
please! Assay questions 5 points each 1-What RFLP stand for and what RFLP analysis test for?...
please!
Assay questions 5 points each 1-What RFLP stand for and what RFLP analysis test for? 2-Why do you add sample loading buffer to DNA sample prior to loading sample into agarose gel. 3-In cheek DNA extraction procedure, name two steps that helped to break (lysis) the cheek cells. 4- Would a shorter DNA fragment move faster or slower through agarose gel, why.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
Question 1. 1 2 3 4 5 6 7 8 Lane Name/Code Ladder 1B | 2B...
Question 1. 1 2 3 4 5 6 7 8 Lane Name/Code Ladder 1B | 2B 2 DNA Sample/Treatment DNA ladder Digest Digest Digest Digest Digest Digest 3B 4B 5B Negative Figure 1: 1% Super buffer agarose gel electrophoresis of restriction digest of the plasmid containing the gdhA gene insert. Based on the information above answer the following questions? 1. What ladder size used? 2. What are the two top bands and bottom bands representing? 3. Explain why the observed...
can you please answer all three questions. Thank you so
much!!
QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks] QUESTION 1: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through...
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making 30 mL of 1.2% agarose in 1x TAE is: g agarose mL lX TAE 2. Briefly explain the roles of each of the following in electrophoresis. Agarose - Tris - EDTA- Power supply - 3. What is the stain we used to view DNA and how does it work? 4. Which samples amplified and which ones did not?
A student tried to run an SDS PAGE gel for protein separation in a lysate. He accidently used the Tris, pH8.8, buffer for the stacking gel, and the Tris, pH6.8, buffer for the resolving gel. A. What would the protein bands look like when they reach the end of stacking gel, why? B. What would the protein bands like when they finish the SDS PAGE, why? C. If the student mistakenly used Tris, pH6.8, buffer for both stacking and resolving...
CONCEPTUAL REVIEW MATERIALS Questions 1. What is a protein fingerprint? 2. Which is larger, a strand of DNA or a single protein? 3. Does each type of cell make the same type of proteins? Why or why not? 4. Why do the proteins flow downward through the gel during electrophoresis? 5. Why do bubbles form on the electrodes in the electrophoresis set-up? What are the bubbles?
9,10,12
petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml of O.1 N HC from concentrated (12.0 M) HO tyou will be asked to make this solution as practicel & 100 ml of L.0 M Tris pt-76 MW of Tris base is 121 g/mole 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to or NaOH to adjust the pH to 7.67 to need HC...
please!
Assay questions 5 points each 1-What RFLP stand for and what RFLP analysis test for? 2-Why do you add sample loading buffer to DNA sample prior to loading sample into agarose gel. 3-In cheek DNA extraction procedure, name two steps that helped to break (lysis) the cheek cells. 4- Would a shorter DNA fragment move faster or slower through agarose gel, why.
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
Question 1. 1 2 3 4 5 6 7 8 Lane Name/Code Ladder 1B | 2B 2 DNA Sample/Treatment DNA ladder Digest Digest Digest Digest Digest Digest 3B 4B 5B Negative Figure 1: 1% Super buffer agarose gel electrophoresis of restriction digest of the plasmid containing the gdhA gene insert. Based on the information above answer the following questions? 1. What ladder size used? 2. What are the two top bands and bottom bands representing? 3. Explain why the observed...
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